Sentences with phrase «labelled using fluorescent»

T - tubules of isolated cardiomyocytes from rodent models of cardiac diseases are labelled using fluorescent dextran that diffuses from extracellular space to TATS lumen.

Not exact matches

It will ship the first sequencers that use fluorescent labeling this year, and plans to add the methylation mapping capability next year.

The system uses an enzyme called DNA polymerase to read a strand of DNA and build a complementary strand out of nucleotides labeled with fluorescent molecules.

Using fluorescent labels, they were able to track the fates of marked and unmarked chromosomes under the microscope, from egg cells and sperm to the dividing cells of embryos after fertilization.

Developmental biologist Francesca Peri of the European Molecular Biology Laboratory in Heidelberg, Germany, and colleagues created genetically engineered versions of the animals that produced microglia labelled with green fluorescent protein, a glowing compound frequently used in laboratory research.

This novel fluorescent label - free approach uses the lateral shifts in the position of the microbead substrate in pillar arrays, for quantifying the biomolecules, based on the change in surface forces and size, without the need of any external equipment.

Typically, detection of biomolecules such as proteins are performed using colorimetric assays or fluorescent labelling with a secondary antibody for detection, and requires complex optical detection equipment such as fluorescent microscopy or spectrophotometry.

Using new technologies for labeling cellular machinery with light - activated fluorescent markers, they could «turn on» just one molecule at a time.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

Biologists at the University of Cambridge used fluorescent labelling to stain cell membranes in skate embryos, and tracked them through the dynamic development process.

To generate an image, all of the fluorescent labels in a protein are switched on, then a wave of light is used to deactivate most of them.

The most common probes, which use fluorescent markers or radioactive labels to pinpoint the virus's location in a tissue sample, sometimes have difficulty distinguishing the target — HIV RNA and DNA — from surrounding cellular components.

A thalamocortical, or TC neuron labeled with fluorescent dye, as used in Dr. Augustinaite's study.

Approach: Wu's group used Brainbow, a transgenic technique originally devised to map individual neurons in the brain, to label cells stochastically using gene cassettes that code for four uniquely expressed fluorescent proteins.

By labeling one of the shell proteins with Yellow Fluorescent Protein (YFP), we were able to use confocal fluorescence microscopy to observe punctuate loci forming inside chloroplasts.

One common complaint about using four - color Confetti to label single cells (in an animal) is that the cells express green fluorescent protein less often, or sometimes not at all, compared with cells that express each of the other three colors.

Most colocalization methods are based on pixel overlap between the previously denoised signal that is emitted from two (or more) different fluorescent labels, and use a global image correlation such as Pearson's of Manders» coefficients.

NUS Engineering researchers have developed a low - cost microfluidic chip that can quickly and accurately detect and quantify nano - bioparticles using only a standard laboratory microscope without any fluorescent labels.

Many popular methods, such as antibody labeling, are used to add physical fluorescent labels to specific cellular constituents.

Using fluorescent labels that switch on and off, researchers activate and deactivate fluorescent molecules in specific regions at specific times.

Using fluorescent labels with unlabeled images to train a deep neural network to bring out image detail.

«It tells you about the ecosystem of the tumour», Simona says, «using fluorescent markers, we can label many specific cell types and structures within the brain in the vicinity of the tumour, and look not just at the tumour cells but how they interact with their surroundings.

He used a fluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likfluorescent marker called GFP (Green Fluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - likFluorescent Protein) to label the suspected prion - determining domains of these proteins and looked to see if they aggregated in cells - a sign that they may be misfolding and an indication of prion - like activity.

However, we recently developed AAV (adeno - associated virus)- based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals such as rats (17).

Because the embryo is transparent it is possible to use fluorescent proteins to label specific cells and their components, and then use this to watch the fate of the cells in the presence or absence of different genes.

We develop methods to label RNA in fixed and living cells using fluorescent probes and microscopy techniques and image analysis algorithms to visualize and quantify many mRNAs simultaneously.

Researchers at SciLifeLab / Uppsala University headed by Johan Elf have used a combination of microfluidics and image analysis of E. coli with fluorescent labels on the replication machinery to study how the replication and division cycles are coupled in individual cells.

Such an optical contrast exceeded the optical contrast of the fluorescent labels (that were targeted to C4 - 2B and HS - 5 cells using the same prostate cancer - specific PSMA antibody, see Figure 7B, D) by 31 times.

As well as encouraging students and staff to follow good housekeeping measures - such as turning off lights in rooms that aren't in use, making use of natural sunlight, and clearly labeling light switches - converting lighting to LED is a great way for schools to cut down their energy consumption, as LED bulbs use up to 90 % less energy than incandescent lighting (including halogen), and 60 % less than fluorescent lighting.

«I could have a breakfast meeting at PennVet with the leading dog genetics and neurosurgery experts, then lunch at Wistar where they taught me how to use fluorescent labeling to mark the DNA and then walk to the medical college for a meeting about the technical difficulties making holders for different sized dog heads (they are not similar like human heads.)»