Sentences with phrase «ligation steps»
Targeted re-sequencing often entails discrete amplification and ligation steps during sample preparation that increase both cost and time to results.
http://www.moleculardxeurope.com/ Not exact matches
Cloning of any DNA sequence involves the following four steps: amplification, ligation, transfection, and screening / selection.
Due to their small number of nucleotides, traditional polymerase chain reaction (PCR) detection methods must necessarily involve a ligation, or linking, step to produce longer complementary DNA strands.
A final ligation reaction correctly assembles the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker sequence into the targeted loci.
As owners step away from the bonds of politically correct serfdom and demand ligation and vasectomy, cost will go down, more veterinarians will study and perfect safe, hormone preserving sterilization and dogs will no longer be forced to suffer the adverse effects and cruelty of sexual castration.
The steps include evaluating legal options and remedies, conducting a preliminary investigation, sending continuing obligations or cease and desist letters to the former employee and new employer, exploring the advantages and disadvantages of pursuing litigation, and sending ligations hold notices.