Sentences with phrase «lif e»

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LIF is often used for ES cells and a special type of iPS cell.

We are particularly interested in the role of the transcription factor Nanog, which we isolated through a functional screen for molecules capable of directing ES cell self - renewal in the absence of the otherwise obligatory LIF signal.

In addition, their independence of LIF for iPS cell culture provides an opportunity to further understand the ground state of ES cell self - renewal proposed by Ying et al. [21].

Our unpublished data show that HFF cells can support derivation and culture of mouse ES cells in an undifferentiated state without exogenous LIF for more than 30 passages.

In contrast, the same mouse ES cells cultured on MEF cells began to differentiate after 2 passages in the absence of LIF (data not shown).

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

In vitro, ES cells can generate PrEn - like cells either in response to LIF withdrawal [24] or through forced expression of the transcription factors Gata4 or Gata6 [25], [26].

Nanog overexpressing and control cell lines were cultured in the absence of LIF for 10 d and assessed for ES cell - like morphology.

In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].

(B) Nanog overexpression makes HV ES cells resistant to LIF withdrawal.

Briefly, ES cells were kept in standard ES cell medium containing leukemia inhibitory factor (LIF) which prevents cell differentiation.

For instance, the role of LIF - gp130 - Stat3 axis in promoting ES cell proliferation is particularly well - defined [1], [3], and BMP - induced differentiation signals are inhibited by a Nanog - Smad1 protein complex [4]; crosstalk between these pathways has also been reported [5].

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