Sentences with phrase «isolate islet cells»
They also developed a unique cell - sorting technique to isolate islet cells from other cells in the pancreas.
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Investigators found that autophagy plays a role in clearing IAPP from pancreatic beta cells using three experimental models: pancreatic beta cells, isolated pancreatic islets from mice that express the human form of IAPP, and human islets.
Interestingly, isolated islets provide a superior platform for studying propagation of IAPP aggregates because islets retain the composition and organization of endocrine cells and the extracellular matrix, which is absent in isolated, cellular monolayers.
ASCs isolated from the stromal vascular fraction of fat have advantages over other mesenchymal stem cell sources; they are easy to isolate and expand and aid in the repair of damaged tissues [7], including islet graft survival and revascularization [8].
On the other hand, a Brs3 agonist promoted insulin secretion in both rodent insulinoma cell lines and in islets isolated from wild - type but not Brs3 mutants [53].
Unfortunately, conventional sorting techniques have not been sufficient to isolate the individual islet cell types, making it impossible to analyze each one's gene expression program and its potential contribution to dysfunction.
To overcome this limitation, a team led by JAX Assistant Professor Michael Stitzel, Ph.D., isolated single cells from the pancreatic islets of both non-diabetic and type 2 diabetic subjects to obtain transcriptomes for each cell.