Sentences with phrase «line with a gene»

Overall, making a human cell line with a gene knockout was straightforward, and is a testament to how far genome engineering has come.

Not exact matches

A poem by the American poet Gene Derwood (l909 - l954), titled «With God Conversing,» contains these two lines: «The gloomy silhouettes of wings we forged / With reason reasonless, are now enlarged.»

If genes determine our mating preferences, how is it that these supposedly hardwired instincts erode in line with societies» and individual's gender - egalitarianism?

But mice with a working version of the gene suffered little to no damage to their gut - lining cells.

Nine lines of Cavendish Grand Nain transformed with the nematode - derived Ced9 gene were also trialled, with one line remaining TR4 - free for the three years

For the study, the researchers created a line of zebrafish with defective immune systems by inserting a mutation into a gene required for development of an important component of the immune system.

They developed experimental lines with and without the high protein gene by breeding the gene into two varieties of soybean and testing the lines for both protein concentration and yield.

So even if you start off [with] say, a resistant type of staph infection, that line of staph organisms not only, will they be more resistant, but they may pass some of the genes for that resistance off to completely other unrelated types of bacteria.

They first tried to reconstitute the system by working with one or two genes at a time, but this «assembly line» approach just didn't work.

For this study, they created several lines of transgenic Brazilian and African rice that overexpress this gene, and with their CIAT and JIRCAS collaborators, tested how well the rice grew in different conditions in different years.

The drug killed mutated strains with those genes knocked out faster than it killed the normal strain (solid lines), even though the three strains grew similarly without the drug (dashed lines).

Gene editing could include altering genes in one person — say to treat leukemia in one patient or make a cosmetic change — but, more controversially, it could also include making changes to the germ line that would then alter the genome for an individual's children, grandchildren and the following generations, with potentially unknown repercussions.

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

The researchers treated 63 cancer cell lines (26 breast, 14 colorectal and 23 ovarian) with low - dose 5 - azacitidine (AZA), an FDA - approved drug for myelodysplastic syndrome, that reverses epigenetic changes by stripping off the methyl group that silences the gene.

With help from researchers at Duke University, they showed that the gene has a unique pattern of methylation in breast cancer cell lines that might one day help doctors spot the disease.

In line with the views of most biomedical researchers, lawmakers struck a note of caution about the implications of new gene editing techniques that make heritable changes to human embryos.

One of them was able to cause full - blown disease symptoms — tan spore - producing lesions on the leaves — on eight soybean lines, including two with multiple resistance genes.

«Nevertheless, this gene will be useful in combination with other STB resistance genes, and our study provides tools for rapid lab - based tracing of Stb6 in wheat breeding lines,» says Kanyuka.

Patients with metastatic colorectal cancer (mCRC) that are mutation - free in the KRAS, NRAS, BRAF and PIK3CA genes showed significant benefit from continuing anti-epidermal growth factor receptor (EGFR) therapy beyond progression following first - line chemotherapy and an anti-EGFR monoclonal antibody, according to study results (1) presented today at the ESMO 17th World Congress on Gastrointestinal Cancer in Barcelona, Spain.

They also created Cavendish lines with Ced9, a nematode gene known to confer resistance to many kinds of plant - killing fungi.

Biotechnologist James Dale and colleagues at Queensland University of Technology in Brisbane, Australia, cloned a resistance gene named RGA2 from a type of wild banana that's impervious to TR4 and inserted it into the Cavendish, creating six lines with varying numbers of RGA2 copies.

In line with this interpretation, the enzyme encoded by this gene also plays a role in producing hormones (thyroid and insulin) involved in brain development.

After exploiting a technology that allowed them to activate each of nearly 16,000 genes individually in human melanoma cell lines containing mutant BRAF, the authors then treated the panel of cells with the drugs and monitored which cells showed altered drug sensitivity.

However, Eggan and McCarroll emphasized that now that this phenomenon has been found, inexpensive gene - sequencing tests will allow researchers to identify and remove from the production line cell cultures with concerning mutations that might prove dangerous after transplantation.

Researchers have ignored these noncoding RNAs until recently for not complying with the central dogma of biology — that a straight line runs from gene to RNA (transcription) to protein (translation).

The first study to sequence and analyze the entire genome of a HeLa cell line, along with access to its sequence data, has been published Aug. 7 in its final version, by G3: Genes Genomes Genetics, an open - access, scientific journal of the Genetics Society of America.

In the lab, the scientific team used an approach that combined functional RNAi analysis with gene expression analysis in breast cancer - derived cell lines and in human breast cancers replicated in mice.

He and his team have studied LINE - 1 and other jumping genes for years, working with colleagues from the University of Pennsylvania and the Salk Institute, who are co-authors on this paper, and, in previous studies, with colleagues at Duke University.

In the jargon, they are building the mouse «connectome», named in line with the term «genome» for the sequence of all of an organism's genes, «proteome» for all its proteins, and so on.

In combination with a separate technique that measures the degree to which a gene is expressed, the researchers then identified genes that were either mutated or functionally altered through expression levels in these bladder cancer cell lines.

But other genetic data suggests that at least one high - elevation gene appeared in Tibetans only between 2750 and 5500 years ago — more in line with the appearance of high - elevation agriculture on the plateau.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

Professor Seymour said: «We already have a line harboring a very small section of the S. pennellii genome with the alternative form of the pectate lyase gene.

Regulatory debate Huang's team's April report spawned a flurry of scientific and policy meetings and statements as governments and policy experts wrestled with how or whether to draw the line on gene editing in human embryos.

Working with mouse prostate cancer cell lines in the laboratory, the investigators found that cells containing overexpressed TOP2A and EZH2 genes were highly sensitive to attack with a combination of two drugs.

That's about 2000 years earlier than suggested by previous studies focusing on a different, more selective set of genes known as the exome, but it's in line with recent archaeological findings that point to distinctly Tibetan permanent settlements appearing between 3600 and 5200 years ago, Yang says.

The dedicated tailoring enzymes are encoded by genes clustered with the assembly line genes for coordinated regulation.

«The bottom line is if you have a species with compatible relatives that occur in the same area, gene flow will occur,» she says.

Overall they found that many of these risk genes are associated with the myriad of functions taking place in cells lining blood vessels.

Deep sequencing analysis has shown that this is highly unlikely in the case of J3 as no sequences were generated by deep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gene.

Interestingly, A14, B9 and B21 GL VHH (with the V gene reverted to germ line but mature DJ regions) were all able to bind the R2 immunogen and neutralize the homologous virus (Fig. 7) but only B21 GL could bind the both immunogens and neutralize both viruses.

ReNeuron can generate large numbers of stable cell lines by engineering cells with a modified version of the gene c - myc.

These comprised the relevant germ line V gene paired with the mature CDR3 and J sequence of each VHH (Figure S3 in S1 Text).

The protein encoded by the PIK3C2G gene belongs to the phosphatidylinositol -4,5-bisphosphonate 3 - kinase (PI3K) family, which plays a critical role in cancer.28 Experimental evidence suggests that activation of PI3K signaling enhances production of COX - 2 and PGE2, which results in inhibition of apoptosis in colon cancer cell lines that can be restored with NSAID - mediated blockade of PI3K.29

However, further sequencing analysis established that these germ line chimera were not present at the earlier time point, as no sequences with the fully mature DJ regions paired to the relevant germ line V genes were detected at t = 54 whereas between 50 and 250 copies of such clones were present at t = 174.

GL VHH comprising inferred germ line V gene paired with the mature VHH CDR3 and J region were produced.

Z - Scores Within Primary Site: Similar to Z - Scores, but the reference population used to compute the median and MAD is all CCLE lines from the same lineage with data for that gene.

Phenotype data from 449 mutant alleles were acquired, representing 320 unique genes, of which half had no previous functional annotation and data from over 27,000 mice were captured, finding that 83 % of the mutant lines are phenodeviant, with 65 % demonstrating pleiotropy.

«We want to characterize each line of mice broadly with no assumptions about what the gene is or is not doing,» said IMPC Executive Director Mark Moore, Ph.D. «If you think of the function of a gene as a needle in a haystack, we're removing the haystack so you can see what the needle does.»

The scientists began with a line of mice in which the gene for the insulin receptor has been knocked out in endothelial cells only.