Sentences with phrase «live cell microscopy»

The Z - project provides access to high / super-resolution and time - resolved live cell microscopy, and will provide training for students of the international research training group.

Dr. Taraska's lab studies the structural cell biology of exocytosis and endocytosis with advanced imaging methods including live cell microscopy, superresolution fluorescence, and electron microscopy.

In order to track the movements of biological particles in a cell, scientists at Heidelberg University and the German Cancer Research Center have developed a powerful analysis method for live cell microscopy images.

Not exact matches

The latest in live - cell microscopy — multiphoton imaging, light - sheet techniques, and technology borrowed from Raman spectroscopy — allow researchers to study living cells in more detail with less effort.

Eric Betzig, Stefan W. Hell and William E. Moerner share the 2014 chemistry Nobel for the development of super-resolved fluorescence microscopy, which has enabled the study of single molecules in ongoing chemical reactions in living cells.

In 2014 Stefan Hell, Eric Betzig, and William Moerner won for increasing the power of light microscopy and allowing scientists to see molecules in action within a living cell, although not at the level of atomic change.

Live cells are highly sensitive to their surroundings, so the new microscopy strategy — which replaces glass slides with blocks of collagen — could help reveal more natural behaviors.

Using cutting - edge 3D microscopy, researchers from the National Heart, Lung, and Blood Institute and Yale University examined the subcellular architecture of presynaptic terminals in retinal bipolar cells of live goldfish.

To better determine the role of specific chemoattractants in type III hypersensitivity, lead author Yoshishige Miyabe, MD, PhD, a research fellow in Luster's lab, used multiphoton intravital microscopy — an imaging technology pioneered for studies of immune cell movements in living animals by CIID investigator and co-author Thorsten Mempel, MD, PhD — to follow in real time the development of IC - induced arthritis in a mouse model of rheumatoid arthritis.

Scientists longing to sneak a peek at the molecular machinery of living cells came one step closer to that goal in March with the creation of lenses that break the limits of current light microscopy.

Researchers at Columbia University have reported a new approach to visualize glucose uptake activity in single living cells by light microscopy with minimum disturbance.

In this role, she works with many collaborators to facilitate implementing superresolution microscopy into their research programs as well as developing novel techniques for microbial live cell imaging.

«By combining in vivo multiphoton microscopy and in vivo electrophysiology, our lab is better able to visualize how cells move and change over time in the living brain and explain how changes in these glial cells alter the visually evoked neural network activity,» says Kozai.

learn from our speakers the benefits of imaging live cells using techniques such as high resolution microscopy, superresolution microscopy, and high - content analysis

Biologists commonly use fluorescence microscopy to study everything from embryo development to the intricate processes within living cells.

To find answers, Columbia researchers developed a new microscopy technique that allows for the direct tracking of fatty acids after they've been absorbed into living cells.

By making the switch, all molecules made from fatty acids can be observed inside living cells by an advanced imaging technique called stimulated Raman scattering (SRS) microscopy.

The authors of this study combined live cell imaging with electron microscopy to observe Trichoplax feeding behavior at scales ranging from the whole animal to subcellular.

Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy.

The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy.

The new methods dramatically improve on the spatial resolution provided by structured illumination microscopy, one of the best imaging methods for seeing inside living cells.

The structure of this complex, determined using cryo - electron microscopy, shows how it converts near - infrared light into an electrical charge in order to power cell metabolism, which enables this bacterium to live at the extreme red limit of photosynthesis on Earth.

By using video microscopy with fluorescent tagging of the two organelles, the scientists observed that the mitochondria and lysosomes formed stable contacts inside living human cells.

Current microscopy techniques can resolve details as small as 2 nanometres in prepared samples and 200 nanometres in living cells.

This was accomplished through the use of live - cell microscopy, microfluidic and imaging tools, and mathematical models.

An advancement in microscopy that provides an unprecedented understanding of the inner workings of live cells has won the 2014 - 2015 Newcomb Cleveland Prize of the American Association for the Advancement of Science (AAAS).

Powerful new microscopy techniques enable researchers to observe the whole process in living cells, with bright fluorescent tags highlighting the chromosomes and other cellular components.

The team has succeeded not only in deciphering what is happening in the cell interior but also, using a revolutionary live - cell microscopy technique, the scientists have observed for the first time individual receptors at work in intact cells.

Embryonic hemocytes lend themselves beautifully to live imaging studies since fluorescent probes can be expressed specifically in these cells using hemocyte specific promoters and their movements subsequently imaged within living embryos using confocal timelapse microscopy.

By developing a new fluorescence microscopy - based technique, the researchers were able to measure how long it takes proteins to move over distances ranging from 0.2 to 3 micrometres in living cells.

Research problems that are just out of reach today but that could be made accessible by advances in electron microscopy include studies of the little pores that form in our cells walls and which are centrally important in the regulation of all life processes as well as new nano - structured materials that are ultra-light yet strong, allowing reduced energy consumption in vehicles.

They then used super-resolution microscopy to prove that this computer - generated map matched up with the real - life chromosome organisation inside bacterial cells.

«I like it because you not only can control the viral dose infecting axons but also are able to monitor each step — from transport in axons to expression in the nucleus — by live - cell microscopy.»

In the first type, which was conducted at Rice, the team used confocal microscopy to film «giant unilamellar vesicles» (GUVs), synthetic membrane - enclosed structures that are about the same size as a living cell.

The Core's technical focus is on advanced microscopy, live cell imaging and molecular biology.

We will use high and super-resolution fluorescence microscopy like total internal reflection fluorescence microscopy (TIRFM) and fluorescence photo - activation localization microscopy (FPALM) to visualize and track the spatio - temporal dynamics of tethering and SNARE proteins in live and fixed cells with single molecule resolution.

«These fluorescence microscopy studies establish that the zinc spark occurs in human egg biology, and that can be observed outside of the cell,» said Professor Tom O'Halloran, a co-senior author and director of Northwestern University's Chemistry of Life Processes Institute, of a study that appeared in Scientific Reports.

Another Kavli researcher, Steven Siegelbaum, uses a technology called two - photon microscopy to image pre-synaptic terminals (the nerve cell tips that send charges from one cell to another) within slices of living brain tissue.

These questions will be addressed by combining unbiased «omics» - approaches (i.e. genomics, transcriptomics, and proteomics) and a targeted genetic analysis with both superresolution live - cell imaging and electron microscopy of nanotube - forming cells.

The researchers then used a dynamic force - scanning probe microscope for single - molecule force spectroscopy as well as antibody - recognition force microscopy (Ig - RFM) to map the locations of MtrC and OmcA on the surface of live Shewanella cells.

Additionally he is analysing protein - protein interactions in living cells using advanced microscopy.

The printed tissue constructs contain three types of living cells, which are labeled red, blue and green in the microscopy image (top) and schematic diagram (bottom).

Using single - molecule imaging, super-resolution microscopy and various biophysical and molecular approaches she will explore how gene expression in living cells works.»

Confocal microscopy of live cells.

A microscope and its parts, image formation, Köhler illumination, optical aberrations, types of lenses, phase contrast, interference contrast, polarization, fluorescence microscopy, laser confocal microscopy, two - photon confocal microscopy, superresolution microscopy, study of dynamic processes in living cells, immunofluorescence.

Förster resonance energy transfer microscopy and spectroscopy for localizing protein — protein interactions in living cells.

These include methods for deconvolution in fluorescence microscopy as applied to cellular morphogenesis, in addition to methods for live - cell binding measurements using fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single molecule tracking (SMT) as applied to transcription factor regulation.

Visualization of Rab5 activity in living cells by FRET microscopy and influence of plasma - membrane - targeted Rab5 on clathrin - dependent endocytosis.

Visualization of Rab5 activity in living cells using FRET microscopy.

Investigating protein - protein interactions in living cells using fluorescence lifetime imaging microscopy.