Sentences with phrase «into zebrafish»

Researchers at Duke University Medical Center transplanted a set of human genes into a zebrafish and then used it to...

The results of our efforts were published recently in a paper entitled Incorporating RNA - seq data into the Zebrafish Ensembl Gene Build in Genome Research.

To differentiate the two possibilities, the researchers dropped a dose of the anti-angiogenesis drugs semaxinib and pazopanib — already used to treat cancer — into the zebrafish's water.

A synthetic form of the snail insulin, when injected into zebrafish, caused blood glucose levels to plummet.

Most notably, the studies are based on transient transfection of wild - type and mutant gene constructs into cultured cells or into zebrafish embryos and are unlikely to reproduce the specific mutant gene dose that is present in heterozygous mutant cells in FOP patients.

Wherever they injected RNA for the trio into the zebrafish embryos, tails popped up, the team reports in the 24 July issue of Nature.

It is caused by a genetic mutation, which Dr. Baraban's group was able to introduce into the zebrafish to cause epilepsy.

An RNA cocktail injected into a zebrafish embryo produces an abnormally placed second tail.

The group also plans to follow up on other proteins secreted after injury that were identified in their initial search, which may provide additional hints into the zebrafish's secrets of regeneration.

Going a step further, the researchers transplanted the cavefish mutation into zebrafish and confirmed that it contributes to both insulin resistance and weight gain.

When the scientists inserted human colorectal cancer cells into zebrafish embryos and allowed them to grow for 4 days, the resulting tumors showed three hallmarks of human solid tumors: rapid cell division, formation of blood vessels to supply nutrients, and the ability to spread to other locations in the body.

Back in the aquatic suites, veterinarian Jennifer Lofgren is peering into a zebrafish tank.

Not exact matches

McGlinn will work within the supportive environment of ARMI, where 54 researchers are prying into the mysteries of vertebrate regeneration by studying sharks, mice, chickens, axolotls, and zebrafish.

For the study, the researchers created a line of zebrafish with defective immune systems by inserting a mutation into a gene required for development of an important component of the immune system.

So Farber and Quinlivan and their team were actually able to see the fats that they fed their zebrafish glowing under the microscope as they were broken down and reassembled into new molecules in different organs.

Studies found that a derivative excreted by juiced cattle reduces minnows» fertility, transforms female zebrafish into males, and induces other masculinizing effects.

Scientists have now discovered that these viruses have integrated themselves into the DNA of a wide range of animals, including humans, zebrafish, and other vertebrates.

Zebrafish in which ctgfa was disabled had glial cells that often failed to extend into the lesions, and the fish were unable to recover from spinal injury.

In the study, the researchers used zebrafish lines that had been selected to be bolder by breeding fish that stayed still for a maximum of 50 seconds after being introduced into new surroundings, while shy fish — those that stayed still for more than 3 minutes when dropped into a new area — were bred to create a shy line.

Building on the evidence that microglia were in play during injury, the researchers conducted tests in zebrafish with the specialized enzyme incorporated into both rod cells and microglial cells, removing both cell types to ask what role microglia play during regeneration.

Finally, they demonstrated that zebrafish OPCs differentiate into mature oligodendrocytes when cultured together with human motor neurons, differentiated from induced pluripotent stem cells.

In another series of experiments, they inserted a green fluorescent protein into the tissues that give rise to blood and lymph vessels in embryonic zebrafish.

«Our hope is that by creating this system, we can continue our own investigations into other harmful organisms, and that other scientists worldwide can adapt our zebrafish model to investigate the diseases that are priorities in their communities,» Perfect said.

So they injected the organism into microscopic zebrafish larvae, which have clear bodies, and watched the infection take hold.

Researchers discovered a gene also found in humans helps zebrafish convert water motion into electrical impulses that are sent to the brain for perception.

«Regenerative potential of cells in mouse retina: Researchers use a clue from zebrafish to discover the cues that reprogram Müller glia into retinal neurons.»

For the first time, it is now found by a study that various mechanisms of transcribing DNA into RNA exist during gene expression in the different development phases of zebrafish.

Scientists from the Max Planck Institute for Heart and Lung Research in Bad Nauheim, together with U.S. colleagues, have now observed in the embryo of the zebrafish that muscle cells migrate from the undamaged atrium into the ventricle and thus significantly contribute to regeneration.

The zebrafish is used as a model organism for research into the embryonic development of vertebrates.

To investigate how zebrafish tails take shape, Thisse's team removed some of the presumed pre-tail cells from early - stage embryos, then transplanted them into the middle of another embryo.

This approach opens new avenues for research into memory using zebrafish as model organism,» concludes Dr. Okamoto.

Microinjection of wild - type human ACVR1 RNA into alk8 — / — zebrafish embryos rescued approximately 80 % of the injected embryos completely or partially (Figure 3, C and F), showing that human ACVR1 can function as a BMP type I receptor in this zebrafish model and substitute for Alk8.

Durdu, a PhD student in Darren Gilmour's lab at EMBL, found this behaviour in specific groups of cells in the zebrafish: the cells that will develop into the animal's «lateral line», a series of ear - like organs along the fish's flank that allow it to sense changes in water pressure.

This information would then be added into the core Ensembl gene - set (the reference zebrafish genome used by researchers around the world) in such a way as to increase the quantity and quality of the gene - set without adding artifacts or pseudogenes.

In the case of zebrafish, less than 20 percent of fish produced by injecting DNA into fertilized eggs will transmit the foreign gene to the next generation.

By the third day, all of its major organs have fully developed and moved into proper position, offering scientists a view of what has been referred to as the zebrafish's «artful etchings.»

These feeder cells, derived from zebrafish testicular cancer cell lines, promote the growth of spermatagonia and stimulate them to mature into functional sperm,» said Dr. Sakai, the study's senior author and a reproductive biologist.

To identify which cells actually filled in excised zebrafish heart muscle, Izpisúa Belmonte's team first employed some genetic engineering to only make cardiomyocytes «transgenic» by inserting into them a tracer gene that made them glow green under a microscope.

«Discovery of a gene that could convert human embryonic stem cells into myocardial cells would be golden,» said Didier Stainier, PhD, UCSF assistant professor of biochemistry and biophysics, the senior author of the UCSF study and a pioneer in the study of heart development in the transparent zebrafish embryo.

To better test the effects on locomotor activity in response to certain compounds, zebrafish larvae can be loaded into multi-well plates.

Zebrafish larvae (96 hpf) were loaded into a 96 - well plate and run through a light - dark test in the observation chamber.

Leprosy hijacks our immune system, turning an important repair mechanism into one that causes potentially irreparable damage to our nerve cells, according to new research that uses zebrafish to study the disease.

«IKKs are one of the most heavily sought after drug targets today, because pharmaceutical companies want safe and effective medications to treat the pain of arthritis, now that the safety of COX - 2 inhibitors, like Celebrex and Vioxx, have been called into question,» says Salk researcher Vinay Tergaonkar who was involved in the zebrafish study.

Zebrafish Provides Insights into New AMD - Associated Genetic Mutation Philippe Mourrain, PhD Stanford University (Stanford, CA)

Initial work at the firm will involve research into a particular drug that, according to tests, can double the rate at which complex tissues in zebrafish regrow after amputation.

In this study, the team delved deep into the nucleus of cells belonging to mouse and zebrafish embryos — two important animal models of embryonic development — in order to determine how the Dll4 gene is turned on.

Zebrafish mesoderm progenitors are brought into contact before being separated by pulling the micropipettes apart while applying increasing pressures in a stepwise fashion with the left micropipette.

In a report published on September 30 in Nature Genetics, Katsanis and his team used a small transparent fish, zebrafish, to literally watch what happened if they chemically blocked the production of three proteins that are required for primary cilia function during the period when a fish egg develops into a grown up, fully - finned fish.

Looking through a microscope, Youngnam Jin — a postdoctoral fellow in Randy Peterson's lab at Massachusetts General Hospital's Cardiovascular Research Center — arranges one - celled zebrafish embryos, each about 10 minutes old, into rows of 20, spinning them with a micro spatula to turn their nuclei toward the ultrathin glass needle of a micro-injection device.

To demonstrate Amrose functionality in an established higher model system, Amrose v0 — v4 variants were cloned into the pCS2 + vector system to synthesize mRNA by in vitro transcription, which was used for injection into one - cell stage zebrafish embryos.