Sentences with phrase «labelled antibodies which»

Wellington and her colleagues detected the dormant cells with fluorescent - labelled antibodies which cling specifically to S. typhimurium.

Not exact matches

They also utilized immunogold labeling — in which gold particles are attached to antibodies that bind to particular proteins in order to make them visible in electron microscopy — to show that filaments within the feathers were keratin.

Labeled antibodies, which bind to specific cell types, allowed the researchers to determine which types of neuron were being created at which times.

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

(E) R5 antibody labels Müller glia (Mü; red), which extend processes the breadth of the flank retina.

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

At 13 months of age, the intracellular MOAB - 2 immunolabel was hardly detectable in pyramidal neurons adjacent to amyloid plaques, the latter which were strongly labeled by this antibody and not by pab27576 (Additional file3: Figure S2 a-b).

Secondary antibody (Affinity Purified Antibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour aantibody (Affinity Purified Antibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour aAntibody Peroxidase Labeled Goat Anti-Human IgG (γ), KPL, Gaithersburg, Maryland, United States: Cat # 074 - 1002) at a 1 ∶ 4,000 dilution, was added and incubated for 1 hour at 22 °C, after which substrate (ABTS Peroxidase Substrate System (2 - Component), KPL: Cat # 50-62-01) was added and incubated for an additional hour at 22 °C.

(E) Dilp5 in the IPCs was detected by anti-Dilp5 antibody in wild - type and CCHa2 - R mutants, in which the IPCs were labeled by dilp2 - GAL4 driving UAS - mCD8:: GFP.

However, the morphology of the host inner retinal cells was well - preserved in the area of donor cell migration, as evident from the PKCα antibody staining, which labeled normal - appearing rod bipolar cell dendrites (upward arrows in Figure 6B).

The researchers used an antibody that labels the proteins made by two closely linked homeotic genes, Ubx and abdA, to show in which segments of the embryo the genes are turned on.

(C) Dilp2 in the IPCs was detected by Dilp2 antibody in wild - type and CCHa2 - R mutants in which the IPCs were labeled by dilp2 - GAL4 - driven UAS - mCD8:: GFP.

A few years later, Karl Deisseroth's lab developed CLARITY, which is a hydrogel - based method that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).