Sentences with phrase «labeled cells into»

Not exact matches

Cyclic adenosine monophosphate (cAMP)- dependent protein kinase, labeled with fluorescein and rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in culture or in intact cell clusters.

When the piglets were 2 days old, the scientists injected fluorescently labeled cell trackers into a key brain area, the subventricular zone (SVZ).

«When we transplanted our labeled blood stem cells from the bone marrow into other mice, only a few stem cells were active in the recipients, and many stem cells were lost,» Rodewald explains.

Greber and his team infected human cells in culture with the chemically labeled viruses, and observed the behavior of the viral DNA during entry into cells.

Now you know that if you take a mouse embryonic stem cell that is, say, labeled so all of the developed cells will be blue, and you inject it into a mouse blastocyst, you'll get a chimera.

To test this, the team infected monocytes with a form of Toxo, labeled red, that can't reproduce, then introduced those cells into mice.

This method, known as targeted radionuclide therapy, or TRT, involves the use of molecules labeled with radioactive atoms that are injected into patients and localized in cancer cells.

The team went on to show that supplementing heavy stable isotope - labeled forms of these precursors led to incorporation of heavy amino acids into proteins produced in enzyme expressing cells.

Rolls and her colleagues compared the ability of fluorescently labeled stem cells from sleepy and from rested mice to migrate properly from the recipients» blood into the bone marrow.

«We showed that the presence of the maternal gut microbiota during late pregnancy blocked the passage of labeled antibodies from the circulation into the brain parenchyma of the growing fetus,» says first author Dr. Viorica Braniste at the Department of Microbiology, Tumor and Cell Biology at Karolinska Institutet.

The researchers then attempted to see if the cells, which they labeled multipotent adult germline stem cells (maGSC), would differentiate into specific types of body cells.

Before ending the experiment, the mice were injected with a compound that's selectively incorporated into dividing cells, and hence labels adult - born neurons.

They removed spermatogonia labeled with a fluorescent marker from a mature male trout and injected the cells into the abdomens of newly hatched male and female trout fry.

The researchers injected a compound normally used to label tumor cell division into patients who had agreed to have their brains examined after death.

For Matrigel invasion assay, CSCs were labeled with Cell tracker green (Invitrogen) and 50,000 cells were seeded into Matrigel - coated Transwell insert (Corning) supplemented with DMEM with 10 % serum.

CSCs were labeled with Cell tracker green and 50,000 cells were seeded into Transwell inserts supplemented with DMEM with 10 % serum.

Two days later, the cells assembled into a 5 - millimetre - long, three - dimensional tissue that the researchers labelled a liver bud — an early stage of liver development.

During fear conditioning, researchers used a virus to deliver a gene into the dentate gyrus, which labeled active engram cells.

They found that injecting into the carotid artery breast cancer cells that express markers allowing them to enter the brain — cells labelled with bioluminescent and fluorescent markers to enable tracking by imaging technologies — resulted in the formation of many metastatic tumors throughout the brain, mimicking what is seen in advanced breast cancer patients.

Initial imaging analysis of fluorescently labelled human iPSC - derived hepatic endoderm cells, umbilical cord - derived endothelial cells (HUVECs), and mesenchymal stem cells (MSCs) co-cultured in a solidified matrix gel to promote 3D growth found that the different cells collectively and automatically «condensed» into a multicellular central unit.

They hypothesized that, if they labeled the parent cell population using the library, propagated it, separated it into roughly identical replicate groups, and treated these groups with the same drug regimen, they would find one of two outcomes (see schematic depiction below):

The videos confirmed the unorthodox new mode of division Obernier's labeling experiments had implied, but also revealed the cells» surprising dynamism: a long tail that touches and probes nearby blood vessels, short arms that poke and prod other stem cells, and a tongue - like antenna that pokes into the nearby ventricular space, as if to «taste» the cerebrospinal fluid there.

Lpar2 - / - CD4 + T cells were labeled with 5 μM CMTMR (Thermo Fisher Scientific), mixed with an equivalent number of WT CD4 + T cells, and injected into recipient mice (5 x 106 cells / mouse); 15 — 25 hr later, the mice were anesthetized using isoflurane admixed with O2.

The biotin - labeled cells were injected intravenously into recipient mice (2 x 107 cells / mouse), and the LNs and spleen were harvested 1.5 hr later.

Radiocarbon analyses of 14C in lipid biomarkers associated with marine plankton [14], and 13C - labeled bicarbonate tracer studies [15] suggest that marine Crenarchaeota are capable of light - independent autotrophic carbon assimilation into membrane lipid biomass, an hypothesis further strengthened by recent single cell phylogenetic identification and autoradiographic verification of carbon dioxide incorporation [16].

Brain cells pick up the labeled leucine over the course of hours and it's incorporated into new copies of amyloid - beta and other proteins they make.

How do I handle requests for such references?Thank you so much for your help!CherylCHERYL M. EARLE3407 Old Dobbin Road, Montgomery, Alabama 36116 - 1903Home Phone: [phone] Cell Phone: [phone] Fax: [phone] E-mail: [email protected] position managing legal discovery and document review with opportunity to assist attorneys with civil litigationBAR ADMISSIONAlabama State Bar, 1999LAW - RELATED EXPERIENCELaw Firm, AlabamaResearch Attorney for Special Projects, Mass Torts Department, November 2001 — February 2008 • Managed Multi-District Litigation (MDL) Document Depository (September 2002 to February 2008) o Reviewed more than 1 million pages of evidentiary documents for litigation purposes and for inclusion in electronic databaseso Coordinated document review assignments with attorneys at local depository and at other sites across the USo Retrieved, reviewed and coded documents in Concordance and Summation legal databaseso Prepared memoranda and spreadsheets providing detailed analysis of discovery materials • Aided attorneys and support staff with processing and preparation of personal injury claims and litigationo Conducted legal research and drafted pleadingso Conducted supplementary online research for additional documents and information pertinent to litigationo Assisted with preparation of correspondence to clients and referring attorneyso Contacted clients for additional information needed in case preparation, litigation, and potential settlementso Prepared and input case intakes and referrals into databaseLaw School, AlabamaStudent Intern, Alabama Disabilities Advocacy Program (ADAP), August 1996 — June 1997 • Participated in law school clinical program under third - year law student practice rule (as authorized by Alabama Supreme Court) o Assisted attorneys and advocates in cases involving mentally ill patients confined to state mental health facilitieso Interviewed clients in person (at state facilities) and over the phoneo Worked with clients, attorneys, and social workers to investigate and resolve issues concerning involuntary confinement and treatmento Aided in legal research on an appellate brief submitted to the U. S. Court of Appeals for the Eleventh Circuit (ruling granted in favor of our client) Faculty Research Assistant for Library Services, Bounds Law Library, March 1996 — June 1997 • Prepared research and teaching materials for law school faculty; worked 20 hours per week while matriculating 10 - 15 hours per semester) o Investigated copyright issues related to procuring and reproducing texts for academic useo Conducted legal research using WESTLAW, LEXIS and the InternetADDITIONAL RELEVANT EXPERIENCEManufacturing Company (MC), Montgomery, AlabamaAdministrative Assistant and Cost Analyst, Materials Purchasing Department, April 1999 — September 2001 • Assisted materials buyers in negotiating and preparing commodities contracts between raw materials suppliers and MC for manufacturing plants in the US and Mexicoo Assisted Legal Department at MC's corporate headquarters with coordination and preparation of documents for litigationo Notified and educated suppliers about MC's freight - on - board policy and its corresponding Uniform Commercial Code (UCC) provisions; result was the reduction of freight claims for both the company and its supplierso Prepared contracts and purchase orders for raw materials and capital projects involving plant maintenanceo Solicited price quotations from current vendors and established Excel spreadsheet format which simplified quote submission process and allowed MC to track and compare usage volumes and costs over timeo Prepared and analyzed cost reports used by materials buyers and production planners in purchasing decisions, including cost reductions, materials consolidation, and selection of vendorso Acted as liaison between vendors and the Purchasing, Transportation and Accounting Departments on issues concerning inbound freight, commercial carriers, and payment terms for commodities, resulting in reductions in freight costs and greater payment discounts for raw materialso Established online databases and printed directories for the Purchasing Department, allowing buyers to have easier and faster access to current vendor informationo Completed Year 2000 (Y2K) compliance project, which involved data collection and communication with MC's past, present, and potential materials suppliers and service providersNot - For - Profit Organization, AlabamaAdministrative Assistant, Combined Federal Campaign, September 1998 — January 1999 • Aided Campaign Director with 1998 Federal Campaigns (CFCs) in City 1 and City 2, which together generated nearly $ 700,000 for more than 1,000 local, national and international charitieso Prepared weekly reports on donations using WordPerfect, Microsoft Word, Excel and dBase IVo Wrote script for Talent Showcase at City 1's 1998 CFC Kickoffo Assisted Director with merger of the City 1 and City 2 CFCs in 1999Regional Bank, AlabamaAdministrative Assistant, Year 2000 (Y2K) Department, March — June 1998 • Worked with Vice President of Corporate Projects on short - term project for the bank's Y2K Departmento Analyzed and processed data on Y2K readiness for all branches of Bank throughout the southeastern USo Organized meetings for personnel of Banko Communicated with vendors of computer hardware, software, and office equipment to request information on Y2K complianceo Prepared compliance files for Federal Reserve auditso Prepared in - house memoranda and reports using Microsoft Word and ExcelRecord / Music Promotion Company, AlabamaRecord Pool Co-Founder; Office Manager, September 1990 — December 1991 • Co-founded record pool to enhance promotion of music in Alabama and the southeastern USo Procured and distributed records from major and independent labels for club, radio and mobile disc jockeyso Coordinated jointly sponsored promotional events with record companies, radio stations and clubso Designed, wrote, and published bi-weekly reports and brochures to inform the music industry of the progress and popularity of music and performers in the region, with specific focus on the Alabama music sceneMajor University, AlabamaGraduate Research Assistant, AUM Department of Marketing, June 1989 — August 1990 • Worked 13 - 20 hours per week as a research assistant to Marketing faculty while carrying a full course load in the MBA programo Analyzed consumer surveys used in academic researcho Assisted Conference Chairperson with coordination for Atlantic Marketing Association (AMA) annual meeting (October 1989) o Co-authored five - year index and classification of AMA Proceedings (published Fall 1991) EDUCATIONLaw School, AlabamaJuris Doctor (JD), 1997 • Scholarshipso Seybourn H. Lynne Scholarship, 1996 - 97o Dexter C. Hobbs Memorial Scholarship, 1995 - 96o E. W. Godbey Memorial Scholarship, 1994 - 95 • Honorso Who's Who Among American Law School Students, 1996 - 94o Arthur Davis Shores Award, 1997 • Activitieso Frederick Douglass Moot Court Team Manager, 1996 - 97 Southern Regional Competition, Second Place National Competition, Eighth Placeo John A. Campbell Moot Court Competition, Spring 1996o Black Law Students Association Delegate, BLSA National Convention, 1997 Co-Chairperson, Public Relations Committee, 1996 - 97 Chairperson, Public Relations Committee, 1995 - 96 BLSA President's Award, 1996 and 1997o American Bar Association, 1996 - 97 Entertainment and Sports Industries Forum Intellectual Property Section Law Student Divisiono LAWS Student Group Leader, 1995 - 96Major University, AlabamaMaster of Business Administration (MBA), 1990Bachelor of Science in Business Administration (B.S.B.A.), 1988 (Major: Marketing — Advertising and Promotion Track) • Honorso Dean's List • Activitieso National Student Advertising Competition Team, 1988 - 90 Seventh District Competition: Third Place, 1990o Marketing Club, 1987 - 90 Vice President — Career Development, 1988 - 89o Public Relations / Advertising (PR / AD) Club, 1988 - 90 Charter Member, 1988 Active in fund - raising and membership driveso Theater Guild, 1988 - 90 Screening Committee, 1989REFERENCESAvailable upon request

Lab Technician II — TRL Plasma Laboratories — January 2013 — Present • Monitor the heat sealing and aseptic sampling of plasma units; place units of plasma into freezer in timely manner in order to ensure quality of product • Label plasma samples and units properly and store according to policy; manage an average of 50 different plasma units on a regular basis • Evaluate refrigerator and freezer temperatures ad inform supervisor if equipment is malfunctioning • Maintain efficient and clean work environment and ensure inventory is stocked properly; answer phones and answer questions or transfer calls to appropriate departmentLab Technician I — ABC Medical Technologies, Inc. — May 2007 — January 2013 • Operated laboratory equipment, such as cell counters and microscopes, to analyze urine, blood, and tissue samples; recorded both normal and abnormal findings; had less than a.1 percent margin of error in sample findings • Used computerized instruments and automated equipment to perform multiple tests at one time; maintained calibration and proper function of equipment on a regular basis • Entered data from tests into physician reports; discussed abnormal results with supervisor and re-ran tests before logging data into patient's medical record