The lower diagnostic specificity of this qPCR assay may be the result of the lower
limit of detection of this assay compared with the gold standard rather than the result of detection of true false - positives.
The Company's T2 Magnetic Resonance platform (T2MR) enables detection of pathogens, biomarkers and other abnormalities in a range of unpurified patient sample types, including whole blood, plasma, serum, saliva, sputum and urine, and can detect cellular targets
at limits of detection as one colony forming unit per milliliter (CFU / mL).
The optical density of samples did not increase linearly, so a higher concentration of hemoglobin defines the
upper limit of detection for the device.
We have studied a total of 170 samples from chronic fatigue syndrome patients from two UK cohorts and 395 controls for evidence of XMRV infection by looking either for the presence of viral nucleic acids using quantitative PCR (
limit of detection < 16 viral copies) or for the presence of serological responses using a virus neutralisation assay.
If you are getting unexpected low values when the instrument is working at the
very limit of its detection, then you put that aside saying maybe there is something happening there that is real, but maybe it is an instrumental problem, and we'll have to go back and look at it carefully.»
When these methods have been applied on Earth they have enabled scientists to
lower limits of detection or to better understand formerly ambiguous observations.
By probit analysis, the 95 %
limit of detection for TMAdV was 781, 377, or 35 viral genome equivalents / mL for serum, stool, or oral swab samples, respectively (data not shown).
If there are any biting females, they are not expected to transmit the glowing - red protein or the tetracycline protein: «concentrations of these proteins in the saliva of [Oxitec] females are below
the limit of detection.»
The assay was found to be 100 % sensitive and 100 % specific to ZIKV RNA from semen samples with
a limit of detection of 5 viral copies per mL of semen.
«The amount of viral genetic material in the placentas and fetuses from the vaccinated females was just above
the limit of detection,» said Diamond, who also is a professor of molecular microbiology, and of pathology and immunology.
A single troponin T concentration below
the limit of detection in combination with a nonischemic electrocardiogram (EKG) means that MI is unlikely and patients can be safely discharged.
He doesn't not think
the limit of detection was 50-fold lower than what Wolfe - Simon found, as Redfield claims.
«We can do fancier analyses that push
the limits of detection down, but I think the burden of proof is back on the authors,» she says.
High - sensitivity troponin I concentrations that were below
the limit of detection identified 19 percent of patients as being potentially suitable for immediate discharge, with a high diagnostic performance in excluding AMI.
We also demonstrate that cutoff values above the lower
limit of detection may not have the required diagnostic performance for clinical implementation,» the authors write.
The lower
limit of detection (1.2 - ng / L) as well as rounded cutoff concentrations for a high - sensitivity troponin I assay were used in the analysis.
«We're pushing
the limits of detection,» says Chun.
Goordial adds, «We don't know if there is activity beyond
our limits of detection.
Advances in technology and analytical chemistry have lowered
the limits of detection, allowing for the detection of these chemicals at concentrations as low as parts per billion and parts per trillion.
In project DINAFF, researchers at INM and Superon GmbH managed to reduce the loss of nanoparticles during analysis and, therefore, to improve
the limit of detection.
Some HIV - positive individuals have been able to maintain virus load levels below
the limits of detection for 30 years or longer.
10 of 19 (53 %) placentas and 11 of 19 (58 %) fetal heads from mice who received Moderna's Zika mRNA vaccine had viral RNA levels at
the limit of detection of the assay, suggesting virtually complete protection, and the remainder had substantially lower levels than those detected in samples from mice in the placebo cohort.
For example what was
the limit of detection and limit of quantification of the methods used?
«In summary, whilst effects are reported on individually measured forager parameters, the associated reported residues of thiamethoxam were very low (probably close to
limits of detection) and there was no demonstration of a dose response effect across the modelled exposure gradient.
The limit of detection by testing 10 fold - dilutions normal human plasma and L - 15 growth medium for AP61 cell was the same.
Limit of detection is 38.29 (PSP) and 34.04 (MO BIO).
In this population, 12.3 % of patients had baseline and 1 - hour hsTnI values below
the limit of detection (2 ng / L), with an NPV of 100 % for MI.
Diagnostic Accuracy of
the Limit of Detection and Rounded Low Cutoff Concentrations of High - Sensitivity Troponin I (hs - cTnI) With Nonischemic Electrocardiogram
(Note that, as with any assay, when we indicate that a protein was «not found» or «not identified» that is defined as within
the limits of detection).
Recent testing by Eurofins in Germany also found white mighty rice ® to be below
their limit of detection (< 1 ppb).
I found some rice that claims to be arsenic free because they are below Darthmouth college and Eurofin's
limit of detection.
A spokesperson from the HPA confirmed that the quantities involved were «at
the limits of detection».
The lower
limit of detection was 1.0 nmol / l.