Sentences with phrase «isotype control»
An "isotype control" is a substance used in scientific experiments to ensure accurate results. It acts as a comparison to separate the actual effect of the substance being tested from any non-specific reactions. It helps scientists identify the real changes caused by the substance under investigation. Full definition
Rat anti-mouse ST2 - blocking Ab and mouse IgG1 isotype control Ab were provided by Amgen.
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They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
Red histogram represents equal quantity of isotype control.
Rat anti-mouse IFN - γ (XMG1.2, 0.5 mg), rat anti-mouse TNF - α (XT3.11, 1 mg), and rat IgG2a isotype control (2A3, 0.5 mg) were injected i.p. every third day beginning on day 2 postinfection (8).
Corresponding isotype control, mouse IgG ‐ APC (R&D, #IC002A, 1:10) rat IgG - PE (ebioscience, # 12-4301-81, 1:100), was used.
After washing with PBS and blocking for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K isotype control (diluted 1:150; eBiosicence) for one hour.
(E) Mice were treated beginning day 2 postinfection, and every 3 d thereafter with isotype control, IFN - γ blockade only, TNF - α blockade only, or IFN - γ / TNF - α dual blockade following infection.
Prf1 − / − mice were immunized against gp33 or with control procedure, rested for 30 d, infected with LCMV, and treated with either IFN - γ blockade or isotype control beginning day 2 postinfection, and every 3 d thereafter.
A non-specific isotype control antibody (human IgG1) showed no reactivity.
(A) Survival of gp33 - immunized mice with LCMV infection following 30 d rest period compared with control mice with IFN - γ blockade or isotype control Ab treatment.
Flow cytometric analysis (A) of MCF7, MCF7 / HER2, and BT474 cells labeled with F3 - IgG, trastuzumab, or a human IgG isotype control antibody.
Use negative controls (unstained and isotype control antibodies) to determine gating of populations.
Negative staining and isotype controls are included to assess antibody staining relative to background during FACS analysis.
Isotype control antibody (black line) was mouse IgG2b [PLPV219](ab91366, 2µg / 1x106 cells) used under the same conditions.
HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
An isotype control for the secondary antibody was used as a negative control in both samples.
Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Isotype control antibody (black line) was rabbit IgG (monoclonal)(1µg / 1x10 ^ 6 cells) used under the same conditions.
Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg / 1x106 cells) used under the same conditions.
Unstained, isotype control, and E-cadherin (E-cad)- stained cells are shown in the histogram.
(C) Survival of gp33 - immunized mice with LCMV infection following 30 d rest period compared with control mice with ST2 blockade or isotype control Ab treatment.
LCMV - infected mice were injected i.p. with 150 μg ST2 blocking Ab or isotype control every other day beginning on day 2 postinfection (14).
(a) Top panel: Unstained, isotype control, and ESA - stained cells are shown in the histogram.
(B) gp33 - immunized and control mice were treated with IFN - γ blockade or isotype control Ab treatment.
All other neutralizing and isotype control Abs were obtained from Bio X Cell.
The black histograms in (C, D) represent the isotype controls.
The isotype control at 1 ug / mL shows no higher signal than the no primary negative control.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or isotype control antibody.