Sentences with phrase «lentiviral vectors for»

Trastuzumab heavy and light chains were reformatted into a bicistronic expression cassette and cloned into adenoviral and lentiviral vectors for transient and stable expression, respectively.

Researchers have also developed integrase - deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for which transient transgene expression is sufficient.

The study also examines the use of lentiviral vectors for cell delivery in treating liver diseases, a tool traditionally used in treating blood disorders.

Not exact matches

Lentiviral expression vectors are the most effective vehicles for delivering genetic material to almost any mammalian cell

«The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.»

VCN and RCL Testing for Lentiviral Vectors Lindsay Skrdlant, PhD, Stanford University School of Medicine, United States

Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the pre - clinical / early clinical stages.

for applications that require the large genetic capacity of a lentiviral vector but for which transient transgene expression is sufficient.

But what's more, the transplanted cells keep producing a foreign protein slipped inside with the help of a lentiviral vector, the kind usually used for gene therapy.

Clinical benefits of lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic disorders

Although adeno - associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular for targeting and tracing cells in the brain (Figure 1).

Since lentiviral vectors can deliver a large amount of DNA (~ 8 kb) with a relatively low immune response, it should come as no surprise that researchers are interested in developing these vectors for gene therapy.

Verma has successfully developed lentiviral vector - mediated mouse models for glioblastoma, lung adenocarcinoma and small cell lung cancer (SCLC).

Genes associated with melanoma are overexpressed or silenced with shRNA constructs in lentiviral vectors and the lab increasingly uses cDNA and sh (short hairpin) RNA libraries for our experiments.

Because of the limitations of conventional laboratory - based culture methods and an increasing demand for lentiviral vectors, newer, larger - scale production methods are increasingly used.

The LVSCID clinical trial for X-linked severe combined immunodeficiency uses a lentiviral vector to insert a healthy copy of the IL2RG gene into blood cells.

Adenoviral vectors can be produced by human embryonic kidney (HEK293) and human embryonic retinal (PER.C6) cell lines (31), both of which also can be used for lentiviral vector production.

Tags for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression, Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS