Sentences with phrase «kahweol concentrations»

C) Plasminogen zymography of HUVEC uPA after treatment with different kahweol concentrations.

The kahweol concentrations required to inhibit the differentiation of HUVEC cells did not affect their viability (results not shown).

A) Gelatin zymography of MMP - 2 in conditioned media of HUVEC after treatment with different kahweol concentrations.

In all these in vitro assays, kahweol treatments were carried out under conditions (kahweol concentration and duration of treatment) that did produce no cytotoxic effect on cells.

Not exact matches

The minimal inhibitory concentration for kahweol in this assay of «tubule - like» structures formation on Matrigel was 25 µM, in the range of concentrations at which other known antiangiogenic compounds produce this kind of effect [13], [27].

C) Quantification of the amount of MCP - 1 secreted by HUVEC after a 24 h treatment in the presence of different concentrations of kahweol.

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Subconfluent HUVEC cultures were stimulated with PMA (50 ng / mL) for 4 h in the absence (controls) or presence of different concentrations of kahweol.