Sentences with phrase «isolate populations of these cells»

She works to isolate populations of these cells, discover how the body regulates them, and understand how they can be used to treat disease.

Not exact matches

Using fluorescent markers, Fuchs and her colleagues isolated two distinct populations of stem cells from mouse skin.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

«Those approaches take a long time, it is difficult to isolate a pure population, and the NPC - like cells are still transient,» says Zhongwei Li, a research associate in the Izpisua Belmonte lab and co-first author of the new paper.

Among the three isolated cell populations, the relative gene expression of macrophage markers was highest among the F4 / 80 + cells.

Expression analysis of macrophage and nonmacrophage cell populations isolated from adipose tissue showed that adipose tissue macrophages are the primary sources of TNF - α and other proinflammatory molecules in adipose tissue.

The results of our limiting dilution analysis confirmed that the isolated CSCs (CD24 − / CD44 + / ESA +) population has significantly stronger ability of generating tumors compared with non-stem cell population (Table 1).

To determine whether adipose tissue macrophages express any molecules implicated in obesity - associated complications, we isolated three cell populations from the parametrial adipose tissue of three obese B6.V Lepob / ob mice: (a) an adipocyte - enriched population, (b) a stromal vascular macrophage F4 / 80 + population, and (c) an F4 / 80 — stromal vascular population.

After this period of induction, keratinocytes derived from pluripotent stem cells were isolated and amplified to obtain a homogenous and pure population of keratinocytes presenting all the phenotypic characteristic of adult keratinocytes.

If the User requests isolated cells, Users will provide an account number for a charge - back of $ 100 for each hepatocyte / Kupffer cell isolation, to cover the cost of biochemicals, gradients and cell culture media required for isolation of purified cell populations.

Flow cytometry was employed to isolate the sorted population of CD81 - negative cells.

Being able to isolate stable populations of totipotent cells in culture would therefore be highly valuable for research and have potential medical significance.

Comparison of the differentiation potential of cell populations isolated from human lipoaspirate, trabecular bone tissue and periosteal tissue.

Dr. Studer and colleagues isolated and cloned a population of neural rosette cells (R - NSCs), which have an expanded neuronal subtype differentiation potential.

A review in the journal Cell Stem Cell by two Jackson Laboratory (JAX) scientists reports on the latest efforts to isolate and culture the elusive populations of stem cells that most closely resemble very early (two - cell stage) totipotent ceCell Stem Cell by two Jackson Laboratory (JAX) scientists reports on the latest efforts to isolate and culture the elusive populations of stem cells that most closely resemble very early (two - cell stage) totipotent ceCell by two Jackson Laboratory (JAX) scientists reports on the latest efforts to isolate and culture the elusive populations of stem cells that most closely resemble very early (two - cell stage) totipotent cecell stage) totipotent cells.

These findings strongly suggest that we have succeeded in isolating a unique metastatic CSC population by exposing breast cancer cells to repetitive cycles of hypoxia and reoxygenation.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

However, as demonstrated by a recent report from Stuelten and colleagues [13], the complexity of CSC markers continues to pose challenges for identifying and isolating the putative tumor stem cell populations by the cell - sorting approach.

Starting with transplants of human oligodendrocytes in the late 1980s [40], and more recently with populations of human oligodendrocyte progenitor cells isolated from the developing or adult CNS, or from human embryonic stem cells, it has been possible to generate extensive myelination upon transplantation into spinal cord injury or into congenital mouse models of hypomyelination [41]--[48].

As a first step towards determining whether human glial progenitor cells (hGPCs) can generate functionally distinct astrocyte populations, we exposed embryonic hGPCs isolated from spinal cords of 9.5 week old abortuses to BMP or CNTF.

After that year, the researchers may continue to work on any stem cell lines (the isolated populations of stem cells) that they have established.

To our knowledge, this study is the first to identify a pluripotency marker in a heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming.

Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.

Subsequently, we isolated (through FACS) and cultured the top 10 % and bottom 10 % of the SSEA3 / 488 fluorescing cells as our SSEA3 - positive and negative populations respectively (Figure 2B).