«We studied human T cells,
isolated from blood donors of all ages, to compare mature cytotoxic T cells with naive ones,» said Philip Ansumana Hull, graduate student in Ott's lab and one of the first authors of the study.
Scientists have long thought that HIV infects only memory T cells, based on studies of T cells
isolated from blood.
The skin assay has been developed using cells
isolated from blood samples from a range of healthy volunteers.
DNA samples were
isolated from blood cells, amplified by PCR and digested with Tru1l.
DNA was
isolated from blood and bone marrow samples using the QIAamp DNA Blood Maxi Kit (Qiagen, Valencia, CA), and cDNA was synthesized from RNA using random hexamer priming.
Here, we performed whole - genome sequencing of paired
isolates from the blood and CSF of 869 meningitis patients to determine whether such variation frequently occurs between these two niches in cases of bacterial meningitis.
Plasma miRNA was
isolated from blood samples of 27 enrolled dogs divided into three groups: normal dogs with no age limit, dogs with asymptomatic MMVD, and dogs with MMVD - CHF.
Plasma Ex-miRNA was
isolated from blood samples from 27 enrolled dogs divided into four groups: young, normal dogs less than 7 years old; old, normal dogs 7 years old or older; dogs with asymptomatic MMVD; and dogs with MMVD - CHF.
Both blood and urine will be used for diagnostic testing, and DNA will also be
isolated from the blood for genetic analysis in order to detect possible mutations that are causing the disease.
The paramyxo virus can thus be
isolated from blood and culturing can reveal the exact condition a dog may be experiencing.
Not exact matches
Instead, the industries we
isolated are roughly ranked
from best bets (environmental consulting) to slightly more risky ventures (
blood banks and irrigation systems).
Researchers are actively at work creating less invasive tests to screen for chromosome disorders in developing babies, such as by
isolating the baby's genetic material
from samples of the mother's
blood.
While the Vortex system is designed specifically for
blood, the company is working with academic researchers to develop versions that could be used to
isolate cells
from other body fluids as well, such as cerebrospinal fluid and urine.
They
isolated blood cells
from HIV - positive patients on antiretroviral therapy and at different stages of disease progression, as well as cells
from non-infected individuals.
Transgenic antithrombin was intended to improve supply and address concerns about pathogens in the form of the drug
isolated from human
blood.
Using a new analytical method, they identified which vertebrate species the bugs had parasitized by
isolating fragments of DNA left over
from blood meals.
Researchers have
isolated exosomes
from tumors and
from blood of patients with breast cancer, and
from blood of mice with human tumors grown after breast implantation in mice, called ortoxenogratfs.
Here, Qihui Wang and colleagues
isolated 13 monoclonal antibodies
from the
blood of a Zika virus - infected patient returning
from Venezuela to China.
Not so long ago, the
blood - brain barrier was thought to
isolate the brain
from the immune system.
First, IgE antibodies
from the patient's
blood are
isolated by interaction with magnetic beads that are coated with a different type of antibody.
Both groups
isolated carefully characterized
blood stem cells
from genetically marked mice.
Umbilical cord tissue - derived cells (hUTC) differ
from umbilical cord
blood cells in that they are
isolated from cord tissue itself, rather than the
blood.
The researchers also analyzed specific immune cells called cytotoxic T cells
isolated from the patients»
blood and found increases in biomarkers indicative of immune activation.
Although Wyss - Coray's group has not yet
isolated any rejuvenation factors
from the young
blood, they have found what might be an alternative.
The body releases chemicals that cause
blood vessels to leak and tissues to swell in order to
isolate a foreign substance
from further contact with the body's tissues.
She adds that her team is finding more than collagen: It has recovered sequences
from eight proteins
isolated from what appear to be
blood vessels, all matching common vessel proteins such as actin, tubulin, and hemoglobin.
More recently, Wagers's lab, together with cardiologist Richard Lee's lab at Brigham and Women's Hospital in Boston,
isolated a specific protein
from mouse
blood that they think can explain some of the effects.
«He identified a type of molecular sensor, which programmed T cells
isolated from human
blood with customized instructions for thwarting attack.
Researchers have
isolated stem cells
from only some of the body's organs and tissues, including the
blood, brain, skeletal muscle, heart muscle and most recently
from skin.
The scientists drew
blood from old mice, whose supply of T cells was depleted, and
isolated immune cells.
In the current study, Vanderbilt researchers led by graduate student Andrew Flyak, the paper's first author, used a high - efficiency method they developed to quickly
isolate and generate large quantities of monoclonal human antibodies
from the
blood of survivors of a 2007 outbreak in Uganda who were infected by the Bundibugyo ebolavirus.
DNA was
isolated from each patient's
blood and analyzed for the presence of any of six candidate genetic variations in a panel of scar formation and wound - healing genes.
In a pilot test, for example, users
isolated malaria parasites
from blood for detection under a microscope with 15 minutes of spinning, a time frame comparable to commercial centrifuges.
Concerned that the drug by itself might not keep his aggressive cancer at bay, Wartman opted for a second transplant — this time with stem cells
isolated from peripheral
blood from an unrelated donor.
For the animal experiments, Savio Woo of the Center for Gene Therapy at Baylor College of Medicine in Houston and his colleagues first
isolated liver cells
from transgenic mice that produce the human protein a1 - antitrypsin in their livers,
from where it is secreted into the
blood.
In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells
from a human intestinal cell line originally
isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane
from human
blood vessel - derived endothelial cells in the adjacent channel.
Using a new method, the scientists therefore
isolated T cells specifically programmed to target the CMV virus
from the
blood of the donor and transferred small numbers of these cells to the patients.
In the June issue of the journal Vaccine, Christopher Blyth at the University of Western Australia and colleagues found that Fluvax (but not other flu vaccines) triggered the release of high levels of cytokines in
blood cells
isolated from the children who had febrile seizures.
To test that exosomes were the source of this brain to body communication, Haughey's research team
isolated exosomes
from the
blood of mice four hours after injecting the cytokine IL - 1b into brain and then injected the exosomes into the tail veins of different mice that had the cytokine and the nSMase - blocking drug altenusin already in their brains.
The researchers used an enhanced version of a technique they developed called CAPP - Seq to
isolate and sequence circulating tumor DNA, or ctDNA,
from blood samples
from the patients.
Early animal studies have shown that stem cells
isolated from umbilical cord
blood can stimulate cells in the spinal cord to regrow their myelin layers, and in doing so help restore connections with surrounding cells.
The reason, according to the judge, is that the patent poses a «risk of preempting [other uses of] the natural phenomenon» that the test focuses on — fetal DNA that can be
isolated from the mother's
blood.
Johannes Scheid, a student in Nussenzweig's lab,
isolated it several years ago
from an HIV - infected patient whose immune system had an exceptional ability to neutralize HIV in the
blood by preventing the virus
from infecting and destroying a specific type of immune cells, called CD4 cells, in patients.
Berkeley researchers
isolated circulating tumor cells
from the
blood of breast cancer patients, then used microscale physics to design a precision test for protein biomarkers, which are indicators of cancer.
So my field is science and technology, so my work is circulating tumor cells where I
isolate cancer cells
from blood of patients and
isolate their DNA and RNA and try to sequence them and try to device treatments for cancer treatment and therapy.
To deliver the treatment, doctors remove
blood from the patient and
isolate CD4 and other white
blood cells.
Using
blood samples
from a total of 210 patients, the scientists
isolated the genetic information and compared it with a control group of healthy persons.
At the same time, the KLS Foundation is working with laboratories to collect
blood samples
from KLS patients and their families for a genetic study, and nasal swabs
from patients during active episodes with the aim of
isolating an infectious trigger.
The cells were mixed with platelet - dense plasma
isolated from the patients»
blood and injected into their eyes.
Also on the horizon is the commercialization of cell - based tests with the use of cells
isolated from maternal
blood; this will create an even more competitive environment and will require greater diligence for proper validation, implementation, and patient education.