Sentences with phrase «levels of a single gene»

«But it's remarkable that lowering levels of a single gene could have such a strong effect on the disease.

Not exact matches

Rare mutations that shut down a single gene are linked to lower cholesterol levels and a 50 percent reduction in the risk of heart attack, according to new research from Washington University School of Medicine in St. Louis, the Broad Institute at Massachusetts Institute of Technology and Harvard, and other institutions.

Just 11 weeks after getting a single shot of the gene therapy, 10 young mice lacking the ob / ob gene reached weight, insulin, glucose, and leptin levels comparable to normal mice.

However, cancer cells may instead be coaxed to turn back into normal tissue simply by reactivating a single gene, according to a study that found that restoring normal levels of a human colorectal cancer gene in mice stopped tumor growth and re-established normal intestinal function within only 4 days.

RNA sequencing of both single and clustered CTCs from breast cancer patients identified several genes expressed at elevated levels in CTC clusters, one of which — a protein called plakoglobin — also was overexpressed in the primary tumors of patients with reduced survival.

The team of medical and engineering researchers at The Ohio State University previously determined that modifying a single gene to reduce this protein's level in breast cancer cells lowered the cells» ability to migrate away from the tumor site.

Once again, they saw that methylation of a single spot in the serotonin transporter gene was associated with lower levels of serotonin transporter expression in the amygdala.

At the core of this cell behavior is how the loss of that single gene changes activation levels of dozens of other genes, suppressing genes associated with metastatic disease and increasing activity of genes linked to normal tissue.

When a single gene exists in the ancestral mutation, that would have made for higher levels of enzyme expression, would have messed up with sensory function.

After analyzing brain tissue samples, the researchers identified an area of the gene where a single change reduced BDNF levels in the hypothalamus, a key area that controls eating and body weight.

The team found that adding in a single extra copy of gene that expresses Maf1 decreased stored lipids by 34 percent, while reducing Maf1 levels increased stored lipids by 94 percent.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

At the cellular level, although they share canonical types of inhibitory interneurons (INs) and excitatory principal neurons (PNs), it remains largely unknown to what extent a single type in different brain regions displays similarity in gene expression, axonal shape, connectivity, and developmental origins.

«We showed at the single - cell level that the exchange of resistant genes is not influenced by antibiotics at all, which is in contrast to the literature.»

While the disease is caused by a single gene mutation, the manifestation of the disease at a molecular level is nevertheless highly complex and involves multiple cellular processes.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

Finally, the study provides an example of a highly integrative approach to understanding brain function at multiple scales, «linking genes and gene networks to the properties of single neurons and populations of neuron subtypes, all the way up to the level of animal behaviors,» said Okaty.

A single infusion of the gene therapy drug showed improved levels of the essential blood clotting protein Factor VIII, with 85 per cent of patients achieving normal or near - normal Factor VIII levels even many months after treatment.

Most importantly, by looking at a single cell level, researchers were able to correlate the chromosomal make - up of an embryo to a subset of 12 genes that are activated prior to the first cell division.

Promoting survival and proliferation at the single - cell level is critical for expansion of any clonal colonies containing the mutation of interest generated by the gene editing process.

The method, PrediXcan, estimates gene expression levels across the whole genome — a better measure of biological action than single mutations — and integrates it with genome - wide association study (GWAS) data.

Cas9 nucleases from Streptococcus pyogenes (SpCas9) have been widely used for both gene knockout and knock - in at the level of single or multiple genes.

Our supermatrix expands upon these previous studies by bringing together sequences from a wide array of molecular studies, supplemented by new data for four genes, to yield a single primate phylogeny with strong support for the monophyly of most primate genera, families, and higher - level taxa (Primates, Strepsirrhini, Lemuriformes, Lorisiformes, Haplorhini, Tarsiiformes, Simiiformes, Catarrhini, Platyrrhini).

To directly measure the level of coordination in the expression of genes, we used highly sensitive fluorescence in situ hybridization (FISH) to count individual mRNAs of functionally related and unrelated genes within single Saccharomyces cerevisiae cells.

His group work on understanding the regulation of gene expression, especially at the single - cell level where he has pioneered analytical approaches.

Home > Press > Single - cell mRNA cytometry via sequence - specific nanoparticle clustering and trapping: Cell - to - cell variation in gene expression creates a need for techniques that can characterize expression at the level of individual cells

Random fluctuations in transcription are unavoidable and a general aspect of life at the single - cell level and lead to «noise» around the average level of gene expression.

Furthermore, the levels of transcripts of two AlkB genes that protect against the cytotoxicity of methylating agents through repair of the specific DNA lesions generated in single - stranded DNA, Alkhb3 (alkylation repair homolog 3) and Alkhb8 (alkylation repair homolog 8), was lower in the IVC males.

The present study shows that such a gradient is also seen at the level of single cell analysis, but reveals that there is considerable cell - to - cell variation within this gradient in the expression of pluripotency genes.

To confirm the accuracy, fidelity, and sensitivity of the method, we carried out several tests including the dilution of a pool of cDNA to levels equivalent to single cell content prior to measurement of gene expression in ten replicates.

Thus, as anticipated from earlier studies, heterogeneity of pluripotency gene expression in single cells is to a degree reflected at the protein level.