The same principle lies behind the powerful and visually spectacular Brainbow cell - labeling technique, in which
each labeled cell expresses a unique ratio of three or four distinct colors of fluorescent protein.
Neighboring GFP -
labeled cells express NPF or SIFamide (S1 Fig).
Not exact matches
A radio -
labeled human antibody binds to the viral gp41 protein
expressed on the surface of the HIV - infected human lymphocyte and the
cell is killed by alpha radiation delivered by the antibody.
The group employed various viral tracing methods — infecting receptor -
expressing neurons with a virus strain and watching them spread as they
label infected
cells with a fluorescent protein — to visualize the neural circuit downstream of the ESP1 receptor, as well as providing an image of nerve fibers belonging to specific neurons in the brain and synapses relaying impulses from neuron to neuron, to map the anatomical foundation that conveys ESP1 signals in the brain.
The team went on to show that supplementing heavy stable isotope -
labeled forms of these precursors led to incorporation of heavy amino acids into proteins produced in enzyme
expressing cells.
They found that injecting into the carotid artery breast cancer
cells that
express markers allowing them to enter the brain —
cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.
The injected
cells express markers that allow them to enter the brain and are
labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies.
Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to
label cells stochastically using gene cassettes that code for four uniquely
expressed fluorescent proteins.
In contrast, EFTF -
expressing pluripotent
cells generated YFP -
labeled eyes in 63 % of transplants (N = 74).
Difficult - To -
Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental cond
Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The
Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are
labeled as «difficult - to -
express» proteins because of low titers and sensitivity to environmental cond
express» proteins because of low titers and sensitivity to environmental conditions.
One common complaint about using four - color Confetti to
label single
cells (in an animal) is that the
cells express green fluorescent protein less often, or sometimes not at all, compared with
cells that
express each of the other three colors.
Furthermore, enhanced hiPS - HEP
cells express high levels of the LDL receptor (Figure 5, Panel B) and take up fluorescently
labeled LDL (Figure 5, Panel A).
Although the yeast
cells in these micrographs are
expressing specific prion - like proteins (green,
expressed protein
label along top of micrographs), neither yeast containing prion - like proteins or the control exhibit the large protein masses expected in
cells expressing prions.
Tang wondered if he could use previously established mouse lines
expressing GFP in specific
cell types to do more than just
label cells.
To ascertain this we generated
cell lines that both contained the HV cassette and constitutively
expressed β - gal as a lineage
label.
The randomized, open
label Phase 2 trial is ongoing and evaluating CMB305 and atezolizumab in patients with locally advanced, relapsed, or metastatic synovial sarcoma or myxoid / round -
cell liposarcoma, two types of sarcoma that tend to
express NY - ESO - 1 broadly.