Not exact matches
Tubulin is
labeled gold, highlighting the
cell body and flagellum, while the protein actin is
labeled blue, showing off the organism's distinctive collar.
Photo of a living Brainbow zebrafish, taken by Zachary Tobias (a research technician in Weissman - Unni's lab), showing a brightly
labeled neuron with its
cell body (white) at bottom.
Now, a new approach developed by Dr. Ekaterina Galanzha of the University of Arkansas for Medical Sciences in Little Rock and her colleagues allows for
labeling and tracking of individual circulating cancer
cells throughout the
body, thereby helping researchers elucidate the pathways of single
cells from start to finish.
To better understand what happens during immune reactions in the
body, researchers at Tübingen University have developed a new way of
labeling T -
cells, allowing them to track the T -
cell movement in mice using non-invasive positron emission technology (PET).
The researchers then attempted to see if the
cells, which they
labeled multipotent adult germline stem
cells (maGSC), would differentiate into specific types of
body cells.
He used an assortment of anatomical tracing techniques to
label neurons in their entirety — a
cell body with a long axon extending out in one direction and the branched tendrils of dendrites protruding from the other.
Figure 2: Nerve
cell bodies and fibers
labeled with rabbit anti-PGP + donkey anti-rabbit-Cy3 (711-225-152 green), vascular basement membranes
labeled with collagen IV + donkey anti-mouse-Cy2 (715-225-150 red) and DAPI -
labeled DNA (blue) in jejunum submucosal ganglion.
The
labelling is for acetylated tubulin in red (identifying all axons), and green for the
cell permeable dye calcein, which is only applied on the axonal side of the chambers (top half) and allows the identification of those neuronal
cell bodies (bottom half) that have extended axons to the other side of the microfluidic device.
Images of the
body can be taken of the
labeled peptides as they bind, revealing where stray tumor
cells may be nesting.
As the intent is to non-toxically
label live
cells for whole
body and deep tissue imaging, multimerization should not be an issue.