Scientists at the Max Planck Institute of Biochemistry in Martinsried, Germany, now discovered a new family of helper proteins that recognize
labeled cellular protein waste and guide them efficiently to the lysosome for destruction and subsequent recycling into their reusable compounds.
Not exact matches
This latter capability is extremely valuable to biomedical research, which relies heavily on fluorescent probes to
label and track
proteins, nucleic acids, and other
cellular components.
Newly synthesized adenoviral DNA
labeled by click chemistry is depicted in red, a viral DNA - binding
protein in green, and the
cellular DNA in blue.
When the researchers added radio -
labelled arsenate to the solution to track its distribution, they found that arsenic was present in the
cellular fractions containing the bacterium's
proteins, lipids and metabolites such as ATP and glucose, as well as in the nucleic acids that made up its DNA and RNA.
Oligonucleotide -
labeled antibodies allow integration of
cellular protein and transcriptome measurements at a single - cell level, with the number of simultaneously assayed
protein markers far surpassing what can be measured by cytometry - based approaches.
For example we can visualize fluorescently
labeled proteins and compare the quantity and localization relative to other
labeled cellular components.