Oligonucleotide -
labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.
Not exact matches
Brilliant Violet dye conjugated secondary
antibodies from Jackson ImmunoResearch
allow the addition of more colors to multiple
labeling assays.
Labeled antibodies, which bind to specific cell types,
allowed the researchers to determine which types of neuron were being created at which times.
In the current study, the researchers used high - affinity
antibodies to «
label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which
allowed very detailed visualization at individual synapses, or gaps between nerve cells.
A few years later, Karl Deisseroth's lab developed CLARITY, which is a hydrogel - based method that also
allows antibody penetration for immunohistochemical
labeling in whole tissue (15).
Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for
labelling using complex ranges of
antibodies whose compatibility
allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific
antibodies and cell viability).
Flow cytometry is a powerful technique that
allows researchers to examine multiple proteins on cell populations using fluorescently
labeled antibodies.