Sentences with phrase «isotype control antibody»
A non-specific isotype control antibody (human IgG1) showed no reactivity.
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Flow cytometric analysis (A) of MCF7, MCF7 / HER2, and BT474 cells labeled with F3 - IgG, trastuzumab, or a human IgG isotype control antibody.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.
Use negative controls (unstained and isotype control antibodies) to determine gating of populations.
Isotype control antibody (black line) was mouse IgG2b [PLPV219](ab91366, 2µg / 1x106 cells) used under the same conditions.
HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Isotype control antibody (black line) was rabbit IgG (monoclonal)(1µg / 1x10 ^ 6 cells) used under the same conditions.
Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg / 1x106 cells) used under the same conditions.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or isotype control antibody.