Sentences with phrase «laboratory cell lines»

The researchers replicated these three PKC alpha gene variants in laboratory cell lines.

The study involved laboratory cell lines of human leukemia and mouse models of the disease.

Analysis of viral genomic and human mitochondrial sequences revealed that all previously characterized XMRV strains are identical and that the archival RNA had been contaminated by an XMRV - infected laboratory cell line.

Not exact matches

To create the effect of tobacco smoke on cells, Vaz, Baylin and their colleagues began their studies with human bronchial cells, which line the airways of the lungs, and grew them in a laboratory.

Although the researchers emphasized that laboratory results involving cell lines and mice do not necessarily translate to human treatment, they say their findings show that new mTOR inhibitors combined with chemotherapy could become a new treatment strategy for T - ALL.

Focusing on energy - transfer, they measured the impact on cell lines in a laboratory of 7 substances, the clinically - approved drug stiripentol, 3 natural products — caffeic acid phenyl ester (CAPE), silibinin and ascorbic acid — and experimental pharmaceuticals, such as actinonin, FK866 and 2 - DG.

According to Izpisúa Belmonte, who is also a professor at the gene expression laboratories of the Salk Institute for Biological Studies in San Diego, California, CMRB aims to become «a research centre of excellence in south Europe in the line of world - recognized institutions such as the Salk or the Whitehead institutes, where both pre - and postdoctoral researchers receive multidisciplinary training of the highest quality» in stem cell biology and cell regeneration.

As those cells proliferated in laboratory dishes, the bits of human DNA were also copied, creating cell lines, each of which had a different fragment.

The scientists then tested three new mTOR inhibitors currently under development (pp242, AZD8055 and INK128) in combination with the chemotherapies AraC, Etoposide and Cisplatin to see how they affected laboratory lines of leukemia cells and mouse models of the disease.

One of Haugen's researchers discovered that many thyroid cell lines their laboratory stocked and studied were either misidentified or contaminated by other cancer cells.

Today, MD Anderson urges all its scientists to fingerprint new cell lines as soon as they arrive in the lab and before publishing any data, and to complete an annual revalidation of all lines in their laboratories.

Cell lines are the workhorses of biology, routinely stocked and studied in every laboratory to understand cellular pathways, receptors, targets, hormones, and all aspects of normal and malignant physiology.

The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.

A study combining tumor cells from patients with breast cancer with a laboratory model of blood vessel lining provides the most compelling evidence so far that a specific trio of cells is required for the spread of breast cancer.

Researchers remove these mechanically and, with some luck, can nurture them into an embryonic stem cell line that lives in perpetuity in the laboratory.

Apart from a few studies in mouse models and in cell lines, there is no laboratory evidence that synthetic phosphoethanolamine works as a cancer drug.

In studies of laboratory - grown human tumor cell lines, the drug disrupted tumor cell division and prevented growth of advanced cancer cells.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

The article, «The Genomic and Transcriptomic Landscape of a HeLa Cell Line,» by Landry et al., was authored by scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany, and was published in an early online version March 11, 2013.

In May 2001, the U.S. Justice Department charged two Japanese - born scientists with conspiring to «benefit a foreign government» by stealing trade secrets in the form of cell lines and DNA samples from a laboratory at the Cleveland Clinic Foundation in Ohio, where one had worked from 1997 to 1999 (Science, 18 May 2001, p. 1274).

Salk scientists and colleagues have proposed new molecular criteria for judging just how close any line of laboratory - generated stem cells comes to mimicking embryonic cells seen in the very earliest stages of human development, known as naïve stem cells.

The current study was based on previous studies in the laboratory of Nita Ahuja, M.D., director of the Sarcoma and Peritoneal Surface Malignancy Program and professor of surgery at the Johns Hopkins University School of Medicine, which showed that guadecitabine limited the growth of colorectal cancer cell lines when combined with irinotecan, says Azad.

In the laboratory, knocking out PRMT5, or another core component of the splicing machinery in human lymphoma cells lines, also reduced cell viability.

Dr. Bauman and her colleagues treated human head and neck cancer cells in the laboratory with varying doses of sulforaphane and a control, and compared them to normal, healthy cells that line the throat and mouth.

Researchers believe it is the largest epigenetic study yet for any single cancer type and, importantly, the first to use a large cohort of primary patient tumor tissues instead of cell lines grown in the laboratory.

Our investigations on human cell lines in the laboratory have shown that synthetic cannabinoids, in the high concentrations found in cells in the oral cavity or in the lungs, for example, are likely to trigger damage to the DNA that may have significant consequences for the consumers of such substances.

Working with mouse prostate cancer cell lines in the laboratory, the investigators found that cells containing overexpressed TOP2A and EZH2 genes were highly sensitive to attack with a combination of two drugs.

Its first target was the regulatory network involved in controlling the differentiation of THP - 1 cells, a line of human leukemia cells used in laboratory experiments.

On 11 March, the European Molecular Biology Laboratory (EMBL) issued a press release proudly announcing that a research team there had deciphered much of the genetic sequence of one of the most widely used cell lines in cancer studies and had made the information available publicly.

A549 (human lung adenocarcinoma) and BSC - 1 (African green monkey kidney epithelial) cell lines as well as PMK (primary rhesus monkey kidney) cells are routinely maintained at the Viral and Rickettsial Disease Laboratory (VRDL) branch of the California Department of Public Health.

Only a few laboratories in the world are attempting this technique in human stem cell research and, thus far, no human stem cell lines have been derived using this method.

Frozen but hardly halted, the lines of reprogrammed stem cells in the Massachusetts General Hospital laboratory of biologist Konrad Hochedlinger are preserved in liquid nitrogen at -170 °C.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

Reprogramming adult cells to function like embryonic stem cells is one way researchers hope to create patient - specific cell lines to regenerate tissue or to study specific diseases in the laboratory.

The researchers established cell lines from the original egg and in seven out of eight cases the amount of mutated mitochondrial DNA remained low when the cells were grown in the laboratory.

Reagents: antibodies, plasmids, cell lines, RNA libraries, and more Organisms and viruses: model organisms of disease, mouse models, and more Biological specimens Software Protocols Core laboratories eagle - i is different from traditional web publications that list resources (websites, journals, etc.) because the tool allows researchers to: Share resources

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest.

In addition, Dr. Reinholdt's laboratory developed a novel protocol for derivation of embryonic stem cell line from a variety of strains that were previously deemed recalcitrant after years of failed attempts by the scientific community.

«Basically, this study shows that the genetic makeup of individual human embryonic stem cell lines is unique in the numbers of copies of certain genes that may control traits and things like disease susceptibility,» said Teitell, who also is an associate professor of pathology and laboratory medicine and a researcher at UCLA's Jonsson Comprehensive Cancer Center.

Cell lines can be frozen and shared between laboratories.

The laboratory, in the project I - Stem, received the first approval by the Biomedicine Agency, for importation into France of hES cell lines from abroad in accordance with current legislative framework.

In our laboratory, we have previously described an in vitro model of spontaneous transformation of the IL -3-dependent hematopoietic BaF3 cell line towards growth factor - independent tumorigenic clones with constitutive STAT5 activation.14 This model was developed from the study of BaF3 cells transfected with an IL - 9Rα mutant lacking the STAT - recruiting site (BaF3 phe116).

I - Stem's biobank contains a total of 38 hES cell lines covering 14 monogenic diseases: 19 were imported from foreign laboratories and 19 were derived in France (Tropel et al, 2010).

The metadata also details cryopreserved cells and cell lines that can be used to study the molecular effects of low oxygen conditions in the laboratory

On the contrary, recent evidence indicates that XMRV is a contaminant originating from the recombination of two mouse endogenous retroviruses during passaging of a prostate tumor xenograft (CWR22) in mice, generating laboratory - derived cell lines that are XMRV - infected.

The LNCaP cell line, in turn, had been likely infected with XMRV from 22Rv1 cells in the same laboratory in which 22Rv1 cells were previously used, or in another laboratory at the Cleveland Clinic that was working with both cell lines and had initially provided the LNCaP cells for analysis.

Once established, a cell line can be grown in the laboratory indefinitely and cells may be frozen for storage or distribution to other researchers.

Cells from these embryos can be used to create pluripotent stem cell «lines» — cell cultures that can be grown indefinitely in the laboratory.

Based on this analysis, it was mistakenly deduced that XMRV could not have originated from LNCaP or another cell line in the laboratory.

In the future, scientists may be able to modify human stem cell lines in the laboratory by using gene therapy or other techniques to overcome this immune rejection.