In the present study, we used protein engineering of Kivd to improve
isobutanol production in Synechocystis PCC 6803.
Single replacement, either Val461 to isoleucine or Ser286 to threonine, increased the Kivd activity significantly, both in vivo and in vitro resulting in increased overall production while
isobutanol production was increased more than 3 - methyl -1-butanol production.
Using their light - switch technique, the researchers set out to keep the yeast alive while maximizing
isobutanol production.
The researchers predicted they could use a combination of genetic engineering and light to fine tune
isobutanol production.
Not exact matches
Efforts to make
isobutanol from bacteria alone have been underway since 2000 when the U.S. Department of Agriculture and the U.S. Department of Energy (DOE) began distributing grants to universities that could demonstrate successful
production of liquid biofuels.
However, most attempts to create
isobutanol biofuel have run into difficulties involving cost or scaling
production to an industrial level.
Using their new technique, the Princeton researchers have now used fermentation and genetically - engineered yeast to produce other chemicals including lactic acid, used in food
production and bioplastics, and
isobutanol, a commodity chemical and an advanced biofuel.
But while those enzymes are active, ones that influence the
production of
isobutanol can't work.
Moreover, among all the engineered strains examined, the strain with the combined modification V461I / S286T showed the highest (2.4 times) improvement of
isobutanol - to - 3M1B molar ratio, which was due to a decrease of the activity towards 3M1B
production.