During his PhD he worked on three main projects: digital scanned laser
light sheet fluorescence microscopy (with Dr. Ernst Stelzer), the in toto reconstruction of zebrafish embryogenesis (with Dr. Jochen Wittbrodt), and the computational analysis of the evolution of the yeast genome architecture (with Dr. Michael Knop).
Modern
light sheet fluorescence microscopy was first pioneered by Voie and colleagues and originally named orthogonal - plane fluorescence optical sectioning (OPFOS)(2).
Alpha3 is a new generation of
light sheet fluorescence microscopes providing unrivaled imaging performance for fixed and live specimen.
Using this approach, it takes about an hour to scan a piece of tissue 500 by 500 by 200 microns, using
a light sheet fluorescence microscope.
Acquiring images using modern techniques such as
light sheet fluorescence, confocal, or electron microscopy creates a significant data stream.
Not exact matches
Scientists around the world employ commercial versions of diSPIM, which uses a thin
sheet of
light and two objectives lenses to excite and detect
fluorescence.
An ultrathin structured
light sheet (blue - green, center) excites
fluorescence (orange) in successive planes as it sweeps through a specimen (gray) to generate a 3D image.
These include custom - built
light sheet microscopy for single molecule and whole embryo measurements, laser ablation to perturb and dissect the architecture of microtubule structures, quantitative polarization microscopy, and new
fluorescence correlation spectroscopy approaches.
In 2015
light sheet - based
fluorescence microscopy was honoured as «Method of the Year 2014» by Nature Methods.
Since last year, LUXENDO is a part of the BRUKER corporation, adding
light sheet microscopy to BRUKERs
fluorescence microscopy branch.
UltraMicroscope II utilizes six
light sheets to excite large samples with
fluorescence light.
Overcoming the limitations of confocal
fluorescence microscopy, we invented and developed
light sheet - based
fluorescence microscopy (LSFM) establishing it as a major tool in the modern life sciences.
Fully developed and drug - affected several hundred µm thick spheroids are not very dynamic but require confocal and
light sheet - based
fluorescence microscopy for a full analysis of the spatial distribution of several fluorescing targets.
A guide to
light -
sheet fluorescence microscopy for multiscale imaging.