These approaches, which include exotic sounding tools
like zinc finger nucleases and CRISPR / Cas9, differ from more traditional ways reducing the impact of the HD mutation on cells.
In the past, researchers have attempted to do this with gene editing tools
like zinc fingers nucleases, but the process was too labor - intensive to be used on a large scale.
Not exact matches
«Before CRISPR, there was TALENS [transcription activator -
like effector
nucleases] and
zinc finger nucleases — older technologies that were not as precise or reliable,» explains Sosa.
Ultimately, this week's discourse will lead to a consensus statement providing some guidance on how to approach using this and older gene editing technologies such as
zinc finger nucleases and enzymes called transcription activator -
like effector
nucleases, or TALENs.
The variety of new tools available for genetic manipulation now include lentiviral - based gene delivery, and gene editing using CRISPR / Cas9,
zinc finger nucleases (ZFNs) or transcription activator -
like effector
nucleases (TALENs).
Zinc finger nucleases [1], [2], transcription activator -
like effector
nucleases [3], [4] and homing meganucleases [5] have provided powerful tools to induce targeted mutations in the form of small insertions or deletions derived from DNA break repair of nonhomologous end joining (NHEJ) or homologous recombination.