Sentences with phrase «isotype m»

However, these were all of the antibody isotype M (immunoglobulin M, IgM), the earliest antibody type elicited during an acute immune response.

CD8αα and - αβ isotypes are equally recruited to the immunological synapse through their ability to bind to MHC class I. EMBO reports.

This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

Not exact matches

«Linkers are stuck in the nanoparticle membrane and bind to a fixed region of any antibody of the same isotype.

By contrast, the anti-GalC antibodies in the GBS patients were of the isotype IgG.

Primary isolates that were incubated with identical concentrations of FITC - and PE - conjugated isotype standards were used to assess the level of background intensity.

HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

An isotype control for the secondary antibody was used as a negative control in both samples.

The secondary antibody must be specific to the isotype class of the primary antibody (e.g., IgG or IgM)

Therefore, it is critical to use a secondary antibody that specifically recognizes that isotype.

Therefore, to maximize detection of the target, it is best to use a secondary antibody that recognizes all isotypes.

After washing with PBS and blocking for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K isotype control (diluted 1:150; eBiosicence) for one hour.

Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.

Unstained, isotype control, and E-cadherin (E-cad)- stained cells are shown in the histogram.

Rat anti-mouse ST2 - blocking Ab and mouse IgG1 isotype control Ab were provided by Amgen.

An isotype control IgG was run in parallel and showed no positive staining (not shown here).

Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg / 1x106 cells) used under the same conditions.

LCMV - infected mice were injected i.p. with 150 μg ST2 blocking Ab or isotype control every other day beginning on day 2 postinfection (14).

Rat anti-mouse IFN - γ (XMG1.2, 0.5 mg), rat anti-mouse TNF - α (XT3.11, 1 mg), and rat IgG2a isotype control (2A3, 0.5 mg) were injected i.p. every third day beginning on day 2 postinfection (8).

(E) Mice were treated beginning day 2 postinfection, and every 3 d thereafter with isotype control, IFN - γ blockade only, TNF - α blockade only, or IFN - γ / TNF - α dual blockade following infection.

(a) Top panel: Unstained, isotype control, and ESA - stained cells are shown in the histogram.

Prf1 − / − mice were immunized against gp33 or with control procedure, rested for 30 d, infected with LCMV, and treated with either IFN - γ blockade or isotype control beginning day 2 postinfection, and every 3 d thereafter.

(B) gp33 - immunized and control mice were treated with IFN - γ blockade or isotype control Ab treatment.

The reactivity of this monoclonal antibody was not dependent on the V (L) or C (L) isotype of the fibril, but rather seemed to be directed toward a beta - pleated sheet conformational epitope expressed by AL and other amyloid proteins.

They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.

Cells were stained with STAT6 monoclonal antibody or mouse IgG1 isotype control antibody or without the primary antibody.

Quadrants were set based on isotype - stained controls.

Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).

An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.

An Alexa Fluor ® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.

Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated with trastuzumab, isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.

There are many different immunoglobulin isotypes and without L - aspartic acid many of these compounds can not be synthesised.

Included are representations of artworks and museum exhibits created by artists Oliver Ressler, Sayler / Morris, Dread Scott, Temporary Services, and others, original Isotype graphics drawn from the Museum's lexicon of «capitalisms,» and texts from Lucy Lippard, Lester K. Spence, T.J. Demos, Chantal Mouffe, McKenzie Wark and Kim Stanley Robinson, among others.

But once the isotype batteries are used up they will be disposed of only through authorized agents of the manufacturer.