Sentences with phrase «labeled by this antibody»

At 13 months of age, the intracellular MOAB - 2 immunolabel was hardly detectable in pyramidal neurons adjacent to amyloid plaques, the latter which were strongly labeled by this antibody and not by pab27576 (Additional file3: Figure S2 a-b).

Not exact matches

A radio - labeled human antibody binds to the viral gp41 protein expressed on the surface of the HIV - infected human lymphocyte and the cell is killed by alpha radiation delivered by the antibody.

Diego Grassi, PhD, a research associate in Lasmézas» lab, made this discovery by labeling the pα - syn * with an antibody so he could follow it throughout the cell after it was created.

The SVCs were incubated with a fluorescently labeled anti-F4 / 80 antibody and were sorted by FACS into F4 / 80 - expressing (F4 / 80 +) and - nonexpressing (F4 / 80 ---RRB- populations.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

(E) Dilp5 in the IPCs was detected by anti-Dilp5 antibody in wild - type and CCHa2 - R mutants, in which the IPCs were labeled by dilp2 - GAL4 driving UAS - mCD8:: GFP.

Immunofluorescent labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by fluorescently labeled, secondary anti-Ig antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.

Antibody binding was visualized by chemiluminescence detection in a CCD - camera system using a peroxidase (HRP) labeled secondary aAntibody binding was visualized by chemiluminescence detection in a CCD - camera system using a peroxidase (HRP) labeled secondary antibodyantibody.

The researchers used an antibody that labels the proteins made by two closely linked homeotic genes, Ubx and abdA, to show in which segments of the embryo the genes are turned on.

(C) Dilp2 in the IPCs was detected by Dilp2 antibody in wild - type and CCHa2 - R mutants in which the IPCs were labeled by dilp2 - GAL4 - driven UAS - mCD8:: GFP.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

In addition to staining the midgut contents with FITC - labeled polyclonal anti-Borrelia species antibody, we washed and re-stained this set and stained the second set of xenodiagnostic tick (XT) samples with an anti-OspA monoclonal antibody (CB10, obtained from J. Benach [41]-RRB-, followed by anti-mouse IgG - Alexa 488 (Molecular Probes).

By Day 42, immunized animals generated plasma Abeta antibodies that labeled Abeta plaques in human, AD transgenic mouse and vervet brains; bound Abeta1 - 7; and recognized monomeric and oligomeric Abeta but not full - length amyloid precursor protein nor its C - terminal fragments.

Cells were fixed, permeabilized and then labelled with ab14730 followed by an AlexaFluor ® 488 - conjugated Goat anti-Mouse IgG1 - specific secondary antibody (2 µg / ml)

For colocalization of both tracers or one tracer together with ZENK signals, primary antibodies (Egr - 1 / ZENK, 1 ∶ 500; CtB, 1 ∶ 300 in PBS - T) were detected by an appropriate secondary antibody (polyclonal goat raised against rabbit IgG labeled with fluorescent dyes Alexa488; Molecular Probes Europe BV, Leiden, The Netherlands, 1 ∶ 400 in PBS - T).

Such an optical contrast exceeded the optical contrast of the fluorescent labels (that were targeted to C4 - 2B and HS - 5 cells using the same prostate cancer - specific PSMA antibody, see Figure 7B, D) by 31 times.

By immunofluorescence microscopy, Thy1 - PE - labeled CD3ε + cells were observed in the SCS and in nearby lymphatic sinuses, and few labeled cells were detected within the LN parenchyma, confirming that footpad injection of PE - conjugated antibody led to preferential labeling of lymph - exposed LN cells (Figure 3E).