The reactivity of this monoclonal antibody was not dependent on the V (L) or C (L)
isotype of the fibril, but rather seemed to be directed toward a beta - pleated sheet conformational epitope expressed by AL and other amyloid proteins.
A monoclonal antibody contains a single
isotype of immunoglobulin.
In addition, rabbits only have one
isotype of IgG molecules with more disulphide bonds.
A polyclonal antibody contains a mixture of several
isotypes of immunoglobulin G (e.g., IgG1, IgG2a).
ADCC / ADCP - enabling
isotypes of a-TIGIT surrogate Ab show potent monotherapy efficacy in murine tumor models that correlates to increased T cell activation and reduced Treg infiltration of tumors.
Not exact matches
«Linkers are stuck in the nanoparticle membrane and bind to a fixed region
of any antibody
of the same
isotype.
By contrast, the anti-GalC antibodies in the GBS patients were
of the
isotype IgG.
However, these were all
of the antibody
isotype M (immunoglobulin M, IgM), the earliest antibody type elicited during an acute immune response.
Use negative controls (unstained and
isotype control antibodies) to determine gating
of populations.
Primary isolates that were incubated with identical concentrations
of FITC - and PE - conjugated
isotype standards were used to assess the level
of background intensity.
AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting
of the neuron - specific class III b - tubulin
isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».
You will find a wide variety
of isotypes, species and conjugates in our website, such as gold, biotin, Dylight and Alexa Fluor ® dyes.
HL - 60 cells were stained with 1 µg / mL ab14715 (blue) or an equal amount
of an
isotype control antibody (red) and analyzed by flow cytometry.
The secondary antibody must be specific to the
isotype class
of the primary antibody (e.g., IgG or IgM)
(d) Histogram
of membrane - bound Tie2 expression in HUVEC and BP compared to
isotype control measured by flow cytometry.
Therefore, to maximize detection
of the target, it is best to use a secondary antibody that recognizes all
isotypes.
(C) Survival
of gp33 - immunized mice with LCMV infection following 30 d rest period compared with control mice with ST2 blockade or
isotype control Ab treatment.
They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution
of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate
isotype control for 15 min in the dark at 4 °C.
Results : The application
of specific optimised conditions induce multiple rounds
of memory B cell proliferation equally across Ig
isotypes, differentiation
of memory B cells to antibody secreting cells, and importantly do not alter the Ig genotype
of the stimulated cells.
Inhibition
of cell proliferation (B)
of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or
isotype control antibody.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL
of the IgG1
Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L)
of IgG.
To assess the ability
of F3 - IgG to inhibit the proliferation
of HER2 - overexpressing breast carcinoma cells, we measured the proliferation
of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence
of trastuzumab, F3 - IgG, or an
isotype - matched control antibody.
There are many different immunoglobulin
isotypes and without L - aspartic acid many
of these compounds can not be synthesised.
Included are representations
of artworks and museum exhibits created by artists Oliver Ressler, Sayler / Morris, Dread Scott, Temporary Services, and others, original
Isotype graphics drawn from the Museum's lexicon
of «capitalisms,» and texts from Lucy Lippard, Lester K. Spence, T.J. Demos, Chantal Mouffe, McKenzie Wark and Kim Stanley Robinson, among others.
But once the
isotype batteries are used up they will be disposed
of only through authorized agents
of the manufacturer.
Guanacaste: shore
of the Gulf
of Nicoya, sea level, Feb. 1900, A. Tonduz13538 (lectotype, designated here, US [B destroyed];
isotypes, BM, GH, K, NY, US).