While Senate Republican leader Dean Skelos said Cuomo's denouncement would make negotiations on some (likely already dead) issues more «challenging,» many of his colleagues
expressed confidence that now Cuomo has secured the W.F.P. line — and kept Zephyr Teachout, a potential
left -
flank challenger, off the ballot — he'll revert to his normal centrist self.
When a Mir strain is combined with a Flp recombinase -
expressing strain, the lacZ and neomycin genes are removed
leaving an FRT site and the loxP -
flanked Mir stem loop.