Sentences with phrase «isolated gene sequences»

Given the similarities between the Canadian Patent Act and its U.S. counterpart for the definition of «invention», the Canadian Patent Office has for many years granted claims like Myriad's to isolated gene sequences and continues to do so.

To date, no Canadian court has questioned the patentability of isolated gene sequences.

Not exact matches

Here's why: In the late 90s, technology emerged enabling researchers to rapidly sequence RNA and DNA — giving them the ability to isolate and record the activity of single genes.

Meanwhile, technology limits the impact of the Supreme Court ruling: The falling price of whole - genome sequencing, which sidesteps patents altogether because it doesn't require isolating a gene, makes it a reasonable alternative to a patented BRCA - style test.

Since the 1970s, scientists have been isolating proteins and laboriously determining their gene sequences.

Molecular geneticist Cheng Chi Lee, developmental biologist Gregor Eichele, and their co-workers at the Baylor College of Medicine in Houston have isolated a gene in mice and humans that shares 44 % of the amino acid sequence of the period (per) gene of the fruit fly Drosophila melanogaster.

Genome sequencing revealed that the isolated B. pumilus contained three unique gene clusters for the production of antimicrobial peptide compounds known as bacteriocins.

A family of neuropeptide genes which includes the gene encoding ELH along with two additional genes encoding the A and B peptides thought to initiate the egg - laying process has been isolated and their nucleotide sequence has been determined.

To narrow down the suspects in the US Airways crash, the Smithsonian lab's Carla Dove isolated and sequenced a small piece of a mitochondrial gene — known in the field as a DNA bar code.

First, samples of leaves from these plants are collected for in vitro cultures to isolate the fungi; then the DNA and RNA of fungi are extracted to sequence them and, through bioinformatic analysis, the researcher can determine the expression, the presence or absence of genes in the genomes of a species against each other.

The researchers isolated and sequenced DNA from the mouse droppings, studying a gene often used to distinguish bacterial species to find out what gut bugs were present.

In response to growing criticism about the lack of access to influenza data, the Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia, on 22 August made public the sequence of more than 650 genes from flu viruses isolated in the United States.

Wall then isolated those fungi and sequenced the gene FKBP12 — the target of FK506 — to look for mutations that would confer drug resistance.

The sequence had to be easy to isolate but couldn't jump inexplicably from one organism to another, as some genes do.

Thus µSCALE empties the contents of a single capillary onto a collector plate, where the DNA of the isolated cell can be sequenced and the gene variant responsible for the protein of interest can be identified.

By isolating strands of complementary DNA (cDNA) that fit together with mRNA, researchers can figure out what sequence was just translated into mRNA — that is, they can get a read on an expressed gene.

Much of the work to isolate the PHS gene came from Gill and his colleagues» efforts to fully sequence the genome — think genetic blueprint — of common wheat.

Here we use semi-conserved regions within short var gene sequence «tags» to make direct comparisons of var gene expression in 12 clinical parasite isolates from Kenyan children.

We are using a new technique, called single cell RNA sequencing, to isolate thousands of single neurons from human brain tissue, study all the genes that are expressed in each individual cell, and make cell - to - cell comparisons between normal, early stage and late stage AD.

By sequencing the exomes of multiple individuals, isolating what we'd call «tier 1» variants — Nonsynonymous, nonsense, splice site, or frameshift - indel — and then removing all known common variants from public databases, Dr. Shendure and colleagues can reduce 20,000 gene candidates down to a handful.

In principle, some of these limitations can be overcome by applying high - throughput sequencing to the study of introgression between partially - isolated populations that are characterized by a long history of extensive gene flow.

Fitness -, virulence - associated genome regions, and CRISPR / CAS (clustered regularly interspaced short palindromic repeats / CRISPR associated sequence) arrays of these STEC O156: H25 and O182: H25 isolates were highly similar, and identical genomic integration sites for the stx converting bacteriophages and the core LEE, identical Shiga toxin converting bacteriophage genes for stx1a, identical complete LEE loci, and identical sets of chemotaxis and flagellar genes were identified.

Purified isolates were identified by 16S rRNA gene sequencing and were subjected to antibiotic susceptibility profiling.

To this end, we determined the susceptibility of 38 NAPCR1 isolates to ten antibiotics from seven classes using E-tests or macrodilution tests and examined 31 NAPCR1 whole - genome sequences to identify single nucleotide polymorphisms and genes that could explain the observed resistance phenotypes.

Here, we report the genome sequence of this pathogen and 7,863 identified protein - coding genes, and we show that the majority of clinical isolates possess high levels of allelic diversity, genetic admixture, heterozygosity, and extensive aneuploidy.

The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data.

Viral gene sequences from an enlarged set of about 200 Epstein - Barr virus (EBV) strains including many primary isolates have been used to investigate variation in key viral genetic regions, particularly LMP1, Zp, gp350, EBNA1 and the BART miRNA cluster 2.

We performed targeted next - generation sequencing for 120 genes associated with myeloid neoplasms on megakaryocytes isolated from aspirated bone marrow.

Together, these improvements may permit real - time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates.

The immunopurification (IP) of Argonaute (Ago), a central component of the RISC in the human and mouse, followed by microarray analyses (Ago IP / microarray method) makes it possible to isolate any Ago - associated miRNAs and mRNAs without relying on the mechanism of regulation (i.e. mRNA decay or translational suppression), or sequence conservation, enabling a comprehensive identification of the miRNA - target genes in an unbiased manner.

With the canine genome now fully mapped and sequenced, the stage is set for mapping and isolating the genes responsible for many genetic disorders.