Hindlimb bones were excised, fixed in 10 % neutral - buffered formalin, and imaged using a μCT scanner (SkyScan 1176, Bruker Corp.) at 17 - μm resolution and 180 ° scanning with a rotation step of 0.7 ° per image, 242 - ms exposure time, 55 -
kV photon energy, and 455 - μA current.