They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate
isotype control for 15 min in the dark at 4 °C.
An isotype control for the secondary antibody was used as a negative control in both samples.
Not exact matches
After washing with PBS and blocking
for 30 minutes (IHC / ICC Blocking Buffer - Low Protein; eBioscience), cells were incubated with anti - Oct ‑ 4 antibody (diluted 1:150; eBiosicence) or an IgG2a K
isotype control (diluted 1:150; eBiosicence)
for one hour.
Prf1 − / − mice were immunized against gp33 or with
control procedure, rested
for 30 d, infected with LCMV, and treated with either IFN - γ blockade or
isotype control beginning day 2 postinfection, and every 3 d thereafter.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated
for 6 days with F3 - IgG, trastuzumab, or
isotype control antibody.