Sentences with phrase «kahweol in»

B) In situ determination of kahweol effects on HT - 1080 gelatinases, as determined by gelatin zymography with the presence of kahweol in the incubation substrate buffer.

The minimal inhibitory concentration for kahweol in this assay of «tubule - like» structures formation on Matrigel was 25 µM, in the range of concentrations at which other known antiangiogenic compounds produce this kind of effect [13], [27].

Not exact matches

Furthermore, a clear dose - response inhibition of microvessel formation was observed and quantified in treatments with kahweol.

In contrast, Video S2 shows that larvae treated with 50 µM kahweol for 24 h exhibited no blood flow along intersegmental vessels.

A) Gelatin zymography of MMP - 2 in conditioned media of HUVEC after treatment with different kahweol concentrations.

To get new, additional insights on the features of kahweol as an anti-angiogenic compound, we carried out a complete set of in vitro assays previously used by us to characterize the anti-angiogenic effects of other compounds from natural sources, including aeroplysinin - 1, homocysteine, ursolic acid, puupehenone, hypericin, hyperforin and aloe - emodin, among others [11], [12], [13], [19], [20], [21].

In the CAM assay, the inhibitory doses exhibited by kahweol are similar to those of other anti-angiogenic compounds found by us to inhibit angiogenesis in the CAM assay [10], [19], and much lower than those of other anti-angiogenic compounds [21In the CAM assay, the inhibitory doses exhibited by kahweol are similar to those of other anti-angiogenic compounds found by us to inhibit angiogenesis in the CAM assay [10], [19], and much lower than those of other anti-angiogenic compounds [21in the CAM assay [10], [19], and much lower than those of other anti-angiogenic compounds [21].

The global morphological features (including centrifugal growth of the peripheral vessels - relative to the position of the disc -, avoiding the treated area, with an overall decrease in the vascular density) elicited by kahweol treatment are also in agreement with those previously observed for other anti-angiogenic compounds.

Altogether, our results demonstrate that kahweol is a potent anti-angiogenic compound both in vitro and in vivo, targeting some key steps shared with tumor progression, key molecules involved in ECM remodeling (MMP - 2 and uPA), and key molecules involved in inflammation (COX - 2 and MCP - 1).

In all these in vitro assays, kahweol treatments were carried out under conditions (kahweol concentration and duration of treatment) that did produce no cytotoxic effect on cellIn all these in vitro assays, kahweol treatments were carried out under conditions (kahweol concentration and duration of treatment) that did produce no cytotoxic effect on cellin vitro assays, kahweol treatments were carried out under conditions (kahweol concentration and duration of treatment) that did produce no cytotoxic effect on cells.

On the other hand, the present research work shows a confirmatory evidence of the potential of kahweol to inhibit in vivo angiogenesis, by using another completely independent model system, namely, that of genetically modified zebrafish.

Taken together, our data indicate that, indeed, kahweol behaves as an anti-inflammatory and anti-angiogenic compound with potential use in antitumoral therapies.

Figure 8 (C) shows that kahweol - treatment induces a dose - dependent decrease in the levels of urokinase in HUVEC conditioned media, with an almost complete inhibition at 50 µM kahweol.

Different in vitro assays were carried out in order to test the specific effects of kahweol treatment on several key steps of the angiogenic process in both endothelial and tumor cells.

The CAM and zebrafish in vivo assays and the ex vivo mouse aortic ring assay clearly identify kahweol as a new anti-angiogenic compound, but gives no information on which specific steps of angiogenesis are targeted by this compound.

Figure 2 (middle line) shows that 50 nmol kahweol induced apoptosis in a small percentage of cells but this effect did not seem to be endothelial cell specific, since only few apoptotic nuclei corresponded to endothelial cells.

One of these is kahweol (Figure 1), an antioxidant diterpene that remains in unfiltered coffee beverages, such as Turkish and Scandinavian coffee [3].

Video images of blood flow thru intersegmental vessels were taken on the caudal region next to vitellus in 48 h larvae after 24 h of treatment in the absence (control, Video S1) or presence (Video S2) of 50 µM kahweol.

We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP - 2 and uPA.

Figure 9 (A and B) shows that kahweol inhibits in a dose dependent manner the expression of COX - 2 protein by HUVEC.

C) Quantification of the amount of MCP - 1 secreted by HUVEC after a 24 h treatment in the presence of different concentrations of kahweol.

Data obtained on the effects of kahweol on endothelial cell invasion (as determined by a continuous fluorescent assay) clearly show that kahweol induces an anti-invasive effect in HUVEC in a dose - dependent manner (Figure 7).

Therefore, kahweol - treatment has another key target in this essential step of the angiogenesis process.

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Subconfluent HUVEC cultures were stimulated with PMA (50 ng / mL) for 4 h in the absence (controls) or presence of different concentrations of kahweol.

A) Negative of photographs (x20) of aortic rings (lateral view) after 10 days of incubation in a 3D collagen gel overlayed with complete medium in the presence of 20 mg / mL VEGF, 0.05 % DMSO (the vehicle taken as a control), or kahweol at 1, 5 and 25 µM (K1, K5, K25, respectively).

Although 50 nmol of kahweol per CAM was required to observe in vivo inhibition of angiogenesis in 100 % of treated eggs, as little as 10 nmol of kahweol was enough to induce clear inhibition of angiogenesis in 25 % of the tested eggs in the CAM assay.

We show for the first time that kahweol is an anti-angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation, migration, invasion and tube formation on Matrigel.

Our results in the zymographic assays for gelatinase and urokinase activities clearly showed that, in fact, kahweol was able to inhibit the expression of both MMP - 2 and uPA, identifying them as two relevant molecular targets for kahweol.

It has been shown previously that kahweol exerts a suppressive effect on COX - 2 expression in macrophages [38].

Diterpenes such as cafestol and kahweol are both present in the oil contained within the coffee bean, and these can increase your total cholesterol and LDL levels.

Medicinal compounds detected in coffee include lignans, quinides, antioxidants, dipertenes like kahweol and cafestol...

Research on the antioxidant activity of trigonelline, cafestol and kahweol has been less extensively investigated in humans.

Other compounds found in coffee include phenol chlorogenic acid (an antioxidant), potassium, magnesium, niacin (vitamin B1), kahweol, and cafestol.