Sentences with phrase «isogenic hipsc»

This study defined about 200 negative genetic interactions in the isogenic cancer cell line background.

This study defines a network of synthetic sick / lethal interactions with a set of query genes in a series of isogenic cancer cell lines.

Near - isogenic lines for measuring phenotypic effects of DIMBOA - Glc methyltransferase activity in maize

Demonstrated that laboratory - adapted CCR5 - tropic 81A virions fuse rapidly and efficiently to immature MDDCs, whereas NL4 - 3, the isogenic CXCR4 - tropic counterpart of 81A, fuse slowly and inefficiently to both immature and mature MDDCs.

There are a large number of laboratory strains available, and their long breeding history means that mice of a single laboratory strain are isogenic.

Epigenetic modifications in bacteria, such as DNA methylation, have been shown to affect gene regulation, thereby generating cells that are isogenic but with distinctly different phenotypes.

These isogenic cell lines can be used not only to understand the cellular consequences of disease mutations, but also to perform high throughput genetic and pharmacological screens to both understand the underlying pathological mechanisms and to develop novel therapeutic agents to prevent or treat such diseases.

The ability of the ospC7 / ospC +4 clone to infect mice was tested by needle - inoculation with a moderate i.p. / s.c. dose of 5 × 103 ospC7 / ospC +4 organisms, in parallel with the isogenic B31 - A3 WT and ospC7 mutant clones (Table 1).

For example, a transcription factor equally prevalent in two isogenic cells might be bound to a promoter in one and unbound in another, subject to the dictates of statistical mechanics.

In order to directly compare these two strains in Drosophila melanogaster, we used Wolbachia - infected lines in a genetically identical DrosDel w1118 isogenic background [39].

The 1st and 3rd chromosome of the D. melanogaster stock with wAu were replaced with DrosDel w1118 isogenic chromosomes using a first and third double balancer line.

Also, pESC lines, that carry isogenic genome information from the oocyte donors, can be derived and may represent a potential alternative source of stem cells for basic scientific studies as well as novel therapeutic application.

Since the three colonies are isogenic, differences between them could explain the observed morphological discrepancies.

His lab develops isogenic human pluripotent stem cells and transgenic animals to model disease, with the goal of delineating novel approaches to influence outcomes for Huntington disease (HD) and Fragile X Syndrome (FXS), the most common genetic causes of dementia and intellectual disability, respectively.

Publishing in Scientific Reports researchers report NK603 genetically modified maize and its isogenic control are not substantially equivalent.

To create cell models for the discovery of disease etiology, progression, and treatment, isogenic cell lines can be created from healthy or sick individuals, then compared (Figure 1).

Discovery that induced stress responses underlie inter-individual variation in isogenic animals and promiscuously reduce the effects of inherited detrimental mutations (Lehner group, Science 2012).

An intact genome, isogenic hiPSC model from an individual with mosaic Down syndrome reveals neurogenesis and mitochondrial defects caused by Trisomy 21

Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.

Genetic Analysis Using an Isogenic Mating Pair of Aspergillus fumigatus Identifies Azole Resistance Genes and Lack of MAT Locus's Role in Virulence.

Xl280 is a haploid F1 progeny descended from a cross between two exceptionally well - validated haploid sibling parental strains, whose genomes have been both sequenced, B3501α and JEC20a (which is isogenic to the sequenced strain JEC21α, with the exception of the mating - type locus)(Figure 1A)[34], [45]--[47].

Pairwise tests among six isogenic lines ofDrosophila melanogaster were performed to determine whether there is a genotype - specific interaction in the success of sperm.

All these cells were isogenic, which means they all had the same gene set.

Bacterial populations pose an intriguing puzzle: in so - called isogenic populations, all bacteria have the same genes, but they still behave differently, for example grow at different speeds.

Using this approach, researchers can easily generate diseaserelevant isogenic models to determine the impact of correcting or introducing disease - relevant mutations on cellular phenotypes in a tissue - appropriate context.

They verified that these epilines were indeed isogenic, or genetically identical, by sequencing the genome of each.