Not exact matches
AHERF Neurosciences Grant (G9410 -020-Y01)- Co-PI; «Differential expression and sorting of the neuron -
specific class III b - tubulin
isotype in human neuronal and neuroendocrine tumor cell lines maintained in an organ culture system».
The secondary antibody must be
specific to the
isotype class of the primary antibody (e.g., IgG or IgM)
Results : The application of
specific optimised conditions induce multiple rounds of memory B cell proliferation equally across Ig
isotypes, differentiation of memory B cells to antibody secreting cells, and importantly do not alter the Ig genotype of the stimulated cells.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1
Isotype Control Antibody and detecting with Alexa Fluor conjugates
specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.