Sentences with phrase «kilobase fragments»

The authors of this study have developed a universal primer set across flowering plants that amplifies 3 - 15 kilobase fragments, which can then easily be sequenced using recently developed next - generation sequencing technologies.

Not exact matches

Long - range PCR is a method that allows for the amplification of much larger fragments of DNA than is possible with traditional PCR — fragments larger than 40 kilobases have been reported in long PCR, versus fewer than 10 kilobases for traditional PCR.

The wt allele yields a 6.5 - kilobase (kb) fragment, whereas both targeted Xpd † XP and Xpd † XPCS alleles yield a 5.1 - kb fragment.

Gene - level expression values were represented by fragments per kilobase exon per million reads mapped (FPKM) and a consensus FPKM was determined for each gene based on its representation across biological replicates.

To compare the expression values across genes and conditions, raw count data was transformed into fragments per kilobase of exon per million fragments (FPKM) with the formula:

First, they sheared DNA into fragments that were a few kilobases long, ligated adapters to the ends of each fragment, and did a round of amplification.