Sentences with phrase «lining cells control»

Not exact matches

The comprehensive product range includes industrial scales, terminals, load cells, weighing platforms and software from receiving raw materials through various manufacturing processes, in - line process control and end - of - line packaging control.

New cell growth is stimulated as oil is brought under control and fine lines and wrinkles are smoothed away.

Using an experimental FA cell line, they also found that cells without frataxin produced lower levels of ApoA - I compared to controls.

The researchers found that production of this mucus lining depends upon a single immune system regulator — the NLRP6 inflammasome — that controls mucus secretion by cells in the wall of the intestine, just like turning on a faucet.

The enzyme was tested in three different cell lineages: leukemia cells incapable of producing asparagine at normal levels (MOLT4); another line of leukemia cells, in this case capable of producing asparagine at normal levels (REH); and non-malignant cells, used as a control (HUVECs).

Another image in that same set, figure 6, appears as a cloned cell line but also shows up in a supplemental figure labeled as the control line.

This results in shifts in the structure of the brain as it forms and displacement of cells from where they're supposed to be, including those that line the ventricles and help control fluid flow.

However, some experts warn that it's far from clear how many holders of existing hES cell lines will be willing to relinquish even partial control of one of biomedicine's hottest commodities.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

He also inserted the disease - causing variation into one healthy cell line from a family member, as well as the cell line from the unrelated control.

Melton says his lab has iPS cell lines from people with diabetes — both type 1 and type 2, in which the β cells are not destroyed — and healthy controls.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

Dr. Bauman and her colleagues treated human head and neck cancer cells in the laboratory with varying doses of sulforaphane and a control, and compared them to normal, healthy cells that line the throat and mouth.

One surprising finding from the study co-led by Johns Hopkins, Ehret says, was that many of the new sites identified were near genes that are active in cells that line the inside of blood vessels, suggesting those cells are somehow involved directly in blood pressure control.

Following up on clues — including the fact that PMS is 56 percent heritable — the NIH researchers studied the genetic control of gene expression in cultured white blood cell lines from women with PMDD and controls.

In addition, progesterone boosted expression of several of these genes in controls, while estrogen decreased expression in cell lines derived from PMDD patients.

An analysis of all gene transcription in the cultured cell lines turned up a large gene complex in which gene expression differed conspicuously in cells from patients compared to controls.

One of the studies, by Johns Hopkins University scientists in collaboration with many other groups, turned up unexpected hints that biochemical signals controlling blood pressure may spring from within cells that line blood vessels themselves.

Its first target was the regulatory network involved in controlling the differentiation of THP - 1 cells, a line of human leukemia cells used in laboratory experiments.

Individual cultures of 3T3 (a murine fibroblast line) and endothelioma cells subjected to identical treatment were used for negative and positive controls, respectively.

For a positive control, nuclear extracts were prepared from a rat C6 glioblastoma cell line incubated in the presence of CoCl2 for 2 h. CoCl2 is known to increase HIF - 1α protein levels [55].

The goal of this protocol (written in French) is to describe the steps and quality controls to prepare new stable murine embryonic stem cells lines: ① either from wild type mouse embryos to introduce a mutation by genetic targeting or homologous recombination to get a genetically modified mouse line ② or from genetically modified mouse embryos with two aims:

Her aim is to understand, at the molecular level, the mechanisms that control communication between the brain, immune system, and blood vessels — with the ultimate goal of designing new therapies that slow, stop, or reverse the progression of a wide range of neurological disorders, such as MS. Recently, Dr. Akassoglou's lab identified how microglia — a type of immune cell that acts as the brain's first line of defense — are activated when fibrinogen enters the brain or spinal cord.

Can be used as a negative control for other p - BABE - puro derived HOS CD4 cell lines.

This advance will allow biomedical scientists to study the functioning of an individual's intestinal lining in a controlled microenvironment, where the lining can interact with immune cells, blood cells and drugs, said Robert Barrett, PhD, assistant professor of Medicine and research scientist at the Cedars - Sinai Board of Governors Regenerative Medicine Institute.

Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - SIGN

Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.

Stem cell researchers from UCLA used a high resolution technique to examine the genome, or total DNA content, of a pair of human embryonic stem cell lines and found that while both lines could form neurons, the lines had differences in the numbers of certain genes that could control such things as individual traits and disease susceptibility.

We are the home of the UK Stem Cell Bank which provides a world - leading resource of ethically - sourced, quality - controlled human embryonic and other stem cell lines for research applicatiCell Bank which provides a world - leading resource of ethically - sourced, quality - controlled human embryonic and other stem cell lines for research applicaticell lines for research applications.

Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg / 1x106 cells) used under the same conditions.

To explore the biological function of DDX3 on cell growth control, a colony formation assay was done in several tumor cell lines (HuH - 7, HCT116, and HeLa) and normal murine fibroblast cells (NIH 3T3) under the selection of G418.

«Basically, this study shows that the genetic makeup of individual human embryonic stem cell lines is unique in the numbers of copies of certain genes that may control traits and things like disease susceptibility,» said Teitell, who also is an associate professor of pathology and laboratory medicine and a researcher at UCLA's Jonsson Comprehensive Cancer Center.

«Human Embryonic Stem Cell Lines Differ In Genes That Could Control Disease Susceptibility.»

Most dramatically, in one cell line, tumor regeneration was blocked in all 10 treated animals, while tumors formed in all 10 animals treated with the control miRNAs.

Our combined in vitro and in vivo expertise shapes a broad portfolio of design (ES cell, CRISPR / Cas9, random DNA insertion), breeding and associated services to create and maintain the most relevant models for your studies.Rely our industry - leading germ line transmission, strict quality controls, fast turnaround and dedicated project managers to help you successfully reach your goals.

To evaluate whether ABL kinases might regulate the secretion of osteoblast - derived RANKL or OPG leading to osteoclast differentiation, we analyzed RANKL and OPG mRNA abundance in the murine osteoblast cell line 7F2 in response to conditioned medium from control and ABL1 / ABL2 knockdown breast cancer cells.

This is in accordance with previous reports that decitabine and 5 - azacytidine produce a marked synergistic effect in combination with suberoylanilide hydroxamic acid and romidepsin in T - lymphoma cell lines by modulating cell cycle arrest and apoptosis.26, 27 As a mechanism of action, KMT2D mutations of B - lymphoma cells promote malignant outgrowth by perturbing methylation of H3K4 that affect the JAK - STAT, Toll - like receptor, or B - cell receptor pathway.28, 29 Here our study indicated that dual treatment with chidamide and decitabine enhanced the interaction of KMT2D with the transcription factor PU.1, thereby inactivating the H3K4me - associated signaling pathway MAPK, which is constitutively activated in T - cell lymphoma.13, 30,31 The transcription factor PU.1 is involved in the development of all hematopoietic lineages32 and regulates lymphoid cell growth and transformation.33 Aberrant PU.1 expression promotes acute myeloid leukemia and is related to the pathogenesis of multiple myeloma via the MAPK pathway.34, 35 On the other hand, PU.1 is also shown to interact with chromatin remodeler and DNA methyltransferease to control hematopoiesis and suppress leukemia.36 Our data thus suggested that the combined action of chidamide and decitabine may interfere with the differentiation and / or viability of PTCL - NOS through a PU.1 - dependent gene expression program.

Whereas conditioned medium from ABL1 / ABL2 - depleted breast cancer cells did not affect RANKL abundance in osteoblasts compared with the cells treated with control conditioned medium (Fig. 5E), we found that conditioned medium from breast cancer cells lacking ABL kinases increased OPG abundance in the osteoblast cell line (Fig. 5F).

To determine DAXX binding sites in the PCa genome, specifically in the PC3 cell line (derived from bone metastasis of a human PCa case [19]-RRB-, we generated a stable shRNA knockdown (K / D) PC3 cell line and a control shRNA counterpart [Submitted], and performed a ChIP - Seq study.

As noted in the JAX ® Mice strain data sheets, the best control for a targeted mutant is the 129 strain used to produce the ES cell line from which the mutant was derived (Simpson et al. 1997; Threadgill et al. 1997).

Cells from STAT6 DN (a) or STAT6Y641W (b) stable cell lines, corresponding vector controls (V) or parental cells (P) were cultured under normal culture condition and harveCells from STAT6 DN (a) or STAT6Y641W (b) stable cell lines, corresponding vector controls (V) or parental cells (P) were cultured under normal culture condition and harvecells (P) were cultured under normal culture condition and harvested.

Expression of Venus and SSEA - 1 were quantitated by flow cytometry in two independent clonal lines and compared to both control and parental cells.

Nanog overexpressing and control cell lines were cultured in the absence of LIF for 10 d and assessed for ES cell - like morphology.

Our duty is to thaw hES cell lines, amplify them at low passages, subject them to thorough quality control assays before freezing them by vitrification.

To determine whether the increase in p - Smad1 / 5 levels were paralleled by an increase in neurite growth, SH - SY5Y cells were transfected with an miR - 181a inhibitor or a scrambled control and cultured for 72 h before neurite growth was examined using a modified line intercept method as previously described [18].

For p53 and p73, the positive control was human embryonal kidney cell line 293 (BD Pharmingen, San Diego, CA).

As an additional control we also tested the effect of amiodarone HCl on an immortal cell line 2102Ep cells [13].

However, like most vertebrate cell lines, target sequences are inserted randomly and stable mutation and expression of the transcript can not be controlled for.

ACTB mRNA expression levels in lymphoblastic lines and fibroblasts derived from affected individuals were decreased in comparison to those in control cells.

Our control iPSC derivation efficiency using the HUF1 line was very low, with only 1 iPSC line derived from 200,000 cells.