Sentences with phrase «labeling cells for»

However, such multimerization capabilities should not interfere with the intended goal of non-toxically labeling cells for deep tissue and intravital imaging as the Amrose variants 1 — 3 were developed in vertebrate cells.

Targeting the labeled cells for analysis, they revealed that their organoids contained a population of sensory cells that have the same functional signature as cells that detect gravity and motion in the human inner ear.

Not exact matches

In a segment he labeled «The Old Man and the Cell,» The Daily Show «s Jon Stewart mercilessly mocked Congressman Charlie Rangel last night for a pretend phone call he made during a debate earlier this week.

Her PhD is focused on developing a novel label - free imaging approach for assessing human stem cells and skeletal regeneration non-destructively and non-invasively.

HeLa cells, with proteins labeled in blue and DNA in red, are responsible for over 20 percent of the cell line contaminations.

Labeling biomolecules with light - emitting nanoparticles is a powerful technique for observing cell movement and signaling under realistic, in vivo conditions.

By developing a new technique for labeling the gene segments of influenza viruses, researchers now know more about how influenza viruses enter the cell and establish cell co-infections — a major contributing factor to potential pandemic development.

The study, which was led by postdoc Gregor Pilz and PhD student Sara Bottes, used in vivo 2 - photon imaging and genetic labeling of neural stem cells in order to observe stem cell divisions as they happened, and to follow the maturation of new nerve cells for up to two months.

The new AAV vectors can also deliver genes that code for colorful fluorescent proteins; such proteins are useful in identifying and labeling cells.

It has long been known that cells tag proteins for degradation by labelling them with ubiquitin, a signal described as «the molecular kiss of death.»

The technique, named cell type specific labeling using amino acid precursors (CTAP), exploits the inability of vertebrate cells to synthesize essential amino acids normally required for growth and homeostasis.

Using their own recently - developed genetic labeling techniques for tracking these cells in early development in mice, Taniguchi and his team observed that, like most neurons, the cells remodeled their axonal organization through development.

Using quantitative mass spectrometry to search for proteins that contained these stable isotope labels, researchers were able to determine the cell of origin of both intracellular and secreted proteins identified in multicellular culture.

«This is extremely important for labelling which cells are being monitored.»

They are examining every aspect of cell function and cell structure, looking for clusters of phenotypes that could label a patient's cancer so precisely that it could be linked to therapies proven effective against just that type.

«This technology allows for the labeling of just one circulating pathological cell among billions of other normal blood cells by ultrafast changing color of photosensitive proteins inside the cell in response to laser light,» explains Dr. Galanzha.

The proteins that bacteria secrete are labeled with unique amino acid sequences, which tell the cells that the proteins are destined for secretion.

Now, a new approach developed by Dr. Ekaterina Galanzha of the University of Arkansas for Medical Sciences in Little Rock and her colleagues allows for labeling and tracking of individual circulating cancer cells throughout the body, thereby helping researchers elucidate the pathways of single cells from start to finish.

Proteins were labeled with isotopes — a technique similar to carbon dating for archaeological finds — and followed as cells underwent heat shock and recovery.

They sent soil samples for DNA testing, looking for matches with particular genes known to be found in microbes and fungi; they tried to stimulate microbial growth on a wide variety of substances and then count the cells produced; and they used highly sensitive radiorespiration activity assays, which involve feeding the soil microorganisms a food source which has been labelled with radioactive carbon, which can then be used to detect if the microorganisms are active.

For Matrigel invasion assay, CSCs were labeled with Cell tracker green (Invitrogen) and 50,000 cells were seeded into Matrigel - coated Transwell insert (Corning) supplemented with DMEM with 10 % serum.

For quantification of FluoroGold labeling, cells were scored in a binary fashion (yes or no) for presence of FluoroGold and / or GFor quantification of FluoroGold labeling, cells were scored in a binary fashion (yes or no) for presence of FluoroGold and / or Gfor presence of FluoroGold and / or GFP.

The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.

Our physiological analysis established that functional 5 - HT3 receptors are present only in these GFP - labeled ganglion cells and that 5 - HT3 is required for ganglion cell responses to 5 - HT.

Specifically, cell cultures from bone tissue were incubated in 10 % DMEM containing 10 ng / ml TNF - α and 10 μg / ml fluorescent probe of acetylated LDL, DiI - Ac - LDL (Biochemical Technologies, Cambridge, MA), for 4 h. Cells were harvested in the manner described above with the exception that during this labeling period, the rat anti-E-selectin mAb (10E9.6) conjugated to FITC replaced PE - conjugated 10E9.6.

Cells above threshold were considered positive for label.

Cells were washed twice with label - free medium, and the slides were fixed in 4 % paraformaldehyde for 10 min.

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldeCells were washed with label - free medium and fixed in 4 % paraformaldehyde.

Endothelial cells that had undergone two sessions of FACS - based selection were evaluated for their ability to metabolize fluorescence - labeled acetylated LDL (DiI - Ac - LDL).

Cells treated with hamster anti-αβTCR or hamster murine γδTCR were washed three times in HBSS and incubated in PE - labeled Armenian hamster antihamster IgG (PharMingen) secondary antibody for 20 min at 4 °C, washed three additional times in PBS, fixed in 1 % paraformaldehyde, and assessed for fluorescence in a FACScan flow cytometer (Becton Dickinson).

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

«If you went back a few years and opened a textbook you'd see the classic hippocampal circuit that's been diagrammed for a hundred years, and you'd look at the subiculum and you'd see it labeled as one region, with one type of cell, doing one thing,» Cembrowski explains.

14255: STAT3 Signaling During Chemo - Radiation for Squamous Cell Carcinoma of the Head and Neck 14340: A Phase II, Randomized, Open - Label, Multi-Center, Global Study of MEDI4736 Monotherapy, Tremelimumab Monotherapy, and MEDI4736 in Combination with Tremelimumab in Patients with Recurrent or Metastatic Squamous Cell Carcinoma of the Head and Neck (SCCHN) 14258: Randomized Phase II and Phase III Studies of Individualized Treatment for Nasopharyngeal Carcinoma Based on Biomarker Epstein Barr Virus (EBV) Deoxyribonucleic Acid (DNA)

Bipolar (H), amacrine (I), and retinal ganglion (J) cells double labeled for YFP and Calretinin appear reddish - yellow.

For example, labeling of these cells with stem - like cell associated markers (p63, nestin, and telomerase) have heavily implicated these cells as stem cells [13].

For leadership and pioneering contributions in the field of biophotonics, comprising the diverse use of label - free native fluorescence, Raman spectroscopy, and optical imaging for cancer detection in tissues and celFor leadership and pioneering contributions in the field of biophotonics, comprising the diverse use of label - free native fluorescence, Raman spectroscopy, and optical imaging for cancer detection in tissues and celfor cancer detection in tissues and cells.

(D) Cryostat section (12 µm) shows cells labeled for the retinal ganglion cell (RGC) marker, hermes, by in situ hybridization.

Host (YFP) cells appear red when stained for cell class specific markers, while double - labeled, donor - derived cells (arrows, arrowheads, and carrots) appear yellowish - red.

Synthesis of 7 - Aminocoumarin by Buchwald — Hartwig Cross Coupling for Specific Protein Labeling in Living Cells.

In the current study, lead authors David V. Hansen, PhD, a postdoctoral fellow, and Jan H. Lui, a graduate student in the Kriegstein lab, examined the OSVZ, using new labeling and tracking techniques to follow individual cells and their progeny over time in cultured tissue slices from fetal cortex tissue that had been donated for research.

Control and gesicle - treated hiPS cells were assessed for AcGFP1 expression (green), labeled with an antibody specific to pluripotency marker Oct ‑ 4, visualized with a fluorescent - labeled secondary antibody (red), and nuclear - labeled with DAPI (blue).

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.

In the late 1960s, Rowley and his graduate student Donald Mosier were the first to describe the function of a previously unrecognized cell type, a component of the adaptive immune system they labeled the «A cell,» for its «adherent, accessory or antigen - presenting capacity.»

With the recent development of transgenic mouse models, which allow labelling and manipulation of specific cell types, more and more emphasis has been placed on the tissue scale: How does the collective behavior of many cells gives rise to the architecture that is characteristic for given kind of tissue?

PromoKine offers a wide range of well proven products for cell biology research such as kits and reagents for cell analysis, apoptosis research, cell transfection, fluorescent labeling, gene cloning & expression, mycoplasma detection and elimination as well as numerous antibodies, ELISAs, cytokines and growth factors.

High contrast images facilitate robust automatic tracking and behavioural analysis of hundreds of individual cells within heterogeneous cell populations is now possible without the need for intrusive dyes or labels, bringing unique morphological, temporal, and dynamic phenotypic data to your research.

For double labeling, the cells were incubated with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally with avidin - rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).

The Bulte Lab has developed methods to label cells magnetically using tiny superparamagnetic iron oxide nanoparticles in order to make them visible by magnetic resonance imaging; this technology has now been introduced in the clinic for several cell therapy applications.

Researchers at Columbia University have made a significant step toward breaking the so - called «color barrier» of light microscopy for biological systems, allowing for much more comprehensive, system - wide labeling and imaging of a greater number of biomolecules in living cells and tissues than is currently attainable.

The labelling is for acetylated tubulin in red (identifying all axons), and green for the cell permeable dye calcein, which is only applied on the axonal side of the chambers (top half) and allows the identification of those neuronal cell bodies (bottom half) that have extended axons to the other side of the microfluidic device.