Sentences with phrase «knockout mice on»

«Because BAI1 is very active in the hippocampus, a region of the brain critical for learning and memory, we decided to look at the performance of the knockout mice on a memory task dependent on the hippocampus,» Van Meir says.

Not exact matches

Topical application of capsaicin on the dorsal skin of 7,12 - dimetylbenz (a) anthracene — initiated and TPA - promoted TRPV1 wild - type (WT) and TRPV1 knockout (KO) mice induced more and larger skin tumors in TRPV1 / KO mice, suggesting a TRPV1 - independent mechanism.

Some of these, based on previous studies of knockout mice, seem to play a role in cognition and behavior, including fear responses and the ability to learn new behaviors when given food rewards.

Zeroing in on this kinase was encouraging, Goga said, because other researchers have shown that genetic - knockout mice that lack the entire family of PIM kinases are slightly smaller than normal mice, but «basically fine,» indicating that a drug targeting just PIM1 may have manageable levels of toxicity in breast cancer patients.

The knockout mice also did better on tests of behaviors associated with the brain's prefrontal cortex, the area that regulates complex thinking, emotions, and behavior in humans.

On the other hand, as they aged, these «knockout mice» grew fatter than the normal mice, especially when fed a high - fat diet.

Here, we demonstrate that astrocytes are a direct insulin target in the brain and that knockout of IR on astrocytes causes increased anxiety and depressive - like behaviors in mice.

For more information on the Knockout Mouse Project, go to the NIH Knockout Mouse Project.

But the scientists found that the insulin - receptor knockout mice were on a fast track to atherosclerosis.

The 19 NIH institutes, centers and offices contributing to the Knockout Mouse Project are: the NIH Office of Strategic Coordination / Common Fund; NCRR; the National Eye Institute; NHGRI; the National Institute of Allergy and Infectious Diseases; the National Heart, Lung and Blood Institute; the National Institute on Aging; the National Institute of Alcohol Abuse and Alcoholism; the National Institute of Arthritis and Musculoskeletal and Skin Diseases; the Eunice Kennedy Shriver National Institute of Child Health and Human Development; NIDCD; the National Institute of Dental and Craniofacial Research; the National Institute of Environmental Health Sciences; the National Institute of General Medical Sciences; the National Institute of Mental Health; the National Institute of Neurological Disorders and Stroke; the National Institute of Diabetes and Digestive and Kidney Diseases; the National Cancer Institute; and the Office of AIDS Research.

«NIH is committed to making knockout mouse models more widely accessible to the biomedical research community,» said James Battey, M.D., Ph.D., director of the National Institute on Deafness and Other Communication Disorders (NIDCD), who is also the co-chair of the Trans - NIH Mouse Initiamouse models more widely accessible to the biomedical research community,» said James Battey, M.D., Ph.D., director of the National Institute on Deafness and Other Communication Disorders (NIDCD), who is also the co-chair of the Trans - NIH Mouse InitiaMouse Initiative.

Once each knockout mouse is phenotyped, researchers can obtain information on what knockout mouse lines are available and how to order them from the University of California Davis KOMP Repository.

A researcher with access to a low - cost knockout mouse that has been extensively phenotyped can focus his or her time and research budget on more in - depth research questions rather than spending it on producing a knockout mouse about which the researcher has limited information.

The process used by NIH to select the mouse lines involved a rigorous scientific review process that evaluated information on the knocked out gene, the reliability of the method used to produce the knockout, and whether the mouse line possesses a «reporter» gene, which enables researchers to analyze the pattern of the knockout gene's expression in various mouse tissues.

For more information on what knockout mice are, how they are made and what they are used for, go to Knockoknockout mice are, how they are made and what they are used for, go to Knockout Mmice are, how they are made and what they are used for, go to KnockoutKnockout MiceMice.

In a series of related studies, the scientists wanted to see what impact vitamin A would have on «knockout mice» missing a gene called sonic hedgehog or Shh.

Effects of muscarinic receptor antagonists on cocaine discrimination in wild - type mice and in muscarinic receptor M1, M2, and M4 receptor knockout mice Joseph L, Thomsen M. Behavioural Brain Research.

The IMPC builds on the efforts of IKMC to produce knockout mice and carry out high - throughput phenotyping of each line in order to determine the function of every gene in the mouse genome.

These results contrast with traditional knockout mice, where compensatory mechanisms mean that knockout of either PDS95 or PDS93 have no effect on synaptic transmission (Elias 2006).

The animal house and animal facilities at the CNB - CSIC have been registered at the Ministry of Agriculture, Fisheries and Food, regarding the use of animals in experimentation, with nº 28079 -29-A; and at the Ministry for the Environment, through the National Commission on Biosafety, regarding the experiments with genetically modified mice (both transgenic and knockout) under risk type II, with nº A / ES / 03 / I - 5.

WFSM establishes laboratory to develop transgenic and knockout mice to model human disease and shed light on function of human genes.

Using this treatment scheme, we observed a robust difference on day 9 in the disease activity indices of wild - type and Rassf1a knockout mice that was significantly inhibited with the use of the RIPK2 inhibitors, especially RIPK2 inhibitor 1 (Fig. 6B; Table 2).

Likewise, no effect on dopaminergic cell numbers was observed when DJ - 1 / parkin knockout mice were crossed with mice deficient for either SOD1 or SOD2 and aged to at least 16 months (Hennis et al., 2013).

The Comparative Mouse Genomics Centers Consortium (CMGCC) was initiated by the National Institute of Environmental Health Sciences» (NIEHS) Environmental Genome Project to develop transgenic and knockout mouse models based on human DNA sequence variants in environmentally responsive gMouse Genomics Centers Consortium (CMGCC) was initiated by the National Institute of Environmental Health Sciences» (NIEHS) Environmental Genome Project to develop transgenic and knockout mouse models based on human DNA sequence variants in environmentally responsive gmouse models based on human DNA sequence variants in environmentally responsive genes.

Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) in DMEM / F12 culture medium supplemented with KnockOut serum replacer (20 %), non-essential amino acids (0.1 mM), L - glutamine (1 mM), ß - mercaptoethanol (0.1 mM) and 100 ng / ml zebrafish basic fibroblast growth factor.

EMMA strains produced from ES cells generated by the International Knockout Mouse Consortium (IKMC) are displayed on the EMMA strain list as soon as germ - line transmission has been achieved and the genotype of the pups has been confirmed.

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

On October 5, 2005, NIH announced that contracts had been signed with two companies, Deltagen, Inc. and Lexicon Genetics, Inc., to provide knockout mouse lines and extensive phenotyping data on them to NIOn October 5, 2005, NIH announced that contracts had been signed with two companies, Deltagen, Inc. and Lexicon Genetics, Inc., to provide knockout mouse lines and extensive phenotyping data on them to NIon them to NIH.

Body composition was unaltered in Lyplal1 knockout mice as assessed by Dual - energy X-ray absorptiometry (DEXA) scanning, both on normal chow and on a high fat diet.

On October 5, 2005, NIH announced that contracts had been signed with two companies, Deltagen, Inc. and Lexicon Genetics, Inc. (now Lexicon Pharmaceuticals), to provide knockout mouse lines and extensive phenotyping data on them to NIOn October 5, 2005, NIH announced that contracts had been signed with two companies, Deltagen, Inc. and Lexicon Genetics, Inc. (now Lexicon Pharmaceuticals), to provide knockout mouse lines and extensive phenotyping data on them to NIon them to NIH.

A role for the Ca2 + channel TRPML1 in gastric acid secretion, based on analysis of knockout mice.