Sentences with phrase «liver hepatocytes»

Of the many types of polarized epithelia, we have focused on liver hepatocytes, which exhibit a complex polygonal polarity, and simple columnar cells of the intestine.

Although the particles did penetrate endothelial cells in the liver, they did not enter liver hepatocytes.

Not exact matches

They transplanted the hepatocyte - like cells into mice; 14 days later, some of the corrected cells had integrated into the rodent liver and were able to produce human A1AT.

Gandhi's research team is the first to develop a mouse model with depleted levels of a protein called augmenter of liver regeneration (ALR), which is essential for the survival of liver cells called hepatocytes.

Further experimentation showed Lagasse that if he injected hepatocytes directly into the lymph nodes, the cells picked up signaling proteins (essentially SOS signals to grow) released into the bloodstream from the dying liver.

The scientists started with metabolically relevant cells — hepatocytes and adipocytes — representing major functions of the liver and adipose tissues, and thus important aspects of the body's energy processing and storing system.

Hepatocytes (liver cells) have the highest concentration of it - up to 8 % of the fresh weight in well fed state, or 100 - 120 g in an adult.

«We think the combination of making more hepatocytes and reducing collagen is the most promising approach to treating liver fibrosis,» says Willenbring.

Liver failure occurs when healthy cells called hepatocytes are damaged by alcohol and disease.

In the course of the inflammatory process, the liver cells (hepatocytes) die more frequently due to programmed cell death.

To validate their computer modeling predictions, researchers performed experiments in human cancer cell lines, mouse liver samples and primary human hepatocytes.

The researchers then tested the particles» ability to shut off the gene for a blood clotting protein called Factor VII, which is produced in the liver by cells called hepatocytes.

Anderson and Langer have previously developed nanoparticles, now in clinical development, that can deliver siRNA to liver cells called hepatocytes by coating the nucleic acids in fatty materials called lipidoids.

This colored scanning electron micrograph shows several specialized elements within a liver cell (hepatocyte).

The hepatocytes are first patterned onto surfaces dotted with tiny spots of collagen, and then surrounded by supportive tissue made up of stromal cells, which act as connective tissue and support the hepatocytes in carrying out their liver functions.

One uses primary hepatocytes obtained from livers donated for transplant; the second uses stem cells derived from human skin samples and guided into hepatocyte - like cells, Bhatia says.

«That's because the hepatocyte — the main cell in the liver — is unstable,» she says.

This involved culturing two liver cells (hepatocytes) that can act as a functional organ unit on artificial membranes fabricated with microwell patterns.

«Investigators have been developing in - vitro liver models for 40 years, but all of those systems ignore the distinct patterns of metabolically active hepatocytes that exist within the liver sinusoid» says Martin Yarmush, MD, PhD, director of the MGH - CEM and the paper's senior author.

In order to determine which type of liver cells cause this accumulation, the researchers repeated the experiment using cultured hepatocytes.

Without CPEB4, the endoplasmic reticulum is unable to activate the stress response, thus causing hepatocytes to accumulate the lipids produced by the fatty liver.

«Liver tissue model accurately replicates hepatocyte metabolism, response to toxins: Microfluidic device may help predict toxic effects of new drugs, study liver disease.&rLiver tissue model accurately replicates hepatocyte metabolism, response to toxins: Microfluidic device may help predict toxic effects of new drugs, study liver disease.&rliver disease.»

In order to develop a system that more closely replicates the metabolic differences among hepatocytes, the research team developed a microfluidic device that distributes hormones or other chemical agents across a 20 - to 40 - cell - wide sample of hepatocytes in such a way that the effects on the liver cells vary from one side to the other.

In this schematic of the new «liver on a chip» device, adding blood - sugar - raising glucagon to inlet 1 and blood - sugar - lowering insulin to inlet 2 distributes the hormones across the field of hepatocytes (purple box) along opposing gradients, altering the cells» metabolism from glucose release on the left side to glucose uptake on the right.

When blood passes through the liver, it travels from arteries to veins through channels called sinusoids, lined with the liver cells called hepatocytes.

«As mature hepatocytes die off, these clones replace the liver mass.

Non-alcoholic fatty liver is characterised by the accumulation of fat deposits in hepatocytes.

The research also detected the proliferation of binucleate hepatocytes a sign that the liver is attempting to rectify damage caused by a low protein diet.

The liver's cells, called hepatocytes, work to filter and remove toxins from the blood.

When Lin engineered the telomerase - expressing hepatocytes to die in response to a chemical signal and gave the mice with a liver - damaging chemical, he found that those animals in which the telomerase cells had been killed exhibited much more severe liver scarring than those in which the cells were functional.

«We have shown that an endogenous peptide, catestatin, can directly suppress glucose production from hepatocytes and can indirectly suppress lipid accumulation in liver as well as macrophage - mediated inflammation in obese mice,» said Sushil K. Mahata, PhD, professor of medicine at UC San Diego School of Medicine.

However, Takebe's liver bud has the advantage of being grown from iPS cells, rather than, for example, the primary human hepatocytes used in Bhatia's graft, which could make it useful in modelling rare diseases or examining the specific genetic backgrounds of the iPS cell donors.

Research Interests: alcoholic liver disease; macrophages; C1q; apoptosis; fibrosis; liver disease; alcohol; Heme - oxygenase - 1; inflammation; necroptosis; high - fat diet; liver injury; fatty liver disease; complement; hepatocytes; alcoholic hepatitis; hepatic stellate cell; adiponectin; MIF; carbon tetrachloride

In our previous study we found that a high fat diet containing comparable amounts of soybean oil to what Americans are currently consuming caused mice to become obese, diabetic and insulin resistant and to have large lipid droplets and hepatocyte ballooning in their livers.

Their livers also had less fat than either of the two soybean oil diets and showed no signs of hepatocyte ballooning.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Rat Hepatocyte Culture Model of Macrosteatosis: Effect of Macrosteatosis Induction and Reversal on Viability and Liver - specific Function.

Notably, both in hphep cells and enhanced hiPS - HEP cultures, only a subset of hepatocytes is strongly stained for glycogen storage — again in agreement with the metabolic zonation observed in the liver lobe (Figure 3, Panel A).

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Notably, both in hphep cells and the enhanced hiPS - HEP cultures, only a subset of hepatocytes is strongly stained for glycogen storage (shown by Periodic acid - Schiff staining, below)-- again in agreement with the metabolic zonation observed in the liver lobe.

Another liver - specific protein, Asialoglycoprotein receptor 1 (ASGPR1), is a surface marker for mature hepatocytes [5, 6].

Another characteristic used to evaluate hepatocyte models is the presence of functional alpha -1-antitrypsin (α1AT), a protease inhibitor produced in the liver.

Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor required for liver development and the control of expression of liver - specific genes, and it is associated with several critical metabolic pathways [3].

We combine experimental and computational approaches to investigate the molecular mechanisms underlying hepatocyte polarity as well as the 3D organization of the bile canaliculi and sinusoidal networks in the mouse and human liver tissue.

Only about 6 % of hepatocytes, or liver cells, were edited by CRISPR (Nat.

For example, administration of hepatocytes toward treatment of liver diseases is being tested.

The Liver - Chip showed improved viability and hepatic function compared to conventional hepatocyte culture systems over long - term culture.

To highlight the potentially important role of 3D culture conditions, the authors compared 2D monolayers of hepatocyte progenitors, fetal liver stromal cells, or adult bone marrow - derived stromal progenitor cells to their 3D counterparts cultured on liver extracellular matrix (3D - ECM) constructs.

They developed a simple system that allows them to transplant human hepatocytes into immunodeficient mice, which can now be used to test how drugs affect the liver.

The usual approach is to grow human cells in a dish, to infect and try to treat them there, but this is not possible with liver cells or hepatocytes.