Sentences with phrase «labeled secondary antibodies used»

Not exact matches

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

Immunoreactions were developed using a fluorescently labeled secondary antibody, and samples visualized in a Leica Biosystems epifluorescent microscope or using a secondary antibody linked to horseradish peroxidase and developed with a 3,3 ′ - diaminobenzidine kit.

Immunofluorescent labeling was performed using anti-Olig2 (1 ∶ 4000, Chemicon) and anti-GFAP (1 ∶ 400, Cell Signaling) followed by fluorescently labeled, secondary anti-Ig antibodies (Alexa 488 and 568 conjugates, Invitrogen) at a 1 ∶ 2000 dilution.

Antibody binding was visualized by chemiluminescence detection in a CCD - camera system using a peroxidase (HRP) labeled secondary aAntibody binding was visualized by chemiluminescence detection in a CCD - camera system using a peroxidase (HRP) labeled secondary antibodyantibody.

Secondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from InvitrogSecondary labeling was carried out using isotype matched Alexa - fluor labeled secondary antibodies (AF488, 568 and 594, all from Invitrogsecondary antibodies (AF488, 568 and 594, all from Invitrogen Corp).

Appropriately coupled secondary antibodies (Molecular Probes) were used for single and double labeling.