Sentences with phrase «labeled cell at»

Not exact matches

Meanwhile, researchers at Columbia University in New York are working on a way to add dozens of color labels to Raman - imaged cells.

Also look at the institution's broader scientific environment, because «you can benefit from help even from people that are not with the label «biomaterials,»» says Dupont - Gillain, who, as a bioengineer and physical chemist, collaborates with colleagues in surface science and cell biologists to develop biomimetic surfaces that can trigger desired cell behaviors.

Photo of a living Brainbow zebrafish, taken by Zachary Tobias (a research technician in Weissman - Unni's lab), showing a brightly labeled neuron with its cell body (white) at bottom.

Biologists at the University of Cambridge used fluorescent labelling to stain cell membranes in skate embryos, and tracked them through the dynamic development process.

To label individual cells, researchers use a very thin violet laser beam aimed at small blood vessels.

To better understand what happens during immune reactions in the body, researchers at Tübingen University have developed a new way of labeling T - cells, allowing them to track the T - cell movement in mice using non-invasive positron emission technology (PET).

«We showed that the presence of the maternal gut microbiota during late pregnancy blocked the passage of labeled antibodies from the circulation into the brain parenchyma of the growing fetus,» says first author Dr. Viorica Braniste at the Department of Microbiology, Tumor and Cell Biology at Karolinska Institutet.

By snapping 3D images of fluorescently labeled embryos, Kuroda's team found that in dextral snails, the spindles — tubular structures holding the chromosomes — are already spiraled and the cell boundaries seem twisted at an early stage of the third cleavage.

Labeled antibodies, which bind to specific cell types, allowed the researchers to determine which types of neuron were being created at which times.

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

The number of car models, where at least one variant has a combined city / highway label fuel economy of at least 30 mpg, has grown from 46 models in MY 2012 to more than 70 models in MY 2017, and the number of car models with 40 mpg or higher has more than doubled (comprised of hybrid, electric (EV), plug - in hybrid electric (PHEV), and fuel cell vehicles (FCV)-RRB-.

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldeCells were washed with label - free medium and fixed in 4 % paraformaldehyde.

It was at this stage that highly purified populations of endothelial cells could be obtained by adjusting the gating parameters to select only the most intensely labeled cells.

Cells treated with hamster anti-αβTCR or hamster murine γδTCR were washed three times in HBSS and incubated in PE - labeled Armenian hamster antihamster IgG (PharMingen) secondary antibody for 20 min at 4 °C, washed three additional times in PBS, fixed in 1 % paraformaldehyde, and assessed for fluorescence in a FACScan flow cytometer (Becton Dickinson).

«If you went back a few years and opened a textbook you'd see the classic hippocampal circuit that's been diagrammed for a hundred years, and you'd look at the subiculum and you'd see it labeled as one region, with one type of cell, doing one thing,» Cembrowski explains.

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» explained Christiansen, software engineer at Google Accelerated Science.

Researchers used this approach to label memory cells during a fear - conditioning event that involved a mild electric shock to the mouse and then used light to artificially reactive memories at different times.

Using colorfully labeled antibodies to light up the new protein under their microscopes, they found that it was present where Listeria entered cells and also present at adherens junctions between epithelial cells.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA.

Immunofluorescent analysis of HepG2 cells using 60008 -1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

«We trained the neural network by showing it two sets of matching images of the same cells; one unlabeled and one with fluorescent labels,» coauthor Eric Christiansen, a software engineer at Google Accelerated Science, says a press release.

In 2012, when Tonegawa Laboratory, a neuroscience research lab at MIT, developed a way to label engram cells, this opened the door to new ways to test memory consolidation theories.

Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004 -1-Ig (GAPDH antibody) at dilution of 1:50 and Rhodamine - labeled goat anti-mouse IgG (red).

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

At the end of the cell - isolation process, end users decouple the magnetic particles from target cells with a release buffer and clear magnetic label contaminants.

The SCiStar study is an open - label, single - arm trial testing three sequential escalating doses of AST - OPC1 administered at up to 20 million AST - OPC1 cells in 25 subjects with subacute motor complete (AIS - A or AIS - B) cervical (C - 4 to C - 7) spinal cord injury.

Oligonucleotide - labeled antibodies allow integration of cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed protein markers far surpassing what can be measured by cytometry - based approaches.

Researchers at Columbia University have made a significant step toward breaking the so - called «color barrier» of light microscopy for biological systems, allowing for much more comprehensive, system - wide labeling and imaging of a greater number of biomolecules in living cells and tissues than is currently attainable.

A novel study from researchers at Karolinska Institutet shows that by using so called random sequence labels, working as barcodes for tracing genetically altered cells, during CRISPR / Cas9 gene knock - outs it is possible to generate more comprehensive data from a smaller number of cells.

Immunofluorescent labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by fluorescently labeled, secondary anti-Ig antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.

Complete phenotyping of the mouse immune system by polychromatic and mass cytometry (CYTOF), thanks to a set of standardised protocols enabling isolation of viable cells from lymphoid and non-lymphoid organs (lung, skin, intestine,...) for labelling using complex ranges of antibodies whose compatibility allows the simultaneous registration of 50 quantitative parameters at least (size, structure, specific antibodies and cell viability).

As an appropriate ECM substrate for cancer cell migration, we use nonpepsinized rat tail collagen I polymerized at room - temperature and fluorescently labeled to facilitate visualization using standard confocal microscopes.

At times, those labels can be incompatible with live cells, or unavailable to researchers.

Immunocytochemistry / Immunofluorescence analysis of human cardiac stem cells labeling Ki67 with ab16667 at 1/250 dilution.

Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.

Immunofluorescent analysis of Hela cells, using SMN2 antibody 11708 -1-AP at 1:25 dilution and Rhodamine - labeled goat anti-rabbit IgG (red).

Importantly, akin to HESC - RPE in vivo, cells at the outside edge of the iPS - RPE cell bolus could phagocytose photoreceptor outer segments from the RCS rat, as indicated by the presence of rhodopsin - positive photoreceptor material within the cellular membrane of iPS - RPE labelled with HSM (Fig. 5E).

Therefore, at the time point used by Knowlden et al, labeled T cells in LNs might have included those that have re-entered the LNs.

To quantitate the proportion of cells in the LN lymphatic sinuses at a given moment in time, we optimized an in vivo procedure to label lymph - exposed cells based on the established method of antibody pulse - labeling of blood - exposed cells (Cinamon et al., 2008).

However, the truth is that the saponins found in many varieties of agave plants are toxic steroid derivatives, capable of disrupting red blood cells and producing diarrhea and vomiting, to be avoided during pregnancy because they might cause or contribute to miscarriage by stimulating blood flow to the uterus.40 At the very least, agave products should carry a warning label indicating that the product may cause a miscarriage.

Results The researchers were able to estimate the amount of growth that took place in the participants» muscle cells by looking at the amount of labelled phenylalanine that they absorbed.

For example, in the following illustration, the data (excluding the column headers and row labels) begins at cell B3 and ends at cell AE50:

How do I handle requests for such references?Thank you so much for your help!CherylCHERYL M. EARLE3407 Old Dobbin Road, Montgomery, Alabama 36116 - 1903Home Phone: [phone] Cell Phone: [phone] Fax: [phone] E-mail: [email protected] position managing legal discovery and document review with opportunity to assist attorneys with civil litigationBAR ADMISSIONAlabama State Bar, 1999LAW - RELATED EXPERIENCELaw Firm, AlabamaResearch Attorney for Special Projects, Mass Torts Department, November 2001 — February 2008 • Managed Multi-District Litigation (MDL) Document Depository (September 2002 to February 2008) o Reviewed more than 1 million pages of evidentiary documents for litigation purposes and for inclusion in electronic databaseso Coordinated document review assignments with attorneys at local depository and at other sites across the USo Retrieved, reviewed and coded documents in Concordance and Summation legal databaseso Prepared memoranda and spreadsheets providing detailed analysis of discovery materials • Aided attorneys and support staff with processing and preparation of personal injury claims and litigationo Conducted legal research and drafted pleadingso Conducted supplementary online research for additional documents and information pertinent to litigationo Assisted with preparation of correspondence to clients and referring attorneyso Contacted clients for additional information needed in case preparation, litigation, and potential settlementso Prepared and input case intakes and referrals into databaseLaw School, AlabamaStudent Intern, Alabama Disabilities Advocacy Program (ADAP), August 1996 — June 1997 • Participated in law school clinical program under third - year law student practice rule (as authorized by Alabama Supreme Court) o Assisted attorneys and advocates in cases involving mentally ill patients confined to state mental health facilitieso Interviewed clients in person (at state facilities) and over the phoneo Worked with clients, attorneys, and social workers to investigate and resolve issues concerning involuntary confinement and treatmento Aided in legal research on an appellate brief submitted to the U. S. Court of Appeals for the Eleventh Circuit (ruling granted in favor of our client) Faculty Research Assistant for Library Services, Bounds Law Library, March 1996 — June 1997 • Prepared research and teaching materials for law school faculty; worked 20 hours per week while matriculating 10 - 15 hours per semester) o Investigated copyright issues related to procuring and reproducing texts for academic useo Conducted legal research using WESTLAW, LEXIS and the InternetADDITIONAL RELEVANT EXPERIENCEManufacturing Company (MC), Montgomery, AlabamaAdministrative Assistant and Cost Analyst, Materials Purchasing Department, April 1999 — September 2001 • Assisted materials buyers in negotiating and preparing commodities contracts between raw materials suppliers and MC for manufacturing plants in the US and Mexicoo Assisted Legal Department at MC's corporate headquarters with coordination and preparation of documents for litigationo Notified and educated suppliers about MC's freight - on - board policy and its corresponding Uniform Commercial Code (UCC) provisions; result was the reduction of freight claims for both the company and its supplierso Prepared contracts and purchase orders for raw materials and capital projects involving plant maintenanceo Solicited price quotations from current vendors and established Excel spreadsheet format which simplified quote submission process and allowed MC to track and compare usage volumes and costs over timeo Prepared and analyzed cost reports used by materials buyers and production planners in purchasing decisions, including cost reductions, materials consolidation, and selection of vendorso Acted as liaison between vendors and the Purchasing, Transportation and Accounting Departments on issues concerning inbound freight, commercial carriers, and payment terms for commodities, resulting in reductions in freight costs and greater payment discounts for raw materialso Established online databases and printed directories for the Purchasing Department, allowing buyers to have easier and faster access to current vendor informationo Completed Year 2000 (Y2K) compliance project, which involved data collection and communication with MC's past, present, and potential materials suppliers and service providersNot - For - Profit Organization, AlabamaAdministrative Assistant, Combined Federal Campaign, September 1998 — January 1999 • Aided Campaign Director with 1998 Federal Campaigns (CFCs) in City 1 and City 2, which together generated nearly $ 700,000 for more than 1,000 local, national and international charitieso Prepared weekly reports on donations using WordPerfect, Microsoft Word, Excel and dBase IVo Wrote script for Talent Showcase at City 1's 1998 CFC Kickoffo Assisted Director with merger of the City 1 and City 2 CFCs in 1999Regional Bank, AlabamaAdministrative Assistant, Year 2000 (Y2K) Department, March — June 1998 • Worked with Vice President of Corporate Projects on short - term project for the bank's Y2K Departmento Analyzed and processed data on Y2K readiness for all branches of Bank throughout the southeastern USo Organized meetings for personnel of Banko Communicated with vendors of computer hardware, software, and office equipment to request information on Y2K complianceo Prepared compliance files for Federal Reserve auditso Prepared in - house memoranda and reports using Microsoft Word and ExcelRecord / Music Promotion Company, AlabamaRecord Pool Co-Founder; Office Manager, September 1990 — December 1991 • Co-founded record pool to enhance promotion of music in Alabama and the southeastern USo Procured and distributed records from major and independent labels for club, radio and mobile disc jockeyso Coordinated jointly sponsored promotional events with record companies, radio stations and clubso Designed, wrote, and published bi-weekly reports and brochures to inform the music industry of the progress and popularity of music and performers in the region, with specific focus on the Alabama music sceneMajor University, AlabamaGraduate Research Assistant, AUM Department of Marketing, June 1989 — August 1990 • Worked 13 - 20 hours per week as a research assistant to Marketing faculty while carrying a full course load in the MBA programo Analyzed consumer surveys used in academic researcho Assisted Conference Chairperson with coordination for Atlantic Marketing Association (AMA) annual meeting (October 1989) o Co-authored five - year index and classification of AMA Proceedings (published Fall 1991) EDUCATIONLaw School, AlabamaJuris Doctor (JD), 1997 • Scholarshipso Seybourn H. Lynne Scholarship, 1996 - 97o Dexter C. Hobbs Memorial Scholarship, 1995 - 96o E. W. Godbey Memorial Scholarship, 1994 - 95 • Honorso Who's Who Among American Law School Students, 1996 - 94o Arthur Davis Shores Award, 1997 • Activitieso Frederick Douglass Moot Court Team Manager, 1996 - 97 Southern Regional Competition, Second Place National Competition, Eighth Placeo John A. Campbell Moot Court Competition, Spring 1996o Black Law Students Association Delegate, BLSA National Convention, 1997 Co-Chairperson, Public Relations Committee, 1996 - 97 Chairperson, Public Relations Committee, 1995 - 96 BLSA President's Award, 1996 and 1997o American Bar Association, 1996 - 97 Entertainment and Sports Industries Forum Intellectual Property Section Law Student Divisiono LAWS Student Group Leader, 1995 - 96Major University, AlabamaMaster of Business Administration (MBA), 1990Bachelor of Science in Business Administration (B.S.B.A.), 1988 (Major: Marketing — Advertising and Promotion Track) • Honorso Dean's List • Activitieso National Student Advertising Competition Team, 1988 - 90 Seventh District Competition: Third Place, 1990o Marketing Club, 1987 - 90 Vice President — Career Development, 1988 - 89o Public Relations / Advertising (PR / AD) Club, 1988 - 90 Charter Member, 1988 Active in fund - raising and membership driveso Theater Guild, 1988 - 90 Screening Committee, 1989REFERENCESAvailable upon request

Lab Technician II — TRL Plasma Laboratories — January 2013 — Present • Monitor the heat sealing and aseptic sampling of plasma units; place units of plasma into freezer in timely manner in order to ensure quality of product • Label plasma samples and units properly and store according to policy; manage an average of 50 different plasma units on a regular basis • Evaluate refrigerator and freezer temperatures ad inform supervisor if equipment is malfunctioning • Maintain efficient and clean work environment and ensure inventory is stocked properly; answer phones and answer questions or transfer calls to appropriate departmentLab Technician I — ABC Medical Technologies, Inc. — May 2007 — January 2013 • Operated laboratory equipment, such as cell counters and microscopes, to analyze urine, blood, and tissue samples; recorded both normal and abnormal findings; had less than a.1 percent margin of error in sample findings • Used computerized instruments and automated equipment to perform multiple tests at one time; maintained calibration and proper function of equipment on a regular basis • Entered data from tests into physician reports; discussed abnormal results with supervisor and re-ran tests before logging data into patient's medical record