Sentences with phrase «kinase assay»

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

We can clearly observe RIPK2 inhibitor 1 and 2 inhibition of MDP - dependent activation of RIPK2 autophosphorylation (on tyrosine 474) using an in vitro kinase assay in HCT116 cells (Fig. 3B).

(Top) An in vitro kinase assay was carried out by IP overnight with 1 μg of the rabbit anti-RIPK2 antibody from ProteinTech (Rosemont, IL) and 1 ml of lysate from a confluent six - well dish of KMH2 cells.

(D) RIPK2 in vitro kinase assay was carried out as in Fig. 3B in KMH2 and HCT116 cells in the presence of ponatinib or RIPK2 inhibitor 1 to carry out a comparison of RIPK2 inhibition.

This concentration was used due to robust inhibition of NFκB and RIPK2 kinase assay in assays outlined in Figs. 2 — 6.

The manuscript also contains the results of screening each compound in PKIS2 against the DiscoverX panel of more than 400 kinase assays.

Not exact matches

The recent explosion of green fluorescent protein (GFP) based sensors of pH, halides, proteases, kinases, G proteins, and many second messengers (including Ca2 +, cAMP, cGMP, nitric oxide, and IP3) have provided important tools for the design of screening assays.

Rho - kinase inhibitor treatment increased the cellular proliferation up to twofold during the first 12 h, and a wound model based migration assay showed 50 % faster filling of the mechanically generated wound area.

Activity assays for extracellular signal - regulated kinase 5.

To directly examine whether ABL kinases regulate tumor - induced osteoclast activation, we used an in vitro osteoclastogenesis assay (Fig. 5A).

Phos - tag analysis of Rab10 phosphorylation by LRRK2: a powerful assay for assessing kinase function and inhibitors

Abbreviations: ACVR2A, activin A receptor type IIA; BMP, bone morphogenetic protein; BMPR, BMP receptor, type II; CNS, Central nervous system; DA, dopaminergic; DMEM / F12, Dulbecco's modified Eagle's medium nutrient mixture F - 12; E, embryonic day; GDF, growth differentiation factor; GO, gene ontology; KEGG, Kyoto encylopedia of genes and genomes; MAPK, Mitogen - activated protein kinase; mDA, midbrain dopaminergic; PD, Parkinson's disease; RIPA, radioimmunoprecipitation assay; SN, Substantia nigra; TGF - β, transforming growth factor - β; TH, tyrosine hydroxylase; VM, ventral midbrain / mesencephalon; Zeb2, Zinc finger E-box-binding homoeobox 2

(C) RIPK2 in HCT116 cells were IP with an anti-RIPK2 antibody (A) and the supernatant after IP in (A) was IP in (B) with the same RIPK2 antibody, and in vitro kinase (IVK) assay was carried out on both samples.

This bound to the ATP binding pocket of the kinase domain and inhibited RIPK2 with an IC50 of 5 — 50 nM, depending on the assay.

The assay identifies both internal tandem duplication (ITD) and tyrosine kinase domain (TKD) mutations, and identifies even large ITD mutations, missed using many current NGS - based assays.

Amplified Luminescent Proximity Homogeneous Assay Screen (AlphaScreen) AlphaScreen is a versatile assay technology developed to measure analytes using a homogenous protocol that enables sensitive and precise interrogation of signaling pathways, receptors, enzymes and kinase targets, in a cell - based foAssay Screen (AlphaScreen) AlphaScreen is a versatile assay technology developed to measure analytes using a homogenous protocol that enables sensitive and precise interrogation of signaling pathways, receptors, enzymes and kinase targets, in a cell - based foassay technology developed to measure analytes using a homogenous protocol that enables sensitive and precise interrogation of signaling pathways, receptors, enzymes and kinase targets, in a cell - based format.

We combine biochemical, structural, cellular and functional information using purified proteins, mutant and transgenic plants, yeast and chemical genomic screening systems, transient gene expression assays, confocal microscopy and in silico data analysis to compare ROP - centered kinase signaling during cell polarity (in vitro pollen tubes), morphogenesis (whole plant) and pathogenesis (fungi - infected cells).

Oversaw cell based assay development, Kinase receptor activation assay (KIRA), cell adhesion assays, reporter gene assays, cell bridging assays, and Cytotoxicity assays.

Screened the analogues for the inhibition of protein kinase C using 32P - radioactive assay and found some interesting analogues with outstanding inhibition activity.