Sentences with phrase «liquid chromatography with»

Determination of morphine in urine by solid - phase immunoextraction and high - performance liquid chromatography with electrochemical detection.

Kehr, J.; Hu, X.J.; Yoshitake, T.; Scheller, D. Determination of the dopamine agonist rotigotine in microdialysates from the rat brain by microbore column liquid chromatography with electrochemical detection.

High - pressure liquid chromatography with ultraviolet - visible spectral analysis of toluene extracts of samples from two Cretaceous - Tertiary (K - T) boundary sites in New Zealand has revealed the presence of C60 at concentrations of 0.1 to 0.2 parts per million of the associated soot.

For products with As concentrations of more than 300 ng / g, a different lot number sample was purchased and tested using ICP - MS for total As and high - performance liquid chromatography with ICP - MS for As species.

They used Liquid chromatography — mass spectrometry (LC - MS), which combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry (MS) to isolate the chemical constituents of virgin coconut oil (VCO).

Not exact matches

After years of growing out strains of «Bhut Jolokia», tests with High Performance Liquid Chromatography revealed the heat level to be in excess of one million Scoville Heat Units.

I have no idea how their supposed heat scale was developed, but the only scientific way to measure capsaicin levels is with High Performance Liquid Chromatography.

With this labeling method, the intrinsically labeled Golden Rice β - carotene showed a protonated molecule of m / z of Mβc + H + = 536 + 1 (representing unlabeled β - carotene) and a range of isotopomers with the most abundant showing an enrichment of 9 deuterium at Menrich - βc = Mβc + H + + 9 mass units (Figure 4) as analyzed by using the liquid chromatography / mass spectrometry with a positive atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 + H + = 536 + 9 + 1 = m / z 546)(With this labeling method, the intrinsically labeled Golden Rice β - carotene showed a protonated molecule of m / z of Mβc + H + = 536 + 1 (representing unlabeled β - carotene) and a range of isotopomers with the most abundant showing an enrichment of 9 deuterium at Menrich - βc = Mβc + H + + 9 mass units (Figure 4) as analyzed by using the liquid chromatography / mass spectrometry with a positive atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 + H + = 536 + 9 + 1 = m / z 546)(with the most abundant showing an enrichment of 9 deuterium at Menrich - βc = Mβc + H + + 9 mass units (Figure 4) as analyzed by using the liquid chromatography / mass spectrometry with a positive atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 + H + = 536 + 9 + 1 = m / z 546)(with a positive atmospheric pressure chemical ionization interface (the total m / z was Mβc + 9 + H + = 536 + 9 + 1 = m / z 546)(23).

German's colleague Carlito Lebrilla decided to attempt the job with the university's new nanoflow liquid chromatography time - of - flight machine, which identifies molecules by measuring the time it takes them to ping around a tube that looks like a stovepipe.

The process involving liquid chromatography - electrospray ionization - tandem mass spectrometry relies on a vital step called «charge derivatization» or using a permanently charged reagent to selectively trap the estrogens and isolate them from the lipids and proteins that could interfere with estrogen detection, Schug said.

In theory, they can do this by separating the acids with high performance liquid chromatography, but results from this method need to be compared to analytical standards, which do not yet exist for the individual compounds.

The Xevo G2 - XS QToF can be integrated with Waters» chromatographic tools, such as ultra-performance liquid chromatography, for high throughput.

Metabolite analyses were done using liquid chromatography coupled with mass spectrometry.

Reversed - phase high - performance liquid chromatography (HPLC) with electrochemical detection was used to analyse ascorbic acid, based on the method of Iriyama et al. with modifications [29], [30].

In MudPIT, a complex mixture of proteins and peptides are separated using 2 - D liquid chromatography, and then the separate proteins are digested (chopped into smaller «peptide» pieces with enzymes) and subjected to the sensitive technique of mass spectrometry (which identifies the components based on their masses).

Monoclonal antibodies are large, complicated proteins that must then be separated and purified, first in centrifuges and after that with liquid chromatography.

Two - dimensional liquid chromatography - tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.

Liquid chromatography mass spectrometry (LCMS) was carried out using a Shimadzu LC - 20AD spectrometer at The Scripps Research Institute (La Jolla, CA, USA), and electrospray ionization (ESI) mass analysis with a Thermo Scientific LTQ Orbitrap - XL spectrometer at the Salk Institute.

The researchers combined protein purification techniques including fast protein liquid chromatography, with modern metagenomics, metaproteomic and associated bioinformatics analyses.

Brains that have been voxelated into ~ 700 cubes will be prepared using automated microscale sample processing and analyzed using high - throughput reversed - phase liquid chromatography coupled online with high - resolution mass spectrometry to obtain both the spatial localization and relative abundance of brain proteins.

These ultra-high-pressure liquid chromatography systems coupled with multiple switch valves and UV data acquisition makes it the system of choice for fully automated high - throughput approaches in the fields of metabolomics, proteomics, and food and environmental safety.

Liquid chromatography - mass spectrometry - based parallel metabolic profiling of human and mouse serum reveals putative biomarkers with the progression of nonalcoholic fatty liver diseases.

Samples were injected into a triple quadrupole, - MS / MS instrument (Thermo Scientific Finnigan TSQ Quantum UltraAM, San Jose, CA) after separation with an Ultra High Pressure Liquid Chromatography (Waters Acquity - UHPLC) system with a Binary Solvent Manager, Sample Manager and a Waters AccQTag Ultra column (part # 186003837, 2.1 x100 mm) at 55 °C.

Cells were then washed in medium, and metabolites were extracted with acetone and analyzed by reverse - phase high - performance liquid chromatography (HPLC) and by electrospray mass spectrometry as described previously (19).

As discussed in Part II of this review, urine hormone testing using both gas chromatography - mass spectrometry (GS - MS) or liquid chromatography - tandem mass spectrometry (LC - MS / MS) is well - established in the medical literature as a reliable method of assessing levels of active (free & conjugated) hormones and their metabolites, correlates well with patient symptoms, and reflects the beneficial impact (or lack of benefit and potentially oncogenic effect) of therapeutic interventions.43, 444 Radioimmunoassay (RIA) and enzyme - linked immunosorbent assay (ELISA, also known as enzyme immunoassay [EIA]-RRB- analysis may not be accurate.

Pooled fecal samples collected within the last week of each dietary period were prepared for analysis of neutral and acidic fecal sterols as described previously.20 Gas — liquid chromatography of neutral and acidic fecal sterols was performed on a gas chromatograph (model HP5890, Hewlett — Packard, Palo Alto, Calif.) equipped with an automatic sample injector.

Glycosylated hemoglobin was measured with ion - exchange high - performance liquid chromatography (Bio-Rad Laboratories, Hercules, Calif.).

For sample preparation, vitamin E was extracted from the serum, filtered, and analyzed with a liquid chromatography system equipped with fluorescence detector (Shimadzu, Columbia, MD, USA).

Highlights Maintained a GPA of 3.8 over six years of undergraduate and graduate study Completed upwards of 50 hours of lab work Familiar with several methods of sample analysis including liquid chromatography and gas chromatography Took field samples and maintained a sterile environment Friendly and respectful to teachers, supervisors and fellow students Punctual Skilled at note taking and independent research Organized Willing to learn, with an abiding interest in all aspects of the field of chemistry Experience Intern 8/1/2015 — 5/1/2016 Scientific Laboratories Incorporated — Syracuse, NY Took inventory of lab equipment Followed safety and security procedures accurately when handling hazardous material Worked closely with experienced chemists and earth scientists Cleaned and sanitized lab equipment Assisted with experiments Measured and mixed compounds for experienced chemists Established a mentor relationship with laboratory manager Assisted in the writing of technical reports

* Experience with sample preparation, titrations, micro-scale distillations, chemical synthesis and chemical analysis using instruments AA, ICP, UV - Vis, GC and GC - MS, and different liquid chromatography techniques.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign