Sentences with phrase «lentiviral construct»

A self - inactivating lentiviral construct containing a mouse phosphoglycerate kinase - 1 internal promoter driving the human gene encoding GDNF [44] was used to generate GDNF - secreting hNPCctx.

HEK293T cells were transfected with the following retroviral or lentiviral constructs, with their packaging vectors indicated in the parentheses, using FuGENE 6 reagent (Promega): pMX - puro - STAT5A * (provided by T. Kitamura, University of Tokyo; pCMV - Gag - Pol and pCMV - VSV - G); pLenti - EF - FH - TAZ - S89A (psPAX2 and pVSV - G); PLKO - NS and shTAZ lentiviral construct (provided by C. Linardic, Duke University; pCMV - Rev, pCVM - VSVG; pMDL).

Not exact matches

Charles Gersbach's lab has deposited mammalian expression constructs including pcDNA - dCas9 - p300 Core and pcDNA3.3 - Nm - dCas9 - p300 Core, as well as pLV - dCas9 - p300 - P2A - PuroR for lentiviral expression.

Prior to transplantation, we analyzed the in vitro production of specific growth factors by unmodified hNPCctx and by hNPCctx transduced with a lentiviral gene construct designed to constitutively express GDNF (hNPCctx - GDNF)[11], [44].

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

Entry vector constructs were recombined into adenoviral (pAd, Invitrogen) or lentiviral (pCSC - Zeo with Gateway recombination sites cloned into the multiple cloning site) destination vectors according to manufacturer's instructions.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS