Sentences with phrase «like cells in culture»

A new study in Nature Genetics identifies a specific population of pluripotent embryonic stem cells that can reprogram to totipotent - like cells in culture.

Not exact matches

Animal cells are cultured from stem cells and incubated in a «bioreactor» into tissue that can be «harvested» and formed into familiar foods like meatballs, patties, and fish sticks.

While some still object to cells being taken from animals and used by scientists to grow clean meat in laboratories — and some just don't like the idea of eating a «cultured» steak created by men and women in white coats — others see the lab - grown meat revolution as key to solving the environmental crisis linked to meat eating.

For instance, to make a rod with collagen fibers aligned along its length (like a tendon) they cultured chondrocyte cells in a dog bone - shaped mold with loops on either end.

When these cultured cells were exposed to an air / liquid interface in the laboratory, they stratified, generating what the authors referred to as a multi-layered, «skin - like organoid.»

Finally, in cell cultures and animals, overexpressing the hormone could produce something that looks like cancer, and that would shrink when treated with an IGF - 1R blocker.

Droplet - based microfluidics is a tool used in various applications like cell cultures, chemical synthesis and DNA sequencing.

Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.

The cells were put into a special culture and then suspended in a gel and stimulated by nutrients, all geared to turn them into neurons like those found in the cortex.

In the study, the researchers show that after two to four weeks of 3 - D culture, the resulting muscle cells form muscle fibers that contract and react to external stimuli such as electrical pulses and biochemical signals mimicking neuronal inputs just like native muscle tissue.

In our platform, cells looked and acted like those that you would find in the body, showing more sensitive responses to drugs than traditional static culturing.&raquIn our platform, cells looked and acted like those that you would find in the body, showing more sensitive responses to drugs than traditional static culturing.&raquin the body, showing more sensitive responses to drugs than traditional static culturing.»

What about the rare stem - like cells generated in cultures of ovarian cells?

«We culture our cancer cells in this very low - oxygen environment, and they start behaving like they are inside a low - oxygen tumour.»

Rather than stick the isolated cells on a flat culture dish, they mixed the cells with liquid, then let the mixture hang in tiny droplets from a plastic lid, like condensation on the roof of a container.

To determine whether endothelial cells — the cells that line the interior surface of blood vessels — directly influence breast cancer cell growth, they then created unique organotypic models of lung and bone marrow microvascular niches, in which endothelial cells formed blood vessel - like structures in culture as they would in the original organ.

After experimenting with several different cocktails, the researchers hit on a mix that transformed 100 percent of cultured stem cells into those that looked like neurons in under an hour.

The team then cultured the cells for several months in a bath of growth - promoting proteins, trying to get the cells to reverse course and become more like stem cells.

Their stem cells, which can mature into any type of tissue, were isolated and cultured in a dish around dots of gel - like growth medium.

By comparing hippocampal and cortical neurons in mono - culture (with other cells of the same type) and in co-culture (both cell types deposited within one device), the research showed that some features from hippocampal cells, like the percentage of action potential spikes found in bursts, were significantly higher when cultured together with cortical cells.

«Using this cell line allows us to separate 2 - cell - like cells from the ES cells in the culture by collecting the green cells which have entered the 2 - cell like state.

Next, the researchers would like to investigate how to culture the other types of progenitor cells that are required for a full kidney, in addition to the nephrons formed by NPCs.

To see if they would indeed behave like heart cells, they mixed some of these in with cultures of heart cells from newborn rats.

When the researchers deliberately blocked the gene's activity in cultured breast cells by adding RNA sequences that bound to BRCA1 mRNA, preventing it making protein, they found that the cells began to divide rapidly, just like malignant cells.

Ralph Brinster, part of the team at the University of Pennsylvania in Philadelphia that first cultured sperm stem cells in the lab, has written that culturing stem cells from human sperm is not far off — humans and mice, like other mammals, he says, require similar growth factors.

Scientists at the University of Luxembourg have succeeded in turning human stem cells derived from skin samples into tiny, 3 - D, brain - like cultures that behave very similarly to cells in the human midbrain.

Forced expression of this SMN1 variant in cultured non-neuronal cells induces the formation of neurite - like extensions, a change in cell shape reminiscent of that occurring when motor neurons send out axons to their muscle targets.

These cells produced platelet - like particles (PLPs) similar to those isolated from human peripheral blood following an additional 18 days of culture in a chemically defined media without feeder cells.

Laminin cultured cells arrange themselves in lobule like structures, express MRP1 and MRP2 and are capable of biliary efflux and show significant effect on hepatocyte P450 enzyme metabolic activity.

Our team would like to be able to research passenger pigeon genes for de-extinction while primordial germ cell cultures are developed, and an Australian team has developed an effective way of bypassing cell cultures for engineering birds by going directly to the primordial germ cells in the embryo.

Because neurodegenerative disorders like PD are largely diseases of aging, modeling them in a culture dish using neurons grown from iPS cells has been thought to be exceedingly difficult, if not impossible.

La Jolla, CA — Cells cultured in the lab are like a fish out of water.

In short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culturIn short, there is no blastocyst - like stage — when the somatic cells are reprogrammed, they're immediately cultured as cells in normal adherent culturin normal adherent culture.

Furthermore, we found that the stem - like and EMT phenotypes observed in the cycling hypoxia - selected subpopulation are not reversible, because we obtained the same molecular profile from this subpopulation by culturing them as spherical cultures for several months in normal oxygen content and culturing them as adherent cells (Figure S2 in Additional file 2).

In a curious twist resembling cellular alchemy, when blood T cells are cultured with lymphoid tissue from the tonsils or spleen, the blood T cells begin acting like lymphoid tissue T cells, becoming more activated and more susceptible to abortive infection and death by pyroptosis.

By activating a gene called Ras in cells bathed in a very specific culture medium, scientists were able to cause embryonic stem cells — which originate from the inner cell mass of the blastocyst — to become more like the trophoblastic stem cells that give rise to the placenta from the outer portion of the blastocyst.

«Excitingly, the program has identified drug - like compounds that act on SMA patient cells in culture to increase SMN2 gene activity and thereby the amount of functional SMN protein.

(C) When iPS cells were cultured in the absence of the HFF cells, various differentiated cells could be observed, such as definitive endoderm - like cells (a) and (b), fibroblast - like cells (c), cardiomyocytes (d), epidermis - like cells (e) and neural rosettes (f).

Kato et al. «Establishment of An Osteoid Preosteocyte - Like Cell MLO - A5 That Spontaneously Mineralizes in Culture», Journal of Bone and Mineral Research, 16 (9): 1622 - 1633, 2001.

Nanog overexpressing and control cell lines were cultured in the absence of LIF for 10 d and assessed for ES cell - like morphology.

Note the brighter intensity of Venus in the tightly apposed pavement - like cells in the LIF negative culture (white arrows).

While Hex is discretely expressed in the VE on the anterior side of the embryo, it is initially expressed throughout the early PrEn [28] and like the GATA factors, Hex transcripts are also detectable in some ES cell cultures [29].

«We identified chemical compounds that have drug - like qualities and effectively lower tau levels in cultured brain cells,» explained Mucke, who is also a professor of neuroscience and neurology at UC San Francisco.

Of great interest, stem - like cells have also been discovered in some cultured cancer cell lines, including glioma (22, 23), ovarian (24, 25), liver (26), and prostate (27 — 29) cancer cells.

The cells are called lung spheroid cells for the distinctive sphere - like structures they form in culture.

Interestingly, long - term — cultured human epithelial cancer cells in clonal cultures also form holoclones, meroclones, and paraclones, and tumor cell holoclones have been hypothesized to harbor stem - like cells or cancer stem cells.

During the culture of these cells, we noticed a change in cell morphology from the cobble stone - like shape typical of epithelial cells to a more spindle - like and scattered appearance (Figure 2A, lower panel), indicating these cells may be undergoing EMT.

Appealing to those who are horrified by the new culture, he offers as an analogy Aldous Huxley's Brave New World or, worse, the world of E. M. Forster (from «The Machine Stops,» published in 1909): «Imagine, if you can, a small room, hexagonal in shape, like the cell of a bee.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)