Not exact matches
... Researchers at Penn State University have found that three water soluble chemicals
in garlic reduced cholesterol production
in cultured rat
liver cells by 40 - 60 percent.
The researchers experimented with inducing oxidative stress
in a human
cell line
culture with and without VCOP (virgin coconut oil polyphenols) to observe how VCOP positively promoted catalase, a very important enzyme
in protecting the
cell from oxidative damage, and glutathione (GSH), a self - recycling antioxidant produced by the
liver.
In November 2010 Japanese researchers announced online in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
In November 2010 Japanese researchers announced online
in Analytical Chemistry that they had built a chip that simultaneously tests how liver, intestine and breast cancer cells respond to cancer drugs, and in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in Analytical Chemistry that they had built a chip that simultaneously tests how
liver, intestine and breast cancer
cells respond to cancer drugs, and
in February 2010 scientists publishing in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in February 2010 scientists publishing
in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe in cultured cell
in the Proceedings of the National Academy of Sciences USA developed a microscale replica of the human
liver that allowed them to observe the entire life cycle of hepatitis C, a virus that is difficult to observe
in cultured cell
in cultured cells.
The most promising chemical — sulforaphane, a naturally occurring compound found
in cruciferous vegetables — tamped down glucose production by
liver cells growing
in culture, and shifted
liver gene expression away from a diseased state
in diabetic rats.
The team pumped around 50 million rat
liver cells into each of five bare scaffolds, then incubated the organs
in culture for two weeks.
Cultures representing two other hereditary
liver diseases, alpha 1 — antitrypsin deficiency (a protein - folding disorder that leads to
cell death and
liver failure) and glycogen storage disease, type 1a (
in which a
liver enzyme deficiency impairs the body's glucose metabolism), also displayed disorder - specific abnormalities.
In a paper published in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated cell cultures to sustain hepatitis B virus in human liver cells, allowing them to study immune responses and drug treatment
In a paper published
in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated cell cultures to sustain hepatitis B virus in human liver cells, allowing them to study immune responses and drug treatment
in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated
cell cultures to sustain hepatitis B virus
in human liver cells, allowing them to study immune responses and drug treatment
in human
liver cells, allowing them to study immune responses and drug treatments.
They found that the infected
cultures responded to the drugs
in the same way that
liver cells inside the body are known to do.
For instance, when they added mosquito saliva containing sporozoites to
cultured mouse
liver cells, 10 % to 30 % of the
cells showed signs of punched membranes, the team reports
in the 5 January issue of Science.
As the researchers showed, the rate of vesicle formation, and hence the uptake of excess bile into
liver cells, can indeed be adjusted using drugs, at least
in the
cell culture setting.
«Our stem
cells also survive outside of mice,
in a
culture, so we can also manipulate them
in a laboratory,» said Abad, adding that: «The next step is studying if these new stem
cells are capable of efficiently generating different tissues such as that of the pancreas,
liver or kidney.»
In order to determine which type of
liver cells cause this accumulation, the researchers repeated the experiment using
cultured hepatocytes.
By adding a combination of four key factors, a skin
cell can be made into an iPSC, which can then be coaxed into forming
liver, lung and brain
cells in a
culture dish.
And when the researchers simulated starvation
in cultured mouse
liver cells, the
cells also ramped up SIRT1 production; when they added glucose,
cells ramped it down.
Other stem
cells in the body, such as
liver and stomach
cells, have been examined for their ability to
culture usable iPS
cells.
Human iPS
cell - derived hepatocytes differentiated with our robust differentiation protocol and
cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction
in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.
Notably, both
in hphep
cells and enhanced hiPS - HEP
cultures, only a subset of hepatocytes is strongly stained for glycogen storage — again
in agreement with the metabolic zonation observed
in the
liver lobe (Figure 3, Panel A).
Human iPS
cell - derived hepatocytes differentiated with our robust differentiation protocol and
cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction
in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.
Notably, both
in hphep
cells and the enhanced hiPS - HEP
cultures, only a subset of hepatocytes is strongly stained for glycogen storage (shown by Periodic acid - Schiff staining, below)-- again
in agreement with the metabolic zonation observed
in the
liver lobe.
Nakabayashi, Monk, and collaborators studied methylation patterns
in adult and umbilical blood and placenta
cells from healthy volunteers; brain tissue from a brain bank; and a
cultured liver cell line.
Of great interest, stem - like
cells have also been discovered
in some
cultured cancer
cell lines, including glioma (22, 23), ovarian (24, 25),
liver (26), and prostate (27 — 29) cancer
cells.
Although these data provide considerable evidence showing relevant functional characteristics of the
liver cells generated
in our
culture system, we acknowledge that these
cells were not terminally differentiated, as evidenced by their continued expression of AFP (Figure 1, B and C).
When he and others finally found the missing pieces, he learned that variations
in HCV genomes impeded the production of virus
in cultured liver cells.
While individual results may vary, both a
cell culture study and a clinical study
in dogs indicated Denamarin ®
liver health supplement can help protect and support the
liver.
ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (
liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (
liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (
liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and
liver disorders) Band band
cell — type of white blood
cell Baso basophil — type of white blood
cell Bile Acids digestive acids produced
in the
liver and stored
in the gall bladder (
liver function) Bili bilirubin (bile pigment responsible for jaundice from
liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and
liver function) Bx biopsy C & S aerobic / anaerobic bacterial
culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating
cells) Chol cholesterol (
liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood
cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood
cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (
liver disorders) Glob globulin (
liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood
cells Hb, Hgb hemoglobin — iron rich protein bound to red blood
cells that carries oxygen (anemia, red
cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed -
cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood
cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red
cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed -
cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood
cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood
cell — immature red blood
cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed -
cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet —
cells involved
in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood
cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood
cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (
liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood
cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration,
liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism,
liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood
cell count (infection, inflammation, bone marrow suppression)
In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung, liver, kidney, and spleen by cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence in situ hybridizatio
In cases of neonatal mortality, the diagnosis typically is made postmortem with virus isolation from fresh lung,
liver, kidney, and spleen by
cell culture techniques and subsequent identification by PCR and sequencing, transmission electron microscopy, immunofluorescence, or fluorescence
in situ hybridizatio
in situ hybridization.