Sentences with phrase «isoforms with»

Each of these proteins possess several isoforms with characteristics that either mimic or antagonize the role of p53.

ABP is a protein closely related to GRIP and exists in two isoforms with 6 and 7 PDZ domains, respectively.

OPTN has 3 isoforms with MW 66 、 65 and 60 kDa (refer to UniProt).

HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

We report here that in vitro generated DS neural cells have abnormal metabolism of amyloid - β (Aβ) manifested by increased secretion and accumulation of Aβ granules of Aβ42 pathological isoform with upregulated expression of the APP gene.

Not exact matches

In the current study, deploying TFPI and an isoform of tenascin C, TNC - FN IIIC, with CA 19 - 9 improved performance discriminating stage I and II disease from healthy or benign disease controls.

«We found that the protein isoforms expressed in African Americans with prostate cancer do not always respond to targeted therapies, whereas these drugs were found to be effective in European Americans with prostate cancer and do end up killing off the cancer,» said Lee.

In mice lacking the NDST1 gene, and therefore the NDST1 enzyme, nature substitutes with an NDST1 isoform (NDST2, NDST3, and NDST4), but the results — like substitutions in cooking — are noticeable.

The roadmap outlines future research directions toward the goal of enhancing human radioresistance, including upregulation of endogenous repair and radioprotective mechanisms, possible leeways into gene therapy in order to enhance radioresistance via the translation of exogenous and engineered DNA repair and radioprotective mechanisms, the substitution of organic molecules with fortified isoforms, the coordination of regenerative and ablative technologies, and methods of slowing metabolic activity while preserving cognitive function.

«Last year, we reported analogous findings with microRNA isoforms.

When the PP2A catalytic C subunit associates with the A and B subunits several protein isoforms, namely holoenzymes, are produced with distinct activity and substrate specificity.

At SAGE KE (http://www.sciencemag.org/sciext/drugdisc/#insageke), Raber describes the role of isoform - dependent interactions of apoE with androgens and androgen receptors in the progression of Alzheimer's disease.

In the course of screening over 180 mutant mouse strains with a systematic battery of behavioral tests, Dr. Tsuyoshi Miyakawa and his colleagues found that mice with heterozygous knockout of the alpha - isoform of calcium / calmodulin - dependent protein kinase II (αCaMKII) exhibit behavioral deficits and other brain features consistent with bipolar disorder.

SAM can be combined with a human genome - wide library to activate all 23,430 known coding isoforms from the RefSeq database.

In vitro, transforming human brain neuroglioma or Chinese hamster ovary cells with an amyloid - β isoform resulted in better survival following a yeast infection.

Whereas acute CD8 + T cell effector responses are enhanced in DGKζ - deficient mice postinfection with intracellular pathogens, persistent memory formation is impaired, most dramatically when both DGKζ and DGKα, the other isoform of DGK that metabolizes DAG downstream of the TCR in T cells, are deleted (35, 36).

I have begun to investigate BIN1 expression and the isoform diversity in the brains of patients with AD.

The GFP - tagged isoform a (plasmid pCK28 -LCB- PW01A8.1:: W01A8.1 (a) synth:: gfp:: unc - 54 3 ′ UTR -RCB--RRB- was constructed by synthesizing the W01A8.1 a sequence with modified codons to allow protection from CRISPR / Cas9 targeted sgRNA and prepared as a GeneArt ® Strings ™ DNA Fragment from Invitrogen (Invitrogen, Carlsbad, California, USA) and cloned using GeneArt ® Seamless Cloning System (Invitrogen) into pPD95.75 (NeoR).

(C) Gel image of RT - PCR results showing multiple transcript isoforms of Bsl1 in bsl1 - 2 inflorescence primordia at 15 DAS compared with A10.1.

Laminin - 521 induce fast migration of the cells and laminin isoform specific activation of PI3K / Akt pathway through interaction with α6β1 integrin.

LAMscreen is a kit with all our currently available laminin isoforms so that you can easily screen the best laminins for different cell types.

Using surface plasmon resonance experiments, an in vitro method for determining molecular binding partners, the researchers found that both ApoE4 and ApoE3 — an ApoE isoform associated with a lower risk of AD compared to ApoE4 — bound the SIRT1 promoter.

Calculate isoform ratios for each gene: For a gene G, with k isoforms I, the splicing ratio is defined as:

Using the yeast two - hybrid screen, we have isolated two clones that specifically interact with the three isoforms of Rab6 present in mammalian cells (Rab6A, A» and B).

Here we use a chemical genetics approach using knock - in mice in which either p38alpha or p38beta kinase has been rendered resistant to the effects of specific inhibitors along with p38beta knock - out mice to dissect the biological function of these specific kinase isoforms.

NMNAT2 is the major NMNAT isoform expressed in the mammalian brain and is extremely labile with a short half - life in neurons.

Because the polyT repeat is reported as the key variant to define TOMM40 clades A and B, 12 we used this marker and APOE isoform information to perform analyses based on phylogenetic groups as described by Roses et al. 12 Haplotype phase was estimated using PHASE software.22 The phylogeny, which represents the evolutionary relatedness of the haploytpes, was estimated using neighbor - joining with 10 000 bootstrapping replicates in the CLC DNA workbench (CLC bio, Aarhus, Denmark)(Supplemental Figure 3; http://neuroscienceresearch.wustl.edu/Pages/cruchaga2011.aspx).

We replaced exon 3, which encodes a part of the RNA binding domain and is present in all Boule isoforms (Figure 3C), with a lacZ - neo vector through homologous recombination in embryonic stem cells.

More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3 ′ untranslated regions (3 ′ UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms coordinating alternative 3 ′ - UTR processing for specific mRNA populations remain poorly defined.

Lane 1, wild type (N2); lanes 2 and 3, unc - 7 (e5) rescued with unc - 7S:: gfp + cosmid F56B12, expected to express all isoforms; lane 4, unc - 7 (e5) rescued with unc - 7SΔ1S + F56B12, predicted to express UNC - 7L:: and SR:: GFP.

In a genome - wide analysis of up to 17 503 participants in PROMIS, we identified genetic variants associated with either apolipoprotein (a) isoform size or lipoprotein (a) concentration.

It therefore appears unlikely that UNC - 9 heteromerizes with either UNC - 7S or L isoforms.

The clearest finding from proteomics experiments is that most human genes have a single main protein isoform, while those alternative isoforms that are identified tend to be the most biologically plausible: those with the most cross-species conservation and those that do not compromise functional domains.

In 60 801 patients with coronary heart disease and 123 504 controls, OR for myocardial infarction was 0 · 96 (0 · 94 - 0 · 98; p < 0 · 0001) per 1 - SD increment in apolipoprotein (a) protein isoform size in serum due to rs2457564, which was directionally concordant with the OR observed in PROMIS for a similar change.

This could be interpreted as UNC - 7L and UNC - 9 forming heteromeric channels, with ion selectivity properties that are influenced by both isoforms (as this dictates the rectification of Gj0), but Vj gating properties dominated by UNC - 7L (as this dictates the shape of the Gj ∞ plot).

Methods: In this mendelian randomisation study, we measured lipoprotein (a) concentration and determined apolipoprotein (a) isoform size with a genetic method (kringle IV type 2 [KIV2] repeats in the LPA gene) and a serum - based electrophoretic assay in patients and controls (frequency matched for age and sex) from the Pakistan Risk of Myocardial Infarction Study (PROMIS).

Lipoprotein (a)- lowering interventions could be preferentially effective in reducing the risk of coronary heart disease in individuals with smaller apolipoprotein (a) isoforms.

Finally, we compared phenotypic versus genotypic ORs to estimate whether apolipoprotein (a) isoform size, lipoprotein (a) concentration, or both were causally associated with coronary heart disease.

The p63 bands migrated to 60 kDa, which is consistent with the TAp63 isoform.

Rescue of forward (F) or reverse (R) locomotion by plasmid constructs, with or without co-injection of F56B12, was determined (isoforms predicted to be expressed are listed in red).

Cortisone is produced by the action of 11β - hydroxysteroid dehydrogenase (11β - HSD), an enzyme with two isoforms, the first of which, 11β - hydroxysteroid dehydrogenase I (11β - HSD I) catalyzes cortisone into cortisol, enabling rapid supply of the active hormone as needed.

The E3 isoform has been proven to bind to tau (with the same suspected for E2), thus preventing or minimizing its phosphorylation.

Thus, although there are only three isoforms expressed in human skeletal muscle, there are many more hybrid muscle fiber types comprising muscle fibers with several different isoforms within the same muscle fiber (see review by Scott et al. 2001).