Sentences with phrase «library preparation»

After library preparation of the samples, 55 of the total 65 had enough DNA to be indexed, or «bar - coded», and amplified for sequencing.
The experimental workflow includes library preparation, cluster generation and sequencing.
The SmartChip platform can be used for profiling and validating molecular biomarkers, and can perform massively parallel singleplex PCR for one - step target enrichment as well as library preparation for clinical NGS.
Services include global and gene - specific DNA methylation analysis, and chromatin immunoprecipitation (ChIP) followed by library preparation in a high - throughput format (HT ChIP - Seq).
The UniqSeq ChIP library preparation kit is a highly sensitive kit especially for low abundant transcription factors, and can be used for both qPCR and sequencing.
This unique technology was launched in December 2017, and integrates automated chromatin immunoprecipitation and next generation sequencing library preparation within the same protocol, eliminating the traditional tedious steps, purifications, and inefficiencies that can result from standard ChIP - seq protocols.
These improvements push the boundaries of nucleic - acid detection and fragment sizing, affecting applications such as single - cell nucleic acid analysis, PCR - free preparation of NGS library preparation, bacterial artificial chromosome clone sizing, and exosome analysis.
We evaluated four independent library preparation methods and two sequencing platforms (9)(table S1) for our first batch of 15 inactivated EVD samples from 12 patients.
The Apollo 324 library preparation system can be used to process DNA and RNA from clinical samples to NGS - ready libraries.
The TMWG kit incorporates Cambridge Epigenetix's TrueMethyl oxidative bisulfite (oxBS) technology, which allows researchers to accurately quantify different DNA modifications at single - base resolution, as well as proprietary library preparation methods designed to overcome the limitations of traditional bisulfite library construction, and to improve the yield and quality of epigenetic data.
MagSi - NGSPREP supports all standard DNA clean - up protocols encountered during Next - Gen library preparation
This simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument.
A comprehensive range of gDNA and amplicon library preparation kits and protocols is available, offering high throughput, low DNA input, rapid preparation and ultra-long reads.
High quality library preparation is key to a successful ChIP - Seq experiment.
Simultaneous detection of DNA and RNA virus species involved in bovine respiratory disease by PCR - free rapid tagmentation - based library preparation and MinION nanopore sequencing
The Applications team at Oxford Nanopore has two overarching responsibilities: creation and development of sample and library preparation protocols for a wide variety of sample types, and undertaking biological projects which highlight the various strengths of Oxford Nanopore's technology.
Introduction to ONT devices and latest technology Wet lab training and best practices for sample quality and library preparation for Nanopore sequencing Running MinKNOW and real - time sequencing data handling Introduction to basecalling and analysis tools (ONT and opens source) for analysis of ONT data
The Illumina library preparation and sequencing was done by the Cornell Biotechnology Resource Center.
The Gemcode system consists of a small desk - top instrument in a microfluidistic format and a set of 750000 barcodes for sequencing library preparation.
The technique, which they are calling a single - stranded library preparation, involves stripping the genetic material down to individual strands to copy and avoids a purification step, which can lose precious genetic material.
In gene - baiting, scientists use targeted PCR primers to amplify specific genes during the library preparation step, rather than amplifying all of the DNA fragments in the sample.
This kit requires a clean - up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.

Phrases with «library preparation»

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