Sentences with phrase «kahweol treatment»

This effect on cell survival was not endothelial cell - specific, since IC50 values for kahweol treatment of several human tumoral cell lines were similar to those obtained for HUVEC (results not shown).

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Different in vitro assays were carried out in order to test the specific effects of kahweol treatment on several key steps of the angiogenic process in both endothelial and tumor cells.

The global morphological features (including centrifugal growth of the peripheral vessels - relative to the position of the disc -, avoiding the treated area, with an overall decrease in the vascular density) elicited by kahweol treatment are also in agreement with those previously observed for other anti-angiogenic compounds.

In all these in vitro assays, kahweol treatments were carried out under conditions (kahweol concentration and duration of treatment) that did produce no cytotoxic effect on cells.

Not exact matches

Furthermore, a clear dose - response inhibition of microvessel formation was observed and quantified in treatments with kahweol.

Figure 6 shows the effects of 75 µM kahweol on endothelial cell migration, as determined by the «wound healing» assay, after 8 and 24 h of treatment.

A) Gelatin zymography of MMP - 2 in conditioned media of HUVEC after treatment with different kahweol concentrations.

Figure 8 (C) shows that kahweol - treatment induces a dose - dependent decrease in the levels of urokinase in HUVEC conditioned media, with an almost complete inhibition at 50 µM kahweol.

Video images of blood flow thru intersegmental vessels were taken on the caudal region next to vitellus in 48 h larvae after 24 h of treatment in the absence (control, Video S1) or presence (Video S2) of 50 µM kahweol.

C) Quantification of the amount of MCP - 1 secreted by HUVEC after a 24 h treatment in the presence of different concentrations of kahweol.

Therefore, kahweol - treatment has another key target in this essential step of the angiogenesis process.

C) Plasminogen zymography of HUVEC uPA after treatment with different kahweol concentrations.